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59篇 您的检索式:作者名="Darinka"
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1Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays.Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson 2015World Journal of Gastroenterology2015,21,11:2
2Fast and easy muhiresidue method employing acetonitrile extraction/partitioning and ' dispersine - phase extraction' for the determi- nation of pesticide residues in product 显示文摘Michelangelo Anastassiaoles Steven J Lehotay Darinka Stajnbaher AOAC0,86,2:1
3An overview of metals recovery from thermal power plant solid wastes显示文摘Amr S. Meawad Darinka Y. Bojinova Yoncho G. Pelovski 2010Waste Management2010,,12:1
4Multiresidue method for determination of 90 pesticides in freshfruits and vegetables using solid-phase extraction and gas chromatography-mass spectrometry显示文摘Darinka stajnbaher Lucija Zupancic Kralj 2003Journal of Chromatography A2003,1015,:1
5Multivariate data analysis in classification of vegetable oils characterized by the content of fatty acids 显示文摘Darinka Brodnjak-Voncina Zdenka Cencic Kodba Marjana Novic 2005Chemometrics and Intelligent Laboratory Systems2005,75,3:1
6Fast and easy multiresidue method employing acetonitrile extraction/ partitioning and 'dispersive solid-phase extraction' for the determination of pesticide residues in produce显示文摘Michelangelo A Steven J L Darinka S 2003J AOAC Int2003,86,2:1
7Multiresidue method for determination of 90 pesticides in fresh fruits and vege- tables using solid-phase extraction and gas chromatog- raphy-mass spectrometry显示文摘DARINKA S LUCIJA Z 2003J Chromatogr A2003,1015,1:1
8Fast and easy muhiresidue method employing aeetonitrile extraction/ partitioning and 'dispersive solid-phase extraction' for the determinationof pesticide residues in produce 显示文摘Michelangelo A Steven J L Darinka S 2003JAOAC Int2003,86,2:1
9Fast and Easy Multiresidue Method Employing Acetonitrile Extraction /Partitioning and 'Dispersive Solid-phase Extraction' for the Determination of Pesticide Residues in Product 显示文摘Michelangelo Anastassiades Stcven J Lehotay Darinka Stajnbaher 2003J AOAC lnt2003,86,2:1
10Fast and easy multiresidue method employing acetonitrile extracion/partitioning and 'dispersive solid-phase extracion' for the determination of pesticide residues in produce显示文摘Michelangelo Anastassiades Steven J lehotay Darinka Stajnbaher 2003Journal of Aoac International2003,86,2:1
11Fast and easy muhiresidue method employing acetonitrile extraction/partitioning and dispersive solid - phase extraction for the determination of pesticide residues in product 显示文摘Michelangelo Anastassiades Steven J Lehotay Darinka Stajnbaher 2003J AOAC Int2003,86,:1
12Effect of phosphorus fertilization on Zn and Cd contents in soil and corn plants显示文摘Darinka Bogdanovic Momcilo Ubavic Maja Cuvardic 1999Nutrient Cycling in Agroecosystems1999,,1:1
13Residual dyebath purification using a system of constructed wetland 显示文摘Alenka Ojstrsek Fakin Darinka Danijel Vrhovsek 2007Dyes Pigments2007,74,3:1
14Fast and easy multiresidue method employing acetonitfile extraction/partitioning and 'dispersive solid-phase extraction'for the determination of pesticide residues in produce显示文摘MICHELANGELO A STEVEN J L DARINKA S 2003AOAC International2003,86,2:1
15Effect of phosphorus fertilization on Zn and Cd contents in soil and corn plants显示文摘Darinka Bogdanovic Momcilo Ubavic Maja Cuvardic 1999Nutrient Cycling in Agroecosystems1999,,1:1
16Residual Dyebath Purification Using a System of Constructed Wetland 显示文摘Alenka Ojstrsek Darinka Fakin Danijel Vrhovsek 2007Dyes and Pigments2007,,74:1
17Colour of flax fibres in regard to different pretreatment and dyeing processes 显示文摘Darinka Fakin Alenka Ojstrsek 2008Coloration Technology2008,,124:1
18Influence of the stiffness of three-dimensionalalginate/collagen-I interpenetrating networks on fibroblast biology显示文摘Cristiana B da C Darinka D K Li W W A 2014Biomaterials2014,35,32:1
19Multiresidue method for determination of 90 pesticides in fresh fruits and vegetables using solid-phase extraction and gas chromatography-mass spectrometry显示文摘Darinka ?tajnbaher Lucija Zupan?i?-Kralj 2003Journal of Chromatography A2003,,1:1
20An overview of metals recovery from thermal power plant solid wastes 显示文摘Meawad Amr S Bojinova Darinka Y Pelovski Yoncho G 2010Waste Management2010,30,12:1
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