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| 1 | Immunogenicity analysis following human immunodeficiency virus recombinant DNA and recombinant vaccinia virus Tian Tan prime-boost immunization显示文摘This study assessed and compared the immunogenicity of various immunization strategies in mice using combinations of recombinant DNA(pCCMp24) and recombinant attenuated vaccinia virus Tian Tan(rddVTT-CCMp24).Intramuscular immunization was performed on days 0(prime) and 21(boost).The immunogenicity of the vaccine schedules was determined by measuring human immunodeficiency virus(HIV)-specific binding antibody levels and cytokine(interleukin-2 and interleukin-4) concentrations in peripheral blood,analyzing lymphocyte proliferation capacity against HIV epitopes and CD4 + /CD8 + cell ratio,and monitoring interferon-gamma levels at different times post-immunization.The results showed that pCCMp24,rddVTT-CCMp24 and their prime-boost immunization induced humoral and cellular immune responses.The pCCMp24/rddVTT-CCMp24 immunization strategy increased CD8 + T cells and induced more IFN-γ-secreting cells compared with single-shot rDNA.The prime-boost immunization strategy also induced the generation of cellular immunological memory to HIV epitope peptides.These results demonstrated that prime-boost immunization with rDNA and rddVTT-CCMp24 had a tendency to induce greater cellular immune response than single-shot vaccinations,especially IFN-γ response,providing a basis for further studies. | LIU CunXia DU ShouWen LI Chang WANG YuHang WANG MaoPeng LI Yi YIN RongLan LI Xiao REN DaYong QIN YanQing REN JingQiang JIN NingYi | 2013 | Science China(Life Sciences)2013，56，6： | 7 |
| 2 | Efficacy of seasonal pandemic influenza hemagglutinin DNA vaccines delivered by electroporation against aseasonal H1N1 virus challenge in mice显示文摘Prophylactic DNA vaccines against the influenza virus are promising alternatives to conventional vaccines.In this study,we generated two candidate gene-based influenza vaccines encoding either the seasonal or pandemic hemagglutinin antigen(HA) from the strains A/New Caledonia/20/99(H1N1)(pV1A5) and A/California/04/2009(H1N1)(pVEH1) ,respectively.After verifying antigen expression,the immunogenicity of the vaccines delivered intramuscularly with electroporation was tested in a mouse model.Sera of immunized animals were tested in hemagglutination inhibition assays and by ELISA for the presence of HA-specific antibodies.HA-specific T-cells were also measured in IFN-γELISpot assays.The protective efficacy of the candidate influenza vaccines was evaluated by measuring mortality rates and body weight after a challenge with 100 LD50 of mouse-adapted A/New Caledonia/20/99(H1N1) .Mice immunized with either one of the two vaccines showed significantly higher T cell and humoral immune responses(P<0.05) than the pVAX1 control group.Additionally,the pV1A5 vaccine effectively protected the mice against a lethal homologous mouse-adapted virus challenge with a survival rate of 100%compared with a 40%survival rate in the pVEH1 vaccinated group(P<0.05) .Our study indicates that the seasonal influenza DNA vaccine completely protects against the homologous A/New Caledonia/20/99 virus(H1N1) ,while the pandemic influenza DNA vaccine only partially protects against this virus. | TAN Lei LU HuiJun ZHANG Dan WANG KaiYan TIAN MingYao LIU CunXia LIU YanYu HU Bo JIN NingYi | 2011 | Science China(Life Sciences)2011，54，4： | 2 |
| 3 | Improved electrical properties of Ge p-MOSFET with HfO2 gate dielectric by using TaOxNy interlayer显示文摘 | Xu Jingping Zhang Xuefeng Li Cunxia | 2008 | IEEE Electron Device Lett2008，29，10： | 1 |
| 4 | Improved electrical properties of Ge metal-oxide-semiconductor capacitor with HfTa-based gate dielectric by using TaOxNy interlayer显示文摘 | Zhang Xuefeng Xu Jingping Li Cunxia | 2008 | Appl Phys Lett2008，92，26： | 1 |
| 5 | Improving oxidation resistance and thermal insulation of thermal barrier coatings by intense pulsed electron beam irradiation显示文摘 | Xianxiu Mei Xiaofei Liu Cunxia Wang Younian Wang Chuang Dong | 2012 | Applied Surface Science2012，，： | 1 |
| 6 | Candidate Genes Polymorphism andlts Association to Prolificacy in Chinese Goats显示文摘 | Xiaoke Ma Xiaoyong Liu Cunxia Zhang | 2010 | Agricultural Science2010，2，1： | 1 |
| 7 | Fabrication of three-di- mensional microfluidic channels in glass by femtosecond pulses 显示文摘 | Li Cunxia Shi Xu Si Jinhai | 2009 | Optics Communications2009，，282： | 1 |
| 8 | Improved electrical properties of Ge p-MOSFET with HfO2 gate dielectric by using TaOxNy interlayer显示文摘 | Xu Jingping Zhang Xuefeng Li Cunxia | 2008 | IEEE Electron Device Lett2008，29，10： | 1 |
| 9 | Xiaoke Decoction in treatment of type Ⅱ diabetes:A Meta-analysis显示文摘Objective:To systematically evaluate the clinical effect of Xiaoke Decoction in the treatment of type 2 diabetes.Methods:Chinese databases such as CNKI,Wanfang,Weipu Chinese Biomedical Journal Database,and Chinese Medical Biological Literature Database,Pub Med,Cochrane Library,Embase,and Web of Science were searched for English language literature from their inception until November 2019.A Meta-analysis was performed using Rev Man 5.3 and Stata 12.0.Results:Thirty-eight studies were included in this study,with a total of 3757 patients.It was found that adding Xiaoke Decoction could improve total efficiency.The Xiaoke Decoction groups surpassed the western medicine groups regarding improvement in total efficiency(OR = 3.49;95% CI:2.78–4.39,P < 0.00001).Adding Xiaoke Decoction could lower the fasting plasma glucose(FPG) level.The Xiaoke Decoction groups surpassed the western medicine groups regarding reduction in FPG levels(MD =-1.14;95% CI:-1.36 to 0.92,P < 0.00001).Adding Xiaoke Decoction could lower the 2 h postprandial blood glucose(2 h PG) level.The Xiaoke Decoction groups surpassed the western medicine groups regarding reduction in 2 h PG(MD =-1.40;95% CI:-1.61 to 1.19,P < 0.00001).Adding Xiaoke Decoction could lower glycated hemoglobin(Hb A1 c).The Xiaoke Decoction groups surpassed the western medicine groups regarding reduction in Hb A1 c(MD =-0.77;95% CI:-0.95 to 0.58,P < 0.00001).It was found that adding Xiaoke Decoction could lower the traditional Chinese medicine(TCM) syndrome score.The TCM syndrome scores among patients in the Xiaoke Decoction group were lower than those among patients in the control group after treatment(MD =-4.90;95% CI:-7.22 to 2.57,P < 0.0001).At the same time,we conducted a subgroup and sensitivity analysis of age and intervention duration on the heterogeneity of total efficiency,FPG,2 h PG,Hb Alc,and TCM syndrome score outcome indicators.For detecting publication bias,an egger test was conducted.Conclusion:Compared with western medicine alone,Xiaoke Decoction has more advantages for the treatment of type 2 diabetes with respect to total efficiency,FPG,2 h PG,Hb Alc,and TCM syndrome score. | Baochang Zhou Guoqing Zhang Wenfang Guo Cunxia Ren Minhui Li | 2022 | Chinese Herbal Medicines2022，14，1： | 0 |
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