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| 1 | Effects of Lactobacillus plantarum and fibrolytic enzyme on the fermentation quality and in vitro digestibility of total mixed rations silage including rape straw显示文摘The aim of the current study was to investigate the effects of a lactic acid bacteria inoculant(Lactobacillus plantarum, LP), fibrolytic enzyme(EN), combination of LP and EN(LP+EN) on fermentation quality, nutritive characteristics and in vitro digestibility of total mixed ration(TMR) silages containing 0, 7.5 and 15.0%(on dry matter basis) of rape(Brassica campestris L.) straw(RS)(denoted as CTMR, LTMR and MTMR, respectively). After ensiling for 60 days, TMR silages without additives were well preserved, but MTMR had higher p H than CTMR and LTMR. There were no differences in other parameters of fermentation quality, microbial composition, nutrition and in vitro digestibility between CTMR and LTMR except for yeast and mold number and crude protein(CP) content. CTMR and LTMR silage had higher CP content, in vitro neutral detergent fiber digestibility(IVNDFD) and in vitro acid detergent fiber digestibility(IVADFD), lower acid detergent fiber(ADF) content than MTMR silage. LP and EN decreased p H and increased dry matter(DM) recovery of TMR silages. LP+EN improved the fermentation quality, nutritive characteristics and in vitro digestibility of TMR silages, showed by lower p H, ADF content, higher lactic acid content, in vitro dry matter digestibility(IVDMD) and IVNDFD. Therefore, It was suggested that TMR silage contained 7.5% RS on a DM basis and treated with LP+EN can be as a useful feed for ruminant. | LIU Qin-hua LI Xiang-yu Seare T Desta ZHANG Jian-guo SHAO Tao | 2016 | Journal of Integrative Agriculture2016，15，9： | 27 |
| 2 | 西藏地区牦牛瘤胃中兼性厌氧纤维素降解菌的分离鉴定显示文摘本研究旨在从西藏地区牦牛瘤胃中分离出具有分解纤维素能力的兼性厌氧菌,为青贮饲料微生物添加剂的研制提供支持。试验采集了西藏那曲地区成年牦牛的新鲜瘤胃液,经刚果红染色初筛,滤纸降解复筛,获得分解纤维素能力强的菌株,对菌株生长速率及酶活力进行测定,经形态学、生理生化特性和16SrRNA序列分析对菌株进行鉴定,并分析了菌株对水稻秸秆的降解效果。试验分离出一株兼性厌氧纤维素降解菌,属于肠球菌属,即粪肠球菌(Enterococcus faecalis)JF85。菌株JF85在15~55℃,pH 3.0~7.0及3.0%和6.5%NaCl培养基中生长良好。接种JF85,36h后可获得最大内切葡聚糖酶活力(0.41U/mL)和滤纸酶活力(0.13U/mL)。在模拟发酵试验中,接种菌株JF85,14d后水稻秸秆干物质、纤维素和半纤维素含量与对照组相比显著降低(P<0.05);在发酵第7天,JF85处理组显示最高的可溶性碳水化合物含量,整个发酵过程中木质素含量变化不显著(P>0.05)。菌株JF85具有分解纤维素、耐酸、耐盐特性,在青贮饲料生产中具有较好的应用前景。 | 李君风 原现军 董志浩 Seare Tajebe Desta 陈雷 白晰 白云峰 邵涛 | 2017 | 草业学报2017，26，6： | 12 |
| 3 | Maternal and fetal outcomes in term premature rupture of membrane显示文摘BACKGROUND: Premature rupture of membrane(PROM) is linked to significant maternal prenatal mortalities and morbidity. In Ethiopia, where maternal mortality is still high, the maternal and fetal outcomes in PROM is very important to decrease maternal and child mortality and for better management and prevention of complications. Thus, this study aimed to detect the maternal and fetal outcomes and associated factors in term PROM at Mizan-Aman General Hospital, south-west Ethiopia.METHODS: A retrospective cross sectional study was conducted using data available at MizanAman General Hospital during a period of 3 years(January 2011 to December 2013). We examined records of 4 525 women who gave birth in the hospital; out of these women, 185 were diagnosed with term PROM and all of them were included in the study. The data of these women were collected using a checklist based on registration books. The data were analyzed using SPSS version 20.0 statistical package. The association between independent and dependent variables was assessed by bivariate and multiple logistic regression analyses. 