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| 1 | Isolation and characterization of class Ⅰ MHC genes in the giant panda(Ailuropoda melanoleuca)显示文摘Artificial breeding is an important project to protect,recover and reintroduce endangered species.Knowledge of the population's genetic diversity at functional loci is important for the establishment of effective captive breeding programs.The major histocompatibility complex(MHC) genes are ideal candidate genetic markers to inform planned breeding,due to their high levels of polymorphism and importance in the main immune coding region of the vertebrate genome.In this study,we constructed BAC-based contigs and isolated six functional MHC class Ⅰ genes from the giant panda(Ailuropoda melanoleuca),which we designated Aime-C,Aime-F,Aime-I,Aime-K,Aime-L and Aime-1906.Analyses of the tissue expression patterns and full-length cDNA sequences of these class I genes revealed that Aime-C,-F,-I and-L could be considered classical class Ⅰ loci,due to their extensive expression patterns and normal exonic structures.In contrast,Aime-K and-1906 appeared to be nonclassical genes based on their tissue-specific expression patterns and the presence of an abnormal exon 7 in both genes.We established techniques for genotyping exons 2 and 3 of the classical loci using locus-specific single strand conformation polymorphism(SSCP) and sequence analysis.In the Chengdu captive population,we identified one monomorphic locus(Aime-F) and three polymorphic loci with different numbers of alleles(4/4/4 exon 2 alleles at Aime-C/I/L and 6/5/5 exon 3 alleles at Aime-C/I/L).The distributions of the Aime-C,-I and-L alleles among members of different families were in good agreement with the known pedigree relationships,suggesting that the genotyping results are reliable.Therefore,the MHC-I genotyping techniques established in this study may provide a powerful tool for the future design of scientific breeding or release/reintroduction programs. | ZHU Ying SUN DanDan GE YunFa YU Bin CHEN YiYan WAN QiuHong | 2013 | Chinese Science Bulletin2013，58，17： | 5 |
| 2 | Neurological complications and infection mechanism of SARS-CoV-2显示文摘Currently,SARS-CoV-2 has caused a global pandemic and threatened many lives.Although SARS-CoV-2 mainly causes respiratory diseases,growing data indicate that SARS-CoV-2 can also invade the central nervous system(CNS)and peripheral nervous system(PNS)causing multiple neurological diseases,such as encephalitis,encephalopathy,Guillain-Barrésyndrome,meningitis,and skeletal muscular symptoms.Despite the increasing incidences of clinical neurological complications of SARS-CoV-2,the precise neuroinvasion mechanisms of SARS-CoV-2 have not been fully established.In this review,we primarily describe the clinical neurological complications associated with SARS-CoV-2 and discuss the potential mechanisms through which SARS-CoV-2 invades the brain based on the current evidence.Finally,we summarize the experimental models were used to study SARS-CoV-2 neuroinvasion.These data form the basis for studies on the significance of SARS-CoV-2 infection in the brain. | Dandan Wan Tingfu Du Weiqi Hong Li Chen Haiying Que Shuaiyao Lu Xiaozhong Peng | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 4 |
| 3 | Nonlinear oscillation of pathological vocal folds during vocalization显示文摘The extended two-mass model is adopted to analyze the nonlinear oscillation of pathological vocal folds during vocalization. Redundant tissue or area in laryngeal patients is modeled as a massless rigid connected to the upper mass of the vocal folds, and a parameter Q is introduced to represent the change of glottal configurations and tension imbalance between the left and right sides of vocal folds. Numerical simulations demonstrate that the pathological vocal-fold decreases the threshold of Q to generate nonlinear vocal oscillation, indicating the improvement of the sensitivity of vocal folds to asymmetries and enhancing the coupling between two sides. Furthermore, the pathological vocal-fold can lower the fundamental frequency and eliminate high-order harmonics, For example, the fundamental frequency decreases from 119.94 Hz to 84.95 Hz when Q=0.58 and the sub-glottal pressure 1450 Pa. However, there are no prominent effects on the amplitudes of sub-harmonic and low-order harmonics. | WAN Ni PENG DanDan SUN Min ZHANG Dong | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，7： | 1 |
| 4 | Estrogen and pure antiestrogen fulvestrant (ICI 182 780) augment cell–matrigel adhesion of MCF-7 breast cancer cells through a novel G protein coupled estrogen receptor (GPR30)-to-calpain signaling axis显示文摘 | Yan Chen Zheng Li Yan He Dandan Shang Jigang Pan Hongmei Wang Huamei Chen Zhuxia Zhu Lei Wan Xudong Wang | 2014 | Toxicology and Applied Pharmacology2014，，： | 1 |
