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| 1 | Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma显示文摘Using suppression subtractive hybridization, a renal cell carcinoma (RCC) cDNA subtractive library which only contains differently expressed cDNAs between human RCC and normal kidney has been constructed. 200 clones were picked out randomly to perform enzyme digest analysis, a part of them underwent sequence analysis and Northern blot to identify RCC specially expressed genes. Results showed that 190 clones contain 50-400 bp inserts respectively. Sequence analysis was performed in 10 clones. All the 10 sequences were unknown before and derived from 6 unique novel genes among which the cDNA insert RCC18 has five copies. Northern blot analysis showed that RCC18 cDNA expressed highly in RCC, but there was no signal detected in normal kidney, and the full length of RCC18 was about 2.5 kb. The constructed cDNA subtractive library of human RCC is a highly efficient one and lays the solid foundation for large-scale screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel | ZHANG Qiang ZHANG Zhiwen GONG Kan XIN Dianqi NA Yanqun GUO Yinlu | 2001 | Chinese Science Bulletin2001，46，3： | 8 |
| 2 | Interaction of hTCF4 by yeast two-hybrid system显示文摘To investigate the interaction of hTCF4, the yeast two-hybrid system has been used for testing the interaction of mutants of hTCF4 with themselves. Mutants of hTCF4 (hTCF4 Ⅰ ) and hTCF4 Ⅱ) have been obtained by polymerase chain reaction (PCR). Bait (hTCF4 Ⅰ -pDBLeu and hTCF4Ⅱ-pDBLeu) and prey (hTCF4 Ⅰ -pPC86 and hTCF4Ⅱ-pPC86) have been constructed by DNA recombination for yeast two-hybrid. Interaction of hTCF4Ⅱ with itself is found in the reverse yeast two-hybrid system (GIBCOBRL Co.). However, no interactions are found in hTCF4Ⅱ with hTCF4 Ⅰ and in hTCF4 Ⅰ with hTCF4 Ⅰ . These results suggest that hTCF4 could interact with itself to form homodimer or homocopolymer and perform the transcriptional activating function through LZ or HLH motifs in nucleus of renal cell carcinoma. | LIN Guiting ZHANG Zhiwen ZANG long XIN Dianqi CHANG Zhijie GUO Yinglu | 2000 | Chinese Science Bulletin2000，45，21： | 1 |
| 3 | Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1显示文摘 | Pengxiu Cao Jian Song Chunjiang Zhou Manli Weng Jing Liu Fuxin Wang Feng Zhao Dianqi Feng Bin Wang | 2009 | Plant Molecular Biology2009，，5： | 1 |
| 4 | Aberrant activity of Wnt/Frizzled signaling pathway in renal cancer cell lines显示文摘The expression of Wnt, Wnt receptor-Frizzled, and several other key components in Wnt pathway in renal cancer cell lines was studied. The result of semi-quantitative RT-PCR has shown that the expression level of Wnt5A and hFz5 mRNA were higher in renal cancer cell line (GRC-1) than in normal renal cell line (HK-2). This result has been confirmed by in situ hybridization. The expression of p-catenin protein was obviously higher in GRC-1 than in HK-2 (P<0.01), but there were no different expressions of its mRNA between 3 lines. The reasons of the overexpression of β-catenin has been investigated by means of immunocytochemistry, SSCP and so on, no mutation of β-catenin gene and APC were found. That means that the overexpression of Wnt5A/hFz5 might be the reason of overexpression of β-catenin. It was concluded that the aberrant activity of Wnt pathway might play an important role in renal cell carcinoma. | ZANG Tong ZHUANG Liyan ZHANG Zhiwen XIN Dianqi GUO Yinglu | 2000 | Chinese Science Bulletin2000，45，18： | 1 |
| 5 | PKB negatively modulates TGF-β responsiveness in prostate carcinoma PC-3 cells through its interaction with Smad3显示文摘Most prostate cancers are insensitive to growth-inhibitory effect of TGF-β, while PI3K-PKB signaling is highly activated in prostate cancers. We investigated whether the PI3K-PKB signaling con- tributes to TGF-β insensitivity in PTEN-null prostate cancer PC-3 cells. Cell growth analysis showed that inhibition of PI3K-PKB pathway by LY294002 en- hanced growth inhibition and cell cycle arrest induced by TGF-β. Furthermore, activation of PI3K-PKB pathway by insulin or overexpression of PKB de- creased the transcriptional activity of TGF-β, as measured by the TGF-β/Smad3-responsive CAGA- luciferase reporter, while inhibition of PI3K-PKB pathway by introducing PTEN, inactive PKB mutant or using LY294002 promoted TGF-β-induced expres- sion of CAGA-luciferase. Co-immunoprecipitation studies further demonstrated that Smad3 interacted with PKB through its linker region and MH2 domain. This interaction was facilitated by insulin and disrupted by TGF-β signaling activation. Our results suggest that the PI3K-PKB pathway may play an important role in rendering cell resistance to the antiproliferative effect of TGF-β and regulating cell response to TGF-β. | LI Wei, XIN Dianqi & GUO Yinlu Department of Urology, the First Hospital of Peking University the Urological Research Institute, Peking University, Beijing 100034, China | 2006 | Chinese Science Bulletin2006，51，13： | 1 |
| 6 | A poly functional pendulum apparatus of Ke-type显示文摘 | Dianqi Geng Hairong Wei | 1989 | ICIFUAS-91989，，： | 1 |
