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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | Latino oral health: a re- search agenda toward eliminating oral health disparities 显示文摘 | Ramos-Gomez F Cruz GD Watson MR | 2005 | J Am Dent Assoe2005，136，12： | 1 |
| 3 | Meningeal carcinomatosis in breast cancer:prognostic factors and outcome显示文摘 | de Azevedo CR Cruz MR Chinen LT | | 0，，02： | 1 |
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| 7 | Synthesis of alpha 1- antichymotrypsin and alpha l-antitrypsin by human trophoblast 显示文摘 | Bergman D Kadner SS Cruz MR | 1993 | Pediatr Res1993，34，3： | 1 |
| 8 | Combination of afatinib with cetuximab in patients with EGFR-mutant non-small-cell lung cancer resistant to EGFR inhibitors 显示文摘 | Ribeiro Gomes J Cruz MR | 2015 | Onco Targets Ther2015，8，： | 1 |
| 9 | Treatment of primary cardiac malignancies with orthotopic heart transplantation 显示文摘 | Rodriguez Cruz Cintron MR Forbes TJ | 2000 | Bol Asoc Med P R2000，92，： | 1 |
| 10 | Combination of afatinib with cetuximab in patients with EGFR-mutarlt non-small-cell lung cancer resistant to EGFR inhibitors 显示文摘 | Ribeiro Gomes J Cruz MR | 2015 | Onco Targets Ther2015，8，： | 1 |
| 11 | PubChem promiscuity: a web resource for gathering compound promiscuity data from PubChem 显示文摘 | Canny SA Cruz Southern MR | 2012 | Bioinformatics2012，28，： | 1 |
| 12 | PubChem prom- iscuity: a web resource for gathering compound promiscuity data from PubChem 显示文摘 | Canny SA Cruz Y Southern MR | 2012 | Bioinformatics2012，28，1： | 1 |
| 13 | Presentation form and evolution of double chambered right ventricle显示文摘 | Yabur-Espitia MR Patifio-Bahena E Cruz Prieto B et al | 2013 | Arch Cardiol Mex2013，83，1： | 1 |
| 14 | Longitudinal changes in uterine, umbilical and fetal cerebral Doppler indices in late- onset small- for- gestational age fetuses 显示文摘 | Oros D Figueras F Cruz MR | 2011 | Uhrasound Obstet Gynecol2011，37，2： | 1 |
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