95%CI and P value less than 0.05 were considered statistically signifi cant.RESULTS: Of the 4 525 women who gave birth in the hospital, 202 were complicated by term PROM. About 22.2% of the women showed unfavorable maternal outcomes. The most common cause of maternal morbidity and mortality was puerperal sepsis. About 33.5% of neonates experienced unfavorable outcomes. The duration of PROM >12 hours(AOR=5.6, 95%CI 1.3–24.1) latency >24 hours(AOR=2.8, 95%CI 1.7–11.8), residing in rural areas(AOR=4.2, 95%CI 3.96–29.4) and birth weight less than 2 500 g were associated with unfavorable outcomes.CONCLUSION: Women residing in rural areas, long latency, and neonates with birth weight less 2 500 g may have unfavorable outcomes. Therefore, optimum obstetric and medical care is essential for the reduction of the devastating complications related to disorders. | Tigist Endale Netsanet Fentahun Desta Gemada Mamusha Aman Hussen | 2016 | World Journal of Emergency Medicine2016，7，2： | 6 |
| 4 | The incidence of oxygen desaturation during rapid sequence induction and intubation显示文摘BACKGROUND: Rapid sequence induction and intubation(RSII) is an emergency airway management technique for patients with a risk of pulmonary aspiration. It involves preoxygenation, administration of predetermined doses of induction and paralytic drugs, avoidance of mask ventilation, and laryngoscopy followed by tracheal intubation and keeping cricoid pressure applied till endotracheal tube cuff be inflated. Oxygen desaturation has been seen during RSII. We assessed the incidence of oxygen desaturation during RSII.METHODS: An institution-based observational study was conducted from March 3 to May 4, 2014 in our hospital. All patients who were operated upon under general anesthesia with RSII during the study period were included. A checklist was prepared for data collection.RESULTS: A total of 153 patients were included in this study with a response rate of 91.6%. Appropriate drugs for RSII, equipments for RSII, equipments for diffi cult intubation, suction machine with a catheter, a monitor and an oxygen backup such as ambu bag were not prepared for 41(26.8%), 50(32.7%), 51(33.3%), 38(24.8%) and 25(16.3%) patients respectively. Cricoid pressure was not applied at all for 17(11.1%) patients and 53(34.6%) patients were ventilated after induction of anesthesia but before intubation and endotracheal cuff inflation. A total of 55(35.9%) patients desaturated during RSII(SPO2<95%). The minimum, maximum and mean oxygen desaturations were 26%, 94% and 70.9% respectively. The oxygen desaturation was in the range of <50%, 50%–64%, 65%–74%, 75%–84%, 85%–89 % and 90%–94% for 6(3.9%), 7(4.6%), 5(3.3%), 10(6.5%), 13(8.5%) and 14(9.2%) patients respectively.CONCLUSION: The incidence of oxygen desaturation during RSII was high in our hospital. Preoperative patient optimization and training about the techniques of RSII should be emphasized. | Endale Gebreegziabher Gebremedhn Desta Mesele Derso Aemero Ehtemariam Alemu | 2014 | World Journal of Emergency Medicine2014，5，4： | 5 |
| 5 | β-1,4-葡聚糖内切酶基因在大肠杆菌中分泌表达显示文摘采用重叠延伸PCR合成技术将乳酸乳球菌(Lactococcus lactis)分泌蛋白基因序列usp45(81 bp)和瑞氏木霉(Trichoderma reesei)β-1,4-葡聚糖内切酶成熟蛋白序列egl3(657 bp)连接成融合基因usp45-egl3,构建了分泌型重组质粒pMG36e-usp45-egl3及重组大肠杆菌E.coli DH5α/pMG36e-usp45-egl3。分析重组大肠杆菌的表达效果及表达产物降解纤维素的能力。结果表明,重组大肠杆菌DH5α/pMG36e-usp45-egl3经14 h培养能分泌酶活为226 mU/mL的β-1,4-葡聚糖内切酶,并有效地降解了羧甲基纤维素钠。其中分泌的β-1,4-葡聚糖内切酶降解羧甲基纤维素钠生成还原糖的速率为2.35 mg/(h·mL)。 | 李湘玉 刘秦华 李君风 白晰 T. Desta 王思然 董志浩 白云峰 邵涛 | 2018 | 食品与生物技术学报2018，37，3： | 4 |
| 6 | Asymmetric photocurrent extraction in semitransparent laminated flexible organic solar cells显示文摘Scalable production methods and low-cost materials with low embodied energy are key to success for organic solar cells.PEDOT(PSS)electrodes meet these criteria and allow for low-cost and all solution-processed solar cells.However,such devices are prone to shunting.In this work we introduce a roll-to-roll lamination method to construct semitransparent solar cells with a PEDOT(PSS)anode and an polyethyleneimine(PEI)modified PEDOT(PSS)cathode.We use the polymer:PCBM active layer coated on the electrodes as the lamination adhesive.Our lamination method efficiently eliminates any shunting.Extended exposure to ambient degrades the laminated devices,which manifests in a significantly reduced photocurrent extraction when the device is illuminated through the anode,despite the fact that the PEDOT(PSS)electrodes are optically equivalent.We show that degradation-induced electron traps lead to increased trap-assisted recombination at the anode side of the device.By limiting the exposure time to ambient during production,degradation is significantly reduced.We show that lamination using the active layer as the adhesive can result in device performance equal to that of conventional sequential coating. | Jonas Bergqvist Thomas Osterberg Armantas Melianas Luis Ever Aguirre Zheng Tang Wanzhu Cai Zaifei Ma Martijn Kemerink Desta Gedefaw Mats R.Andersson Olle InganAs | 2018 | npj Flexible Electronics2018，2，1： | 4 |
| 7 | Accuracy assessment of real-time kinematics(RTK)measurements on unmanned aerial vehicles(UAV)for direct geo-referencing显示文摘Geospatial information acquired with Unmanned Aerial Vehicles(UAV)provides valuable decision-making support in many different domains,and technological advances coincide with a demand for ever more sophisticated data products.One consequence is a research and development focus on more accurately referenced images and derivatives,which has long been a weakness especially of low to medium cost UAV systems equipped with relatively inexpensive inertial measurement unit(IMU)and Global Navigation Satellite System(GNSS)receivers.This research evaluates the positional accuracy of the real-time kinematics(RTK)GNSS on the DJI Matrice 600 Pro,one of the first available and widely used UAVs with potentially surveying-grade performance.Although a very high positional accuracy of the drone itself of 2 to 3 cm is claimed by DJI,the actual accuracy of the drone RTK for positioning the images and for using it for mapping purposes without additional ground control is not known.To begin with,the actual GNSS RTK position of reference center(the physical point on the antenna)on the drone is not indicated,and uncertainty regarding this also exists among the professional user community.In this study the reference center was determined through a set of experiments using the dual frequency static Leica GNSS with RTK capability.The RTK positioning data from the drone were then used for direct georeferencing,and its results were evaluated.Test flights were carried out over a 70 x 70 m area with an altitude of 40 m above the ground,with a ground sampling distance of 1.3 cm.Evaluated against ground control points,the planimetric accuracy of direct georeferencing for the photogrammetric product ranged between 30 and 60 cm.Analysis of direct georeferencing results showed a time delay of up to 0.28 seconds between the drone GNSS RTK and camera image acquisition affecting direct georeferencing results. | Desta Ekaso Francesco Nex Norman Kerle | 2020 | Geo-Spatial Information Science2020，23，2： | 3 |
| 8 | 以蛋白质-蛋白质相互作用为靶点的HIV抑制剂的研究进展显示文摘蛋白质-蛋白质相互作用(protein-protein interaction, PPI)在人免疫缺陷病毒(human immunodeficiency virus, HIV)生命周期中的多个阶段发挥重要作用。针对这些相互作用特别是HIV与宿主细胞之间的相互作用为我们研发靶向于新结合位点和具有新作用机制的HIV抑制剂提供了新思路。因此本综述基于多种蛋白质-蛋白质相互作用的作用机制,结合具体研究实例阐述了该类抑制剂的最新研究进展。 | 汪昭 Desta SAMUEL 康东伟 展鹏 刘新泳 | 2019 | 药学学报2019，54，4： | 2 |
| 9 | Phlegmonous gastritis: case report and review显示文摘 | Gordon Y. Kim John Ward Bruce Henessey Joyce Peji Christopher Godell Hiwot Desta Scott Arlin John Tzagournis Fred Thomas | 2005 | Gastrointestinal Endoscopy2005，，1： | 2 |