| 7 | RNA-seq analysis reveals the critical role of the novel lnc RNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway显示文摘Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including adipogenesis.Research on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in livestock,such as the content of intramuscular fat(IMF).However,the significance of lncRNAs in intramuscular adipogenesis remains unclear.This research aimed to reveal the lncRNAs transcriptomic profiles in the process of bovine intramuscular adipogenesis and to identify the lncRNAs involved in the adipogenesis of bovine intramuscular adipocytes.Results In this research,a landscape of lncRNAs was identified with RNA-seq in bovine intramuscular adipocytes at four adipogenesis stages(0 d,3 d,6 d,and 9 d after differentiation).A total of 7035 lncRNAs were detected,including 3396 novel lncRNAs.Based on the results of differential analysis,co-expression analysis,and functional prediction,we focused on the bovine intramuscular adipogenesis-associated long non-coding RNA(BIANCR),a novel lncRNA that may have an important regulatory function.The knockdown of BIANCR inhibited proliferation and promoted apoptosis of intramuscular preadipocytes.Moreover,BIANCR knockdown inhibited intramuscular adipogenesis by regulating the ERK1/2 signaling pathway.Conclusion This study obtained the landscape of lncRNAs during adipogenesis in bovine intramuscular adipocytes.BIANCR plays a crucial role in adipogenesis through the ERK1/2 signaling pathway.The results are noteworthy for improving beef meat quality,molecular breeding,and metabolic disease research. | Xinhao Ma Xinran Yang Dianqi Zhang Wenzhen Zhang Xiaoyu Wang Kuncheng Xie Jie He Chugang Mei Linsen Zan | 2023 | Journal of Animal Science and Biotechnology2023，14，3： | 1 |
| 8 | Research on method of pressure grouting piling of driven tube显示文摘The pressure grouting pile of driven tube can improve the load bearing capacity of the single pile from the mechanism of pressure grouting pile of driven tube.On the basis of analyzing the mechanism,the authors designed the machines and tools of pressure grouting,determined the operating manufacture and technology parameter on the pressure grouting secondly.The result shows that the pressure grouting pile of driven tube not only changes the pile type but also reduce the length of the pile and its engineering cost,it enhances the load bearing capacity of single pile an the same time. | Dianqi PAN Zupei ZHANG Diancai PAN Yong CHEN Maosen TAN | 2006 | Global Geology2006，9，2： | 0 |
| 9 | Fully Conjugated Covalent Organic Frameworks:Synthesis,Structures and Applications显示文摘This review presents a comprehensive examination of fully conjugated covalent organic frameworks(COFs),which constitute an emerging class of porous materials with immense potential for diverse applications.This article focuses on diversified fully conjugated COFs,including sp2 carbon-carbon linkages,pyrazine linkages,benzobisoxazole linkages,dioxin linkages,β-aminoalkenone linkages,etc.The synthesis techniques and structural attributes of these COFs are expounded upon in great detail,along with their potential applications in various fields.The review thus provides a valuable resource for researchers keen on delving into the synthesis and applications of fully conjugated COFs,thereby highlighting their potential for developing novel functional materials with distinctive properties. | Yueshuai Xu Guanshi Ren Dianqi Zhang Lishui Sun Yingjie Zhao | 2023 | Chinese Journal of Chemistry2023，41，23： | 0 |
| 10 | Challenges and Responses of Left-Behind Elderly and Children in Rural China Amid the New Population Development Stage显示文摘The disparate economic growth in China has compelled numerous adults from rural areas to migrate to cities in search of more lucrative employment prospects(1).In the 7th National Census,it was reported that the floating population totaled 375,816,759,accounting for approximately 26.00%of the overall population.Such migratory patterns often result in leaving behind vulnerable groups,particularly children and older adults,in rural regions.Given the inadequately established care systems,these groups are particularly susceptible and have consistently been at the forefront of social security concerns in China(2). | Dianqi Yuan Peisen Yang Hanbing Yang Huameng Tang Chao Guo | 2023 | China CDC weekly2023，5，27： | 0 |
| 11 | INTERNAL FRICTION OF GRAIN BOUNDARIES IN Fe-P AND Fe-P-La ALLOYS CONTAINING TRACE Cu显示文摘The grain boundary internal friction(GBIF)in Fe-P and Fe-P-La alloys containing traceCu has been studied.It is found that an addition of 0.584—0.722 wt-% La obviously reducesthe IF,shifts grain boundary peaks towards higher temperatures,and produces a small addi-tional peak at even higher temperature.The mechanism of GBIF is explained as the movementof defects of atomic size,namely point defects,at gram boundaries and/or their clusters un-der stress. | JI Jingwen WEI Quanjin GENG Dianqi WU Yuqin Northeast University of Technology,Shenyang,China Professor,Dept.of Physics,Northeast University of Technology,Shenyang 110006,China | 1990 | Acta Metallurgica Sinica(English Letters)1990，3，4： | 0 |
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