| 5 | Design, Syntheses and Biological Activities of Novel Sulfonylureas Containing an Oxime Ether Moiety显示文摘 | WEI Wei CHENG Dandan CHEN Wei LIU Jingbo WAN Yingying LI Yuxin LI Yonghong YU Shujing LI Zhengming | 2016 | Chemical Research in Chinese Universities2016，32，2： | 1 |
| 6 | Contamination trends of trace metals and coupling with algal productivity in sediment cores in Pearl River Delta, South China显示文摘 | Dandan Duan Yong Ran Hefa Cheng Jing’an Chen Guojiang Wan | 2013 | Chemosphere2013，，： | 1 |
| 7 | Tripterin liposome relieves severe acute respiratory syndrome as a potent COVID-19 treatment显示文摘For coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),15–30%of patients are likely to develop COVID-19-related acute respiratory distress syndrome(ARDS).There are still few effective and well-understood therapies available.Novel variants and short-lasting immunity are posing challenges to vaccine efficacy,so finding antiviral and antiinflammatory treatments remains crucial.Here,tripterin(TP),a traditional Chinese medicine,was encapsulated into liposome(TP lipo)to investigate its antiviral and antiinflammatory effects in severe COVID-19.By using two severe COVID-19 models in human ACE2-transgenic(hACE2)mice,an analysis of TP lipo’s effects on pulmonary immune responses was conducted.Pulmonary pathological alterations and viral burden were reduced by TP lipo treatment.TP lipo inhibits SARS-CoV-2 replication and hyperinflammation in infected cells and mice,two crucial events in severe COVID-19 pathophysiology,it is a promising drug candidate to treat SARS-CoV-2-induced ARDS. | Haiying Que Weiqi Hong Tianxia Lan Hao Zeng Li Chen Dandan Wan Zhenfei Bi Wenyan Ren Min Luo Jingyun Yang Cai He Ailing Zhong Xiawei Wei | 2023 | Signal Transduction and Targeted Therapy2023，8，1： | 1 |
| 8 | HIF1α epigenetically repressed macrophages via CRISPR/Cas9-EZH2 system for enhanced cancer immunotherapy显示文摘Immune suppressive microenvironment in tumor emerges as the main obstacle for cancer immunotherapy.In this study,we identified that HIF1α was activated in the tumor associated macrophages and acted as an important factor for the immune suppressive microenvironment.Epigenetically silencing of Hif1αvia histone H3 methylation in the promoter region was achieved by CRISPR/dCas9-EZH2 system,in which histone H3 methylase EZH2 was recruited to the promoter region specifically.The Hif1αsilenced macrophage,namely HERM(Hif1αEpigenetically Repressed Macrophage)manifested as inheritable tumor suppressing phenotype.In the subcutaneous B16-F10 melanoma syngeneic model,intratumoral injection of HERMs reprogrammed the immune suppressive microenvironment to the active one,reducing tumor burden and prolonging overall survival.Additionally,HERMs therapy remarkably inhibited tumor angiogenesis.Together,our study has not only identified a promising cellular and molecular target for reverting immune suppressive microenvironment,but also provided a potent strategy for reprogramming tumor microenvironment via epigenetically reprogrammed macrophages. | Yan Dong Siyan Zhang Xiaotong Gao Dandan Yin Tingting Wang Zhelong Li Zhuo Wan Mengying Wei Ying Luo Guodong Yang Li Liu | 2021 | Bioactive Materials2021，6，9： | 1 |
| 9 | Clinical correlations with disease-associated auto-antibodies in a Chinese cohort with systemic sclerosis显示文摘To the Editor:Systemic sclerosis(SSc)is an autoimmune disease characterized by,progressive skin and visceral fibrosis,microvasculopathy,and autoimmunity.Circulating auto-antibodies(AAbs)are detectable in 90%to 95%of patients with SSc. | Sen Yang Minrui Liang Chen Chen Wenjing Ye Xiaoxia Zhu Yu Xue Ning Kong Yiyun Yu Dandan Xuan Shucong Zheng Xue Yang Zaihua Zhu Tianyi Zhao Weiguo Wan Hejan Zou | 2022 | Chinese Medical Journal2022，，15： | 0 |
| 10 | Solid Material Formula of Small Fragrant Chicken Soup Stock and Improvement of Boiling Process显示文摘[Objectives]To study the solid material formula and boiling process of Small Fragrant Chicken Soup Stock.[Methods]Small fragrant chicken and edible fungi were used as the main raw materials to prepare instant chicken and Morchella esculenta soup stock,and the influencing factors such as solid material ratio,ratio of liquid to material,time and temperature were studied,and the optimal formula and process of the soup stock were screened out through sensory and instrument evaluation experiments.[Results]The optimal formula and process of Small Fragrant Chicken Soup Stock were as follows:small fragrant chicken 40%,M.esculenta 6%,Lyophyllum decastes 3%and Lentinus edodes 1.5%;the ratio of liquid to material was 2.5:1,the boiling time was 2 h,and the boiling temperature was 120℃.Under these conditions,the sensory score of Small Fragrant Chicken Soup Stock was the best.[Conclusions]This study provided a theoretical basis for the industrial production of edible mushroom prepared dishes. | Chaohang CHEN Dandan LU Maozhao WU Haiyan WANG Jie XIE Guanghui YANG Shoumei WAN Lingling XIAO Shengxian PAN Jinyu ZHAO | 2023 | Asian Agricultural Research2023，15，10： | 0 |
