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| 1 | Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model显示文摘AIM: To evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns.METHODS: We treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence)and the tight-junction protein occludin and zonula occluden-1.RESULTS: The treated spheroids had deep holes and surface blebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin filaments, impaired and disassembled the integrity of the tight-junction system.CONCLUSION: Our data obtained from an 'in vivolike' polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions. | Ersilia Dolfini Leda Roncoroni Luca Elli Chiara Fumagalli Roberto Colombo Simona Ramponi Fabio Forlani Maria Teresa Bardella | 2005 | World Journal of Gastroenterology2005，11，48： | 3 |
| 2 | Characterization of a clonal human colon adenocarcinom a line intrinsically resistant to doxorubicin显示文摘 | Dolfini E Dasdia T Arancia G | 1997 | Br J Cancer1997，76，1： | 1 |
| 3 | NF-Y recruits Ash2L to impart H3K4 trimethylation on CCAAT promoters显示文摘 | Fossati A Dolfini D Donati G | 2011 | Plos One2011，6，17： | 1 |
| 4 | ClinicalPharmacokinetics of Lercanidipine 显示文摘 | Barchielli M Dolfini E Farina P | 1997 | J CardiovascPharmacol1997，29，2： | 1 |
| 5 | Characterization of a Clonal Human Colon Adenocarcinoma Line Intrinsically Resistance to Doxorrubicin显示文摘 | Dolfini E Dasdia T Arancal G | 1997 | Br J Cancer1997，76，1： | 1 |
| 6 | Resveratrol inhibits cell growth in a human cholangiocarcinoma cell line显示文摘 | Roncoroni L Elli L Dolfini E | 2008 | Liver International:Official Journal of the International Association for the Study of the Liver2008，28，10： | 1 |
| 7 | Resvera- trol impairs the formation of MDA-MB-231 multicellular tumor spheroids concomitant with ceramide accumulation显示文摘 | DOLFINI E RONCORONI L DOGLIOTTI E | 2007 | Cancer Letters2007，249，2： | 1 |
| 8 | Studies on low-level MDR ceils显示文摘 | Belvedere G Dolfini E | 1993 | Cytotechnology1993，12，2： | 1 |
| 9 | Clinical phar- macokinefics of lercanidipine 显示文摘 | BARCHIELLI M DOLFINI E FARINA P | 1997 | J Cardiovasc Pharmacol1997，29，2： | 1 |
| 10 | Characterzation of a clonal human colon adenocarcinoma line intrinsically resistant to doxorubicin显示文摘 | Dolfini E Dasdia T Arancia G | 1997 | Br J Cancer1997，76，1： | 1 |
| 11 | NF-Y and the transcriptional ac- tivation of CCAAT promoters 显示文摘 | Dolfini D Gatta R Mantovani R | 2012 | Crit Rev Bioehem Mol2012，47，1： | 1 |
| 12 | Genetic and molecular analysis of Sn, a light-induccible, tissue specific regulatory gene in maize显示文摘 | Consonnic G Dolfini S F | 1991 | Mol Gen Genet1991，225，： | 1 |
| 13 | Role of the lung resistance-related protein(LRP) in the drug sensitivity of cultured tumor cells显示文摘 | Meschini S Macra M Calcabrini A Monti E Gariboldi M Dolfini E | 2002 | Vitro2002，16，4： | 1 |
| 14 | Characterization of a clonal human colon adenocarcinoma line intrinsically resistant to doxorubicin 显示文摘 | Dolfini E Dasdia T Arancia G | 1997 | Br J Cancer1997，76，1： | 1 |
| 15 | YB-1(YBX1)does not bind to Y/CCAAT boxes invivo显示文摘 | Dolfini D Mantovani R | 2013 | Oncogene2013，32，5： | 1 |
| 16 | NF-Y and the transcriptional activation of CCAAT promoters显示文摘 | Diletta Dolfini Raffaella Gatta Roberto Mantovani | 2012 | Critical Reviews in Biochemistry and Molecular Biology2012，，1： | 1 |
| 17 | Studies on low-level MDR cells显示文摘 | Belvedere G Dolfini E | | 0，，： | 1 |
| 18 | Damaging effects of gliadin on three-dimensional cell culture model显示文摘AIM: To evaluate the effects of gliadin on the oxidative environment in the'in vivo-like' model of a three-dimensional cell culture system.METHODS: LoVo cell line (intestinal adenocarcinoma)multicellular spheroids were treated with digested gliadin (with albumin used as a control). Spheroid volumes, cell viability and morphology, lactate dehydrogenase (LDH)release, content of reduced glutathione (GSH) and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student's t-test (P<0.05).was considered statistically significant.RESULTS: Gliadin reduced cell viability (from 20% to 60%)and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced (-20% vs controls),and activity of GSH-related enzymes was significantly inhibited.CONCLUSION: Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain the cell damage directly caused by gliadin and the subsequent morphological abnormalities. | Ersilia Dolfini Luca Elli Leda Roncoroni Barbara Costa Maria Pia Colleoni Vito Lorusso Simona Ramponi Paola Braidotti Stefano Ferrero Maria Letizia Falini Maria Teresa Bardella | 2005 | World Journal of Gastroenterology2005，11，38： | 0 |
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