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12篇 您的检索式：作者名="Dhungel A"

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		题名	作者	年代	出处	被引量
	1	Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC.	Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel	2017	World Journal of Stem Cells2017，9，9：	4
	2	Effect of Trench Width and Depth on Film Cooling from Cylindrical Holes Embedded in Trenches 显示文摘	Lu Y P Bunker R S Dhungel A	2009	Journal of Trrbomachinery2009，131，1：	1
	3	Novel low-cost approach for removal of surface contamination before texturization of commercial monoerystalline silicon solar cells 显示文摘	GANGOPADHYAY U DHUNGEL S K MONDAL A K	2007	Solar Energy Materials & Solar Cells2007，91，：	1
	4	Film cooling from a row of holes supplemented with antivortex holes 显示文摘	Dhungel A Lu Yiping Phillips W	2009	Journal of Turbo Machinery2009，131，4：	1
	5	Novel low-cost approach for removal of surface contamination before texturization of commercial monocrystalline silicon solar cells显示文摘	Gangopadhyay U Dhungel S K Mondal A K	2007	Solar Energy Materials and Solar Cells2007，91，12：	1
	6	Novel low-cost approach for removal of surface contamination before texturization of commercial mono crystalline silicon solar cells 显示文摘	GANGOPADHYAY U DHUNGEL S K MONDAL A K	2007	Solar Energy Materials and Solar Cells2007，91，12：	1
	7	Novel low-cost approach for removal of surface contamination before texturization of commercial monocrystalline silicon solar cells显示文摘	GANGOPADHYAY U DHUNGEL S K MONDAL A K	2007	Solar Energy Materials and Solar Cells2007，91，12：	1
	8	Film cooling from a row of holes supplemented with antivortex holes显示文摘	Dhungel A Yu L Philips W	2009	Journal of Turbomachinery2009，131，2：	1
	9	Film cooling from a row of holes supplemented with antivortex holes显示文摘	Dhungel A Lu Y Wynn P V	2009	Jour- nal of Turbomachinery2009，，02：	1
	10	Effect of trench width and depth on film cooling from cylindrical holes embedded in trenches 显示文摘	Lu Yiping Dhungel A Ekkad S V	2009	Journal of Turbomachinery2009，131，01：	1
	11	Effect of trench width and depth on film cooling from cylindrical holes embeded in trenches显示文摘	Lu Y P Dhungel A Ekkad S V	2009	Journal of Turbomachinery2009，131，1：	1
	12	Novel low-cost approach for removal of surface contamination before texturization of commercial monocrystalline silicon solar cells显示文摘	Gangopadhyay U Dhungel S K Mondal A K	2007	Solar Energy Materials and Solar Cells2007，91，12：	1

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