| 11 | China Biodiversity Observation Network for better global sustainability developments显示文摘Biodiversity is undergoing unprecedented changes characterized by long-term,complex,and lagging consequences[1].Understanding the dynamic changes of biodiversity is essential for the conservation of biodiversity[2].Biodiversity monitoring is periodic measurements of biodiversity within a given area,such as data collection regarding the structure and function of ecosystems,the composition and distribution of species,and environmental factors.Based on the collected data,analyses could be performed to determine the trends of biodiversity change and potential drivers and assist in the evaluation of conservation effectiveness and decision-making[2,3]. | Jianfeng Yi Fangzhou Ma Jiaqi Li Wei Liu Yun Cao Yaqiong Wan Chenbin Wang Mengmeng Chen Dandan Yu Haigen Xu | 2022 | Eco-Environment & Health2022，1，4： | 0 |
| 12 | Alkali Tolerance of Concrete Internal Curing Agent Based on Sodium Carboxymethyl Starch显示文摘Internal curing agents (ICA) based on super absorbent polymer have poor alkali tolerance and reduce the early strength of concrete.An alkali tolerate internal curing agent (CAA-ICA) was designed and prepared by using sodium carboxymethyl starch (CMS) with high hydrophilicity,acrylic acid (AA) containing anionic carboxylic group and acrylamide (AM) containing non-ionic amide group as the main raw materials.The results show that the ratio of CAA-ICA alkali absorption solution is higher than that existing ICA,which solves the low water absorption ratio of the ICA in alkali environment.The water absorption ratio of CAA-ICA in saturated Ca(OH)_(2) solution is 95.8 g·g^(-1),and the alkali tolerance coefficient is 3.4.The application of CAA-ICA in cement-based materials can increase the internal relative humidity and miniaturize the pore structure.The compressive strength of mortar increases up to 12.95%at 28 d,which provids a solution to overcome the reduction of the early strength. | 陈梅花 刘荣进 CHEN Ping JING Daiyan WAN Dandan FU Siyuan | 2024 | Journal of Wuhan University of Technology(Materials Science)2024，39，1： | 0 |
| 13 | PRKG1 mutation identified by whole-exome sequencing:a potential genetic etiology for He-Zhao deficiency显示文摘Objective:He-Zhao deficiency was originally described as a severe type of nonsyndromic hypodontia,and the causative gene locus was mapped to chromosome 10q11.2.The aim of this study was to identify potential genetic mutations that could cause He-Zhao deficiency.Methods:Patients with He-Zhao deficiency and their unaffected relatives of the large pedigree were investigated.The whole-exome sequencing using next-generation sequencing was employed to identify genetic variants.The data generated from the whole-exome sequencing using the Illumina Novaseq 6000 system were further analyzed by Burrows-Wheeler Aligner software,Sequence Alignment/Map tools and ANNOVAR tool.In vitro luciferase assay was used to investigate the effect of the detected mutation on gene expression.R environment was used to conduct t-tests.The study protocol was approved by the Research Ethics Committee of Bio-X Institutes,Shanghai Jiao Tong University(M2011004).Results:The exomes of five patients with He-Zhao deficiency and two of their unaffected relatives identified a mutation in PRKG1αas the molecular etiology of the disease.The variant c.-144 C>A of PRKG1 isoform 1 cosegregated with permanent tooth agenesis in 93 family members who were older than 12,at which time the primary teeth should have been replaced with permanent teeth.Functional studies suggested that the mutant allele promotes gene transcription by increasing its promoter activity.Conclusion:c.-144 C>A variant of PRKG1αinvolving odontoclast-associated root resorption is responsible for He-Zhao deficiency,unlike other forms of hypodontia,which typically involve odontoblast dysfunction. | Xiaowen Hu Dandan Wang Xuhan Yang Zhongchen Song Zuolin Wang Juan Zhang Chunling Wan Lin He | 2022 | Journal of Bio-X Research2022，5，3： | 0 |
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