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| 1 | The research progress OF Walnut nutrition medicinal value and processing technology 显示文摘 | LU Bin NING Delu BAO Jiangshan | 2006 | Fruit and Vegetable Processing2006，，4： | 1 |
| 2 | Optimization of ISSR-PCR Reaction System and Primer Selection for Olea euyopaea显示文摘[Objective] This study aimed to optimize the ISSR-PCR reaction system and select polymorphic ISSR primers for Olea euyopaea. [Method] O. euyopaea genomic DNA was extracted from leaves as the template for optimization of ISSR-PCR reaction system by single-factor experiments on the main factors including Mg2+, dNTPs, primer concentration and template amount. [Result] The optimal ISSR-PCR reaction system for O. euyopaea was obtained, with a total system volume of 20 μl containing 1 × Taq buffer, 3.5 mmol/L Mg2+, 0.4 mmol/L dNTPs, 1.0 μmol/L primers, 1.0 U of Taq DNA polymerase and 20 ng of DNA template. The optimal ISSR-PCR reaction program was started with predenaturation at 94 ℃ for 5 min, followed by 40 cycles of denaturation at 94 ℃ for 30 s, annealing at 52-55 ℃ for 30 s, and extension at 72 ℃ for 2 min; the amplification was completed by holding the reaction mixture at 72 ℃ for 10 min to allow complete extension of PCR products. PCR products were stored at 4 ℃. Based on the above conditions, 11 primers with high polymorphism, clear amplified bands and good repeatability were selected. [Conclusion] This study laid the foundation for further diversity research and species identification of O. euyopaea germplasm resources. | Rui LI Shaoyu CHEN Delu NING Yongjie LI Yunling MAO | 2012 | Agricultural Biotechnology2012，1，2： | 0 |
| 3 | Analysis of Climatic Factors Influencing Walnut Distribution in the Main Producing Areas of Yunnan Province显示文摘Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show that Yunnan walnut has a great expectation on temperature and moisture.Temperature,including average temperature in January,average temperature in July,the average annual temperature and accumulated temperature≥10℃,has the greatest impact on the distribution of Yunnan walnut,and is the primary dominant factor;moisture,including annual rainfall and average relative humidity,has a great impact on the distribution of Yunnan walnut,and is the secondary dominant factor. | Liangjun XIAO Ting MA Delu Ning | 2013 | Asian Agricultural Research2013，5，11： | 0 |
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