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| 1 | Three-dimensional biofabrication of an aragoniteenriched self-hardening bone graft substitute and assessment of its osteogenicity in vitro and in vivo显示文摘A self-hardening three-dimensional(3D)-porous composite bone graft consisting of 65 wt%hydroxyapatite(HA)and 35 wt%aragonite was fabricated using a 3D-Bioplotter®.New tetracalcium phosphate and dicalcium phosphate anhydrous/aragonite/gelatine paste formulae were developed to overcome the phase separation of the liquid and solid components.The mechanical properties,porosity,height and width stability of the end products were optimised through a systematic analysis of the fabrication processing parameters including printing pressure,printing speed and distance between strands.The resulting 3D-printed bone graft was confirmed to be a mixture of HA and aragonite by X-ray diffraction,Fourier transform infrared spectroscopy and energy dispersive X-ray spectroscopy.The compression strength of HA/aragonite was between 0.56 and 2.49 MPa.Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay in vitro.The osteogenicity of HA/aragonite was evaluated in vitro by alkaline phosphatase assay using human umbilical cord matrix mesenchymal stem cells,and in vivo by juxtapositional implantation between the tibia and the anterior tibialis muscle in rats.The results showed that the scaffold was not toxic and supported osteogenic differentiation in vitro.HA/aragonite stimulated new bone formation that bridged host bone and intramuscular implants in vivo.We conclude that HA/aragonite is a biodegradable and conductive bone formation biomaterial that stimulates bone regeneration.Since this material is formed near 37°C,it will have great potential for incorporating bioactive molecules to suit personalised application;however,further study of its biodegradation and osteogenic capacity is warranted.The study was approved by the Animal Ethical Committee at Tongji Medical School,Huazhong University of Science and Technology(IACUC No.738)on October 1,2017. | Yunsong Shi Ruijun He Xiangyu Deng Zengwu Shao Davide Deganello Chunze Yan Zhidao Xia | 2020 | Biomaterials Translational2020，1，1： | 2 |
| 2 | Infrahyoid fascio- myocutaneous flap as an alterative to free radial forearm flap in head and neck reconstruction显示文摘 | Deganello A Manciocco V Dolivet G | 2007 | Head Neck2007，29，3： | 1 |
| 3 | Nucleation,adhesion,and inter-nalization of calcium-containing urinary crystals by renal cells显示文摘 | Lieske JC Deganello S | | 0，，14： | 1 |
| 4 | Con- trast enhanced ultrasound (CEUS) characterization of grey-scale sonographic indetemlinate focal liver lesions in pediatric practice 显示文摘 | JACOB J DEGANELLO A SELLARS M E | 2013 | Ultraschall Med2013，34，6： | 1 |
| 5 | Influence of barium and cerium oxides on alumina supported Pd catalysts for hydrocarbon combustion显示文摘 | LIOTTA L F DEGANELLO G SANNINO D | 2002 | Appl Catal A:General2002，229，： | 1 |
| 6 | Phase analysis and oxygen storage capacity of ceria-lanthana-based TWC promoters prepared h、sol-gel routes显示文摘 | DEGANELLO F MARTORANA A | 2002 | Journal of Solid State Chemistr)2002，163，： | 1 |
| 7 | Potential complications in the use of wheat bran for constipation in infancy显示文摘 | Zoppi G Gobio-Casali L Deganello A | 1982 | J Pediatr Gastroenterol Nutr1982，1，： | 1 |
| 8 | Synergetic effect of gold in Au/Pd catalysts during hydrodesulfurization reactions of model compounds 显示文摘 | Venezia A M Parola V La Deganello G | 2003 | J Catal2003，215，： | 1 |
| 9 | Liquid phase hydrogenation of phenylacetylene on pumice supported palladium catalyst显示文摘 | Duca D Liotta L F Deganello G | 1995 | Catalysis Today1995，24，： | 1 |
| 10 | Subtotal su- pracricoid laryngectomy; changing in indications, surgical tech- niques and use of new surgical devices显示文摘 | Mannelli G Meccariello G Deganello A | 2014 | Am J Otolaryngol2014，35，6： | 1 |
| 11 | Citrate-nitrate autocombustion synthesis of perovskite-type nanopowders:A systematic approach显示文摘 | Deganello F Marci G Deganello G | 2009 | J Eur Ceram Soc2009，29，3： | 1 |
| 12 | Co_3O_4/CeO_2 and Co_3O_4/CeO_2-ZrO_2 composite catalysts for methane combustion:Correlation between morphology reduction properties and catalytic activity显示文摘 | Liotta L F Carlo G D Pantaleo G Deganello G | | 0，，： | 1 |
| 13 | Catalytic reduction of nitrates and nitrites in water solution on pumice-supported Pd- Cu catalysts显示文摘 | Deganello F Liotta L F Macaluso A | 2000 | Applied Catalysis B: Environmental2000，24，： | 1 |
| 14 | Ultrafast near-infrared sintering of a slot-die coated nano-silver conducting ink显示文摘 | Cherrington M Claypole T C Deganello D Mabbett I Watson T Worsley D | 2011 | Journal of Materials Chemistry2011，21，21： | 1 |
| 15 | Effectiveness and pitfalls of elective neck dissection in N0 laryngeal cancer显示文摘 | Deganello A Gitti G Meccariello G | 2011 | Acta Otorhinolaryngol Ital2011，31，4： | 1 |
| 16 | Contrast enhanced ultrasound(CEUS)characterization of grey-scale sonographic indeterminate focal liver lesions in pediatric practice显示文摘 | Jacob J Deganello A Sellars ME | 2013 | Ultraschall Med2013，34，6： | 1 |
| 17 | Thermal stability,structural properties and catalytic activity of Pd catalysts supported on Al2O3-CeO2-BaO mixed oxides prepared by sol-gel method显示文摘 | Liotta L F Deganello G | 2003 | Journal of Molecular Catalysis A:Chemical2003，，： | 1 |
| 18 | Palladium on pumice: new catalysts for the stereoselective semihydrogenation of alkynes to (Z)-alkenes显示文摘 | Gruttadauria M Liotta L F Noto R Deganello G | 2001 | Tetrahedron Letters2001，42，10： | 1 |
| 19 | Catalytic CO oxidation over pumice supported Pd-Ag catalysts显示文摘 | Venezia A M Liotta L F Deganello G Schay Z Horvath D Guczi L | 2001 | Applied Catalysis A: General2001，211，2： | 1 |
| 20 | Activity of SiO2 supported gold-palladium catalysts in CO oxidation 显示文摘 | Venezia A M Liotta L F Pantaleo G Parola V La Deganello G Beck A Kopp6ny Zs Frey K Horvath D Guczi L | 2003 | Applied Catalysis A: General2003，251，： | 1 |
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