| 1 | Investigation of Matrix Metalloproteinase -1,2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis显示文摘Objective: To explore the role of matrix metalloproteinase-1,2(MMP-1, MMP-2) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in endometriosis. Methods: The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular endometria from 40 subjects(excluding endometriosis) were collected and examined by in situ hybridization technology and western blot assay. Results: Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant(P < 0.01). Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion: The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis. | Rong Liu Demin Pu Dan Yang | 2007 | Journal of Nanjing Medical University2007，21，3： | 1 |
| 2 | Clinical significance of survivin in the diagnosis and prognosis of endometrial carcinoma显示文摘Objective:To investigate the clinical significance of survivin in endometrial carcinoma and to investigate the relationship between the expression of survivin and Ki-67.Methods:Immunohistochemical S-P(streptavidin-biotin-peroxidase complex)method was performed to detect the expression of survivin and Ki-67 antigen in 15 cases of normal endometrium,21 cases of endometrial simple and complex hyperplasia,22 cases of endometrial atypical hyperplasia,and 61 cases of endometrial carcinoma.Results:Survivin was hardly detected in some normal endometrium in the proliferative phase and in the secretory phase.However,the level of survivin expression in atypical hyperplasia endometrium(72.73%)was higher than that in normal endometrium(7.14%)(P < 0.05),including simple and complex hyperplasia(42.38%)(P < 0.01),and was lower than that in endometrial carcinoma(90.17%)(P < 0.05).Moreover,significant correlation was present between the expression of survivin and the characteristics of endometrial carcinoma,including clinical stage,histological grade and the presence of invasion to myometrium(P < 0.05).In addition,Ki-67 antigen expression was positively correlated with survivin expression in all specimen.Ki-67 labeled indexes(LIs)in hyperplasia endometrium were significantly lower than those in atypical hyperplasia endometrium and endometrial carcinoma(P < 0.01),while there was no significant difference in Ki-67 LIs between atypical hyperplasia endometrium and endometrial carcinoma(P > 0.05).There was no significant relationship between Ki-67 LIs and the characteristics of endometrial carcinoma,including histological grade,clinical stage or the invasion to myometrium(P > 0.05).Conclusion:Survivin may participate in the onset and progression of endometrial carcinoma through inhibiting apoptosis and promoting proliferation.Survivin expression is correlated with the malignant degree and prognosis of tumor.Ki-67 is also associated with carcinogenesis and progression of endometrial carcinoma.The results suggest that survivin could be a diagnostic and prognostic marker for endometrial carcinoma and might provide pathways to treat the patients with recurrent or refractory or rudimental endometrial carcinoma. | Yanxiang Cheng Gantao Chen Yanjun Cheng Demin Pu | 2006 | Journal of Nanjing Medical University2006，20，4： | 0 |
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