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| 1 | Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone显示文摘Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody(mAb) probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid(LTA). Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PET imaging in an in vivo mouse model of prosthetic joint infection(PJI). In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography(PET) with [^(18)F]FDG and [^(18)F]Na F as well as X-ray computed tomography(CT), before being evaluated with the zirconium-89-labeled antibody specific for LTA([^(89)Zr]SAC55).The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater(P < 0.05) uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^(89)Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI. | Julie E.Pickett John M.Thompson Agnieszka Sadowska Christine Tkaczyk Bret R.Sellman Andrea Minola Davide Corti Antonio Lanzavecchia Lloyd S.Miller Daniel LJ Thorek | 2018 | Bone Research2018，6，2： | 3 |
| 2 | A human lymphocyte homging, receptor, the homes antigen, is related to cartilage proteoglycancore and link proteins显示文摘 | Goldstein LA David FH Picker LJ | 2010 | Cell2010，56，6： | 1 |
| 3 | Susceptibility of ovine lens crystallins to proteolytic cleavage during formation of hereditary cataract显示文摘 | Robertson LJ David LL Riviere MA | 2008 | Invest Ophthalmol Vis Sci2008，49，3： | 1 |
| 4 | 3D conformal radiotherapy for gastric cancer: Results of a comparative planning study显示文摘 | Trevor L David W Daryl LJ | 2005 | Radiot Oncol2005，74，3： | 1 |
| 5 | The glycemic index:methodology and clinical implication显示文摘 | Wolever TMS David JA Alexandra LJ | 1991 | Am J Clin Nutr1991，54，： | 1 |
| 6 | Activin A and follistatin in systemic inflammation显示文摘 | Kristian LJ David MK David JP | 2004 | Mol Cell Endocrinol2004，225，12： | 1 |
| 7 | Differential effect of acut baroreceptor unloading on cardiac and systemic sympathetic tone in congestive hart failure显示文摘 | David MK Garry LJ Anthony MD | 1998 | JACC1998，31，： | 1 |
| 8 | Utility of 12-lead electrocardiogram in localizing the origin of right ventricular outflow tract tachycardia 显示文摘 | David SS Mark WP | 1995 | Am Heart J1995，130，： | 1 |
| 9 | Functional agonism of insect odorant receptor ion channels 显示文摘 | Patrick LJ Gregory MP David C | 2011 | PNAS2011，108，21： | 1 |
| 10 | 3D Conformal radiotherapy for gastric cancer-results of a comparative planning study显示文摘 | Trevor L David W Paryl LJ | 2005 | Radiother Oncol2005，74，3： | 1 |
| 11 | Male gender is an independent risk factor for major infection after severe blunt trauma 显示文摘 | Jeffrey LJ Ernest M David C | 2004 | J Am Coll Surg2004，199，3： | 1 |
| 12 | A human lymphocyte homing receptor, the hermes antigen, is related to cartilage proteoglycan core and link protein显示文摘 | Goldstein LA David FH Pocker LJ | 1998 | Cell1998，56，： | 1 |
| 13 | Dynameomics: Mass annotation of protein dynamics and unfolding in water by high-throughput atomistic molecular dynamics simulations显示文摘 | DAVID A C B AMANDA LJ SCHAEFFER R D | 2008 | Protein Eng Des Sel2008，21，6： | 1 |
| 14 | Ultrasonography and developmental dysplasia of the hip:a cost-benefit analysis of three delivery systems显示文摘 | Davids JR Benson LJ Mubarak SJ | 1995 | J Pediatr Orthop1995，15，3： | 1 |
| 15 | Vascular endothelial growth factor: Direct neuroprotective effect in in vitro ischemia显示文摘 | Kun LJ Xiao OM David A | 2000 | Proc Natl Acad Sci USA2000，97，10： | 1 |
| 16 | Chemistry of hypusine formation on eukaryotic initiation factor 5A in biology systems显示文摘 | David LJ | 1999 | J Chin Chem Soc1999，46，5： | 1 |
| 17 | Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis 显示文摘 | Matthew LJ Mark OC David G | 2012 | Cell Host Microb2012，6，5： | 1 |
| 18 | The glycemic index: methodology and clinical implication显示文摘 | Wolever TMS David JA Alexandra LJ | 1991 | Am J Clin Nutr1991，54，： | 1 |
| 19 | Contemporary approaches for imaging skeletal metastasis显示文摘The skeleton is a common site of cancer metastasis. Notably high incidences of bone lesions are found for breast, prostate, and renal carcinoma. Malignant bone tumors result in significant patient morbidity.Identification of these lesions is a critical step to accurately stratify patients, guide treatment course, monitor disease progression, and evaluate response to therapy. Diagnosis of cancer in the skeleton typically relies on indirect bone-targeted radiotracer uptake at sites of active bone remodeling. In this manuscript, we discuss established and emerging tools and techniques for detection of bone lesions, quantification of skeletal tumor burden, and current clinical challenges. | David Ulmert Lilja Solnes Daniel LJ Thorek | 2015 | Bone Research2015，3，2： | 0 |
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