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| 1 | In vitro resistance to interferon of hepatitis B virus with precore mutation显示文摘AIM: Chronic hepatitis B virus (HBV) infection is predominantly treated with interferon alpha (IFN-α), which results in an efficient reduction of the viral load only in 20-40% of treated patients. Mutations at HBV precore prevail in different clinical status of HBV infection. The roles of precore mutation in the progression of chronic hepatitis and interferon sensitivity are still unknown. The aim of this study was to explore if there was any relationship between HBV precore mutation and sensitivity to interferon in vitro.METHODS: HBV replication-competent recombinant constructs with different patterns of precore mutations were developed. Then the recombinants were transiently transfected into hepatoma cell line (Huh7) by calcium phosphate transfection method. With or without IFN, viral products in culture medium were collected and quantified 3d after transfection.RESULTS: We obtained 4 recombinant constructs by orientation-cloning 1.2-fold-overlength HBV genome into pUC18 vector via the EcoRI and Hind Ⅲ and PCR mediated site-directed mutagenesis method. All the recombinants contained mutations within precore region. Huh7 cells transfected with recombinants secreted HBsAg and HBV particles into the cell culture medium, indicating that all the recombinants were replication-competent. By comparing the amount of HBV DNA in the medium, we found that HBV DNA in medium reflecting HBV replication efficiency was different in different recombinants. Recombinants containing precore mutation had fewer HBV DNAs in culture medium than wild type. This result showed that recombinants containing precore mutation had lower replication efficiency than wild type. HBV DNA was decreased in pUC18-HBV1.2-WT recombinants after IFN was added while others with precore mutations were not, indicating that HBV harboring precore mutation was less sensitive to IFN in cell culture system.CONCLUSION: These data indicate that HBV harboring precore mutation may be resistant to IFN in vitro. | YanWang LaiWei DongJiang XuCong RanFei JiangXiao YuWang | 2005 | World Journal of Gastroenterology2005，11，5： | 10 |
| 2 | Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses显示文摘AIM: To determine whether dendritic cells (DCs) from chronic hepatitis B patients could induce HBV antigenspecific T cell responses or not. METHODS: DCs were generated from peripheral blood mononuclear cells of patients with chronic hepatitis B (CHB) infection and healthy donors. We compared the phenotypes of these DCs and their ability to secrete cytokines and to participate in mixed lymphocyte reactions. In addition, autologous lymphocytes were cultured with DCs loaded with HBV core region peptide HBcAg8-27, an epitope recognized by cytotoxic T lymphocytes(CTL), and bearing human leucocyte antigen (HLA)-A2 for 10 d. Cytokine secretion and lytic activity against peptide-pulsed target cells were assessed. RESULTS: DCs with typical morphology were generated successfully by culturing peripheral blood mononuclear cells (PBMCs) from CriB patients with AIM-V containing GM-CSF and IL-4. Compared with DCs from normal donors, the level of CD80 expressed in DCs from CHB patients was lower, and DCs from patients had lower capacity of stimulate T cell proliferation. When PBMCs isolated from patients with chronic or acute hepatitis B infection and from normal donors were cocultured with HBcAg18-27 peptide, the antigen-specific memory response of PBMCs from acute hepatitis B patients was stronger than that of PBMCs from chronic hepatitis B patients or normal donors. PBMCs cocultured with DCs treated with HBcAg18-27 CTL epitope peptide induced an antigen-specific T cell reaction, in which the level of secreted cytokines and lyric activity were higher than those produced by memory T cells. CONCLUSION: DCs from patients with CHB can induce HBV antigen-specific T cell reactions, including secretion of cytokines essential for HBV clearance and for killing cells infected with HBV. | Ruo-BingLi Hong-SongChen YaoXie RanFei XuCong DongJiang Song-XiaWang Laiwei YuWang | 2004 | World Journal of Gastroenterology2004，10，11： | 8 |
| 3 | Additive Manufacturing of Ceramic Structures by Laser Engineered Net Shaping显示文摘Ceramic is an important material with outstanding physical properties whereas impurities and porosities generated by traditional manufacturing methods limits its further industrial applications. In order to solve this problem, direct fabrication of Al2O3 ceramic structures is conducted by laser engineered net shaping system and pure ceramic powders. Grain refinement strengthening method by doping Zr O2 and dispersion strengthening method by doping Si C are proposed to suppress cracks in fabricating Al2O3 structure. Phase compositions, microstructures as well as mechanical properties of fabricated specimens are then analyzed. The results show that the proposed two methods are effective in suppressing cracks and structures of single-bead wall, arc and cylinder ring are successfully deposited. Stable phase of α-Al2O3 and t-Zr O2 are obtained in the fabricated specimens. Micro-hardness higher than 1700 HV are also achieved for both Al2O3 and Al2O3/Zr O2, which are resulted from fine directional crystals generated by the melting-solidification process. Results presented indicate that additive manufacturing is a very attractive technique for the production of high-performance ceramic structures in a single step. | NIU Fangyong WU Dongjiang MA Guangyi ZHANG Bi | 2015 | Chinese Journal of Mechanical Engineering2015，28，6： | 7 |
| 4 | NiFe-based nanostructures on nickel foam as highly efficiently electrocatalysts for oxygen and hydrogen evolution reactions显示文摘Water splitting,as an advanced energy conversion technology,consists of two half reactions,including oxygen evolution reaction(OER)and hydrogen evolution reaction(HER).However,the ideal electrocatalysts are noble metal based catalysts.Their high cost and scarcity in earth seriously restrict the large deