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| 1 | Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides显示文摘Engineered scaffolds for bone tissue regeneration are designed to promote cell adhesion,growth,proliferation and differentiation.Recently,covalent and selective functionalization of glass and titanium surfaces with an adhesive peptide(HVP)mapped on[351e359]sequence of human Vitronectin allowed to selectively increase osteoblast attachment and adhesion strength in in vitro assays,and to promote osseointegration in in vivo studies.For the first time to our knowledge,in this study we investigated the resistance of adhesion sequences to proteolytic digestion:HVP was completely cleaved after 5 h.In order to overcome the enzymatic degradation of the native peptide under physiological conditions we synthetized three analogues of HVP sequence.A retro-inverted peptide D-2HVP,composed of D amino acids,was completely stable in serum-containing medium.In addition,glass surfaces functionalized with D-2HVP increased human osteoblast adhesion as compared to the native peptide and maintained deposition of calcium.Interestingly,D-2HVP increased expression of IBSP,VTN and SPP1 genes as compared to HVP functionalized surfaces.Total internal reflection fluorescence microscope analysis showed cells with numerous filopodia spread on D-2HVP-functionalized surfaces.Therefore,the D-2HVP sequence is proposed as new osteoblast adhesive peptide with increased bioactivity and high proteolytic resistance. | Annj Zamuner Paola Brun Michele Scorzeto Giuseppe Sica Ignazio Castagliuolo Monica Dettin | 2017 | Bioactive Materials2017，2，3： | 3 |
| 2 | Immortaliza-tion of mouse germ line stem cells显示文摘 | Hofmann M C Braydich-Stolle L Dettin L | 2005 | Stem Cells2005，23，2： | 1 |
| 3 | Generation and in vitro differentiation of a spermatogonial cell line显示文摘 | Feng LX Chen YL Dettin L | 2002 | Science2002，297，： | 1 |
| 4 | Morphological characterization of the spermatogonial subtypes in the neonatal mouse testis 显示文摘 | Dettin L Ravindranath N Hofmann MC | 2003 | Biol Reprod2003，69，5： | 1 |
| 5 | Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse显示文摘 | Nalbandian A Dettin L Dym M | 2003 | Boil Reprod2003，69，3： | 1 |
| 6 | Regulated expression and ultrastructural localization of galectin-1,a proapoptotic β-galactoside-binding lectin,during spermatogenesis in rat testis显示文摘 | Dettin L Rubinstein N Aoki A | 2003 | Biol Reprod2003，68，1： | 1 |
| 7 | Generation and in vitrodifferentiation of a spermatogonial cell line显示文摘 | Feng L X Chen Y L Dettin L | 2002 | Science2002，297，： | 1 |
| 8 | Generation and in vitro differentiation of a spermatogonial cell line显示文摘 | Feng L X Chen Y Dettin L | 2002 | Science2002，297，5580： | 1 |
| 9 | Facile and selective covalent grafting of an RGD-peptide to electrospun scaf- folds improves HUVEC adhesion显示文摘 | Dettin M Zamuner A Roso M | 2015 | J Pept Sci2015，21，10： | 1 |
| 10 | Generation and in vitro differentiation of a spermatogonial cell line显示文摘 | Yali Chen Dettin L | 2002 | Science2002，297，： | 1 |
| 11 | Immortalization of mouse germ line stem cells显示文摘 | Hofmann M C Braydich-Stolle L Dettin L | 2005 | Stem Calls2005，23，2： | 1 |
| 12 | Generation and in vitro differentiation of a spermatogonial cell line 显示文摘 | Feng LX Chen Y Dettin L | 2002 | Science2002，297，5580： | 1 |
| 13 | Desmoplakin is required for microvascular tube formation in culture显示文摘 | Zhou X Stuart A Dettin LE | 2004 | J Cell Sci2004，117，15： | 1 |
| 14 | In vitro and in vivo pro- angiogenic effects of thymosin-β4-derived peptides 显示文摘 | Dettin M Ghezzo F Coneoni MT | 2011 | Cell Immunol2011，271，2： | 1 |
| 15 | Id2 drives differentiation and suppresses tumor formation in the intestinal epithelium 显示文摘 | Russell RG Lasorella A Dettin LE | 2004 | Cancer Res2004，64，20： | 1 |
| 16 | Immortalization of mouse germ line stem cells 显示文摘 | Hofmann MC Braydich-Stolle L Dettin L | 2005 | Stem Cells2005，23，2： | 1 |
| 17 | Novel osteoblast-adhesive peptides for dental/orthopedic biomaterials显示文摘 | Dettin M Conconi MT Gambaretto R | 2002 | J Biomed Mater Res2002，60，3： | 1 |
| 18 | Generation and in vitro differentiation of a spermatogonial cell line显示文摘 | Chen Y Dettin L | 2002 | Science2002，297，5580： | 1 |
| 19 | Novel immobilizations of an adhesion peptide on the TiO2 surface:An XPS investigation显示文摘 | lucci G Dettin M Battocchio C | | 0，，： | 1 |
| 20 | Regulated expression and ultrastructural localization of galectin-1,a proapoptotic beta-galactoside-binding lectin,during spermato genesis in rat testis显示文摘 | Dettin L Rubinstein N Aoki A | | 0，，： | 1 |
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