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您的检索式：作者名="David S.Roberts"
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| 1 | Reproducible large-scale synthesis of surface silanized nanoparticles as an enabling nanoproteomics platform: Enrichment of the human heart phosphoproteome显示文摘A reproducible synthetic strategy was developed for facile large-scale (200 mg) synthesis of surface silanized magnetite (Fe3O4) nanoparticles (NPs) for biological applications.After further coupling a phosphate-specific affinity ligand,these functionalized magnetic NPs were used for the highly specific enrichment of phosphoproteins from a complex biological mixture.Moreover,correlating the surface silane density of the silanized magnetite NPs to their resultant enrichment performance established a simple and reliable quality assurance control to ensure reproducible synthesis of these NPs routinely in large scale and optimal phosphoprotein enrichment performance from batch-to-batch.Furthermore,by successful exploitation of a top-down phosphoproteomics strategy that integrates this high throughput nanoproteomics platform with online liquid chromatography (LC) and tandem mass spectrometry (MS/MS),we were able to specifically enrich,identify,and characterize endogenous phosphoproteins from highly complex human cardiac tissue homogenate.This nanoproteomics platform possesses a unique combination of scalability,specificity,reproducibility,and efficiency for the capture and enrichment of low abundance proteins in general,thereby enabling downstream proteomics applications. | David S.Roberts Bifan Chen Timothy N.Tiambeng Zhijie Wu Ying Ge Song Jin | 2019 | Nano Research2019，12，6： | 1 |
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