| 10 | Ocular manifestation and their associated factors among HIV/AIDS patients receiving highly active antiretroviral therapy in Southern Ethiopia显示文摘AIM:To assess the pattern of ocular manifestation and associated factors among human immunodeficiency virus(HIV)/acquired immunodeficiency syndrome(AIDS) patients on highly active antiretroviral therapy(HAART) at Hawassa University Referral Hospital, Southern Ethiopia. METHODS:A cross sectional study was conducted from January 2014 to April 2015. After obtaining informed written consent, 240 adult HIV/AIDS patients on HAART were randomly selected regardless of their ophthalmic symptoms, WHO status or CD4 count. Data were collected using structured questionnaires and ophthalmologic clinical examination. Data were entered and analyzed using SPSS version 20.0 software. RESULTS:The mean duration of HAART was 62.5mo. The prevalence of HIV related ocular manifestation was 14.2%. Seborrheic blepharitis(5%) was the most common ocular manifestation, followed by squamoid conjunctival growth(3.8%). The rate of ocular manifestation was significantly higher among study participants who had CD4+ count <200 cells/μL(AOR=3.83; 95%CI:1.315-11.153), low duration of HAART(AOR=3.0; 95%CI:1.305-6.891) and who had primary school education [odds ratio(OR) =2.8; 95%CI:1.105-7.099]. Prevalence of visual impairment and blindness was 10.9% and 5.8%, respectively.CONCLUSION:HAART may be the reason for the decline in the prevalence of ocular manifestation in HIV/AIDS patients in the study area. Ophthalmologic screening of HIV/AIDS patients, especially those with CD4 counts of <200/μL cells and in the first five years of HAART followup is recommended to reduce visual impairment and/or blindness. | Anteneh Amsalu Kindie Desta Demiss Nigussie Demoze Delelegne | 2017 | International Journal of Ophthalmology(English edition)2017，10，5： | 2 |
| 11 | Comprehensive evaluation of tamoxifen sequential biotransformation by the human cytochrome P450system in vitro: prominent roles for CYP3A and CYP'2D6显示文摘 | Desta Z Ward BA Soukhova NV | 2004 | J Pharmacol Exp Ther2004，310，： | 1 |
| 12 | Diabetes enhances periodontal bone loss through enhanced resorption and diminished bone formation显示文摘 | Liu R Bal HS Desta T | 2006 | J Dent Res2006，85，6： | 1 |
| 13 | Diabetes-enhanced tumor necrosis factor-alpha production promotes apoptosis and the loss of retinal microvascular cells in type 1 and type 2 models of diabetic retinopathy显示文摘 | Behl Y Krothapalli P Desta T | | 0，，05： | 1 |
| 14 | Di-abetes-enhanced tumor necrosis factor-oproduction promotes apoptosis and the loss of retinal microvascular cells in type 1 and type 2 models of diabetic retinopathy显示文摘 | Behl Y Krothapalli P Desta T Dipiazza A Roy S Graves DT | 2008 | Am J Pathol2008，172，5： | 1 |
| 15 | Cytochrome P450 pharmacogenetics as a predictor of toxicity and clinical response to pulse cyclophosphamide in lupus nephritis显示文摘 | Takada K Arefayene M Desta Z | 2004 | Arthritis Rheum2004，50，7： | 1 |
| 16 | CYP2D6 genotype,antidepressant use,and tamoxifen metabolism during adjuvant breast cancer treatment显示文摘 | Jin Y Desta Z Steams V | 2005 | J Natl Cancer Inst2005，97，1： | 1 |
| 17 | Diabetes-enhanced tumor necrosis factor-alpha production promotes apoptosis and the loss of retinal microvascular cells in type 1 and type 2 models of diabetic retinopathy 显示文摘 | Behl Y Krothapalli P Desta T | 2008 | Am J Pathol2008，172，5： | 1 |
| 18 | Diabetes prolongs the inflam- matory response to a bacterial stimulus through cytokine dysregulation 显示文摘 | Naguib G A1-Mashat H Desta T | 2004 | J Invest Dermatol2004，123，1： | 1 |
| 19 | Impaired wound healing in mouse models of diabetes is mediated by TNF- c~dysregulation and associated with enhanced activation of forkhead box OI(FOXO1)显示文摘 | Siqueiraa MF Chehab L Desta T | 2010 | Diabetologia2010，58，2： | 1 |
| 20 | Quantitative effect of CYP2D6 genotype and inhibitors on tamoxifen metabolism:implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，1： | 1 |
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