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| 1 | Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45显示文摘Objective:Side population(SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer.Many kinds of cell lines and tissues have demonstrated the presence of SP cells,including several gastric cancer cell lines.This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45.Methods:We used fluorescence activated cell sorting(FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45(cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells.Results:This study found that the SP cells had higher clone formation efficiency than major population(MP) cells.Five stemness-related gene expression profiles,including OCT-4,SOX-2,NANOG,CD44,and adenosine triphosphate(ATP)-binding cassette transporters gene ABCG2,were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction(RT-PCR).Western blot was used to show the difference of protein expression between SP and MP cells.Both results show that there was significantly higher protein expression in SP cells than in MP cells.When inoculated into non-obese diabetic/severe combined immunodeficiency(NOD/SCID) mice,SP cells show higher tumorigenesis tendency than MP cells.Conclusions:These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells. | Hai-hong ZHANG Ai-zhen CA Xue-ming WEI Li DING Feng-zhi LI Ai-ming ZHENG Da-jiang DAI Rong-rong HUANG Hou-jun CAO Hai-yang ZHOU Jian-mei WANG Xue-jing WANG Wei SHI Heng ZHU Xiao-ying YUAN Lin CHEN | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，3： | 9 |
| 2 | Saturated fatty acid induction of endoplasmic reticulum stress and apopto- sis in human liver cells via the PERK/ATF4/CHOP signaling pathway显示文摘 | CA() 3 DAI DL YAO L | 2012 | Mol Cell Biochem2012，364，12： | 1 |
| 3 | Development and evaluation of 50-mer oligonucleotide arrays for detecting microbial populations in Acid Mine Drainages and bioleaching systems 显示文摘 | YIN H CA L QIU G WANG D KELLOGG L ZHOU J DAI Z LIU X | 2007 | J Microbiol Meth2007，70，： | 1 |
| 4 | A structural equation model relating physical function, pain, impaired mobility (1M), and falls in older adults显示文摘 | Dai B Ware WB Giuliani CA | 2012 | Arch Gerontol Geriatr2012，55，3： | 1 |
| 5 | A structural equation model relating physical function, pain, impaired mobility (1M), and falls in older adults显示文摘 | Dai B Ware WB Giuliani CA | 2012 | Arch Gerontol Geriatr2012，55，3： | 1 |
| 6 | The Common Land Model 显示文摘 | Dai Y Zeng X Dickinson RE Baker I Bonan GB Bosilovich MG Denning AS Dirmeyer PA Houser PR Niu G Oleson KW Schlosser CA Yang Z-L | | Bulletin of the American Meteorological Society0，84，： | 1 |
| 7 | Matrix - assisted laser desorption ionization mass spectrometry for the analysis of monosulfated oligosaccharides显示文摘 | Dai Y Whittal RM Bridges CA | 1997 | Carbohydr Res1997，304，： | 1 |
| 8 | Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species显示文摘 | Ca(n)as J E Long M Nations S Vadan R Dai L Luo M Ambikapathi R Lee E H Olszyk D | | 0，，09： | 1 |
| 9 | The TAF250 subunit of TFⅡD has histoneacetyltransferase activity显示文摘 | Mizzen CA Yang Y Dai P | 1996 | Cell1996，87，： | 1 |
| 10 | Pediatric central corneal thickness variation among major ethnic populations 显示文摘 | Dai E Gunderson CA | 2006 | JAAPOS2006，10，1： | 1 |
| 11 | Epidermal growth factor receptor localization in the rat and monkey testes 显示文摘 | Suatez Quian CA Dai MZ Onoda M | 1989 | Biol Reprod1989，41，5： | 1 |
| 12 | Pediatric central corneal thickness variation among major ethnic population 显示文摘 | Dai E Gunderson CA | 2006 | J AAPOS2006，10，1： | 1 |
| 13 | Matrix-assisted Laser Desorption Ionazation Mass Spectrometry for the Analysis of Monosulfated Oligosaccharides显示文摘 | Dai Y Whittal RM Bridges CA | 1997 | Carbohydrate Res1997，304，1： | 1 |
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