|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A new semi-rigid implant for instrumentation of scoliosis :preliminary report 显示文摘 | Pfeiffer M Deike B Clausen JD | 2001 | Eur Spine J2001，10，5： | 1 |
| 2 | Association of overall survival in patients with newly diagnosed glioblastoma with contrast-enhanced perfusion MRI: Comparison of intraindividually matched T1- and T*2 -based bolus techniques显示文摘 | Bonekamp D Deike K Wiestler B | 2014 | J Magn Reson Imaging2014，，： | 1 |
| 3 | Exact focusing of extracorporeal shock wave therapy for calcifying tendinopathy显示文摘 | HAAKE M DEIKE B THON A | 2002 | Clin Orthop Relat Res2002，，397： | 1 |
| 4 | Detection of liver metastases: Comparison of contrast-enhanced wide-band harmonic imaging with conventional ultrasongraphy显示文摘 | Thomas B Deike S Eckhart GH | 2001 | J Ultrasound Med2001，20，： | 1 |
| 5 | A new in vitro mod- el of the glial scar inhibits axon growth显示文摘 | Wanner I B Deik A Torres M | 2008 | Glia2008，56，15： | 1 |
| 6 | Investigations on the energy efficiency of organic and integrated fanning with specific emphasis on pesticide use intensity显示文摘 | Deike S Pallutt B Christen O | 2008 | European Journal of Agronomy2008，28，： | 1 |
| 7 | A geochemical hypothesis for dolomitization by groundwater 显示文摘 | HANSHAW B B BLACK W DEIKE R G | 1971 | Economic Geology1971，，： | 1 |
| 8 | A geochemical hypothesis of dolomitization by ground water显示文摘 | Hanshow B B Back W Deike R G | 1971 | Economy Geology1971，66，： | 1 |
| 9 | A geochemical hypothesis of dolomitization by ground water显示文摘 | Hanshow B B Back W Deike R G | 1971 | Economy Geology1971，66，： | 1 |
| 10 | Long-term productivity and environmental effects of arable farming as affected by crop rotation, soil tillage intensity and strategy of pesticide use: A case-study of two long-term field experiments in Germany and Denmark 显示文摘 | Deike S Pallutt B Melander B | 2008 | Eur J Agron2008，29，4： | 1 |
| 11 | Exact focusing of extracorporeal shock wave therapy for calcifying tendinopathy显示文摘 | Haake M Deike B Thon A | 2002 | Clin Orthop Relat Res2002，397，： | 1 |
| 12 | Detection of liver metastases :comparison of contrast-enhanced wide-band harmonic imaging with conventional uhrasonography 显示文摘 | homas B Deike S Eckhart GH | 2001 | Ultrasound Med2001，20，： | 1 |
| 13 | A geochemical hypothesis of dolomitization by ground water 显示文摘 | Hanshow B B Back W Deike R G | 1971 | Econ Geol1971，66，： | 1 |
| 14 | A new in vitro model of the glial scar inhibits axon growth显示文摘 | Wanner I B Deik A Torres M | 2008 | Glia2008，56，15： | 1 |
| 15 | Exact focusing of extracorporeal shock wave therapy for calcifying tendinopathy 显示文摘 | HaakeM Deike B Thon A | 2002 | Clin Orthop2002，397，： | 1 |
| 16 | Apolipoprotein B100 is required for hepatitis C infectivity and Mipomersen inhibits hepatitis C显示文摘AIM To characterize the role of apolipoprotein B100(apoB 100) in hepatitis C viral(HCV) infection. METHODS In this study, we utilize a gene editing tool, transcription activator-like effector nucleases(TALENs), to generate human hepatoma cells with a stable genetic deletion of APOB to assess of apoB in HCV. Using infectious cell culture-competent HCV, viral pseudoparticles, replicon models, and lipidomic analysis we determined the contribution of apoB to each step of the viral lifecycle. We further studied the effect of mipomersen, an FDAapproved antisense inhibitor of apoB 100, on HCV using in vitro cell-culture competent HCV and determined itsimpact on viral infectivity with the TCID50 method. RESULTS We found that apo B100 is indispensable for HCV infection. Using the JFH-1 fully infectious cell-culture competent virus in Huh 7 hepatoma cells with TALENmediated gene deletion of apoB(APOB KO), we found a significant reduction in HCV RNA and protein levels following infection. Pseudoparticle and replicon models demonstrated that apo B did not play a role in HCV entry or replication. However, the virus produced by APOB KO cells had significantly diminished infectivity as measured by the TCID-50 method compared to wildtype virus. Lipidomic analysis demonstrated that these virions have a fundamentally altered lipidome, with complete depletion of cholesterol esters. We further demonstrate that inhibition of apoB using mipomersen, an FDA-approved anti-sense oligonucleotide, results in a potent anti-HCV effect and significantly reduces the infectivity of the virus. CONCLUSION Apo B is required for the generation of fully infectious HCV virions, and inhibition of apo B with mipomersen blocks HCV. Targeting lipid metabolic pathways to impair viral infectivity represents a novel host targeted strategy to inhibit HCV. | Esperance AK Schaefer James Meixiong Christina Mark Amy Deik Daniel L Motola Dahlene Fusco Andrew Yang Cynthia Brisac Shadi Salloum Wenyu Lin Clary B Clish Lee F Peng Raymond T Chung | 2016 | World Journal of Gastroenterology2016，22，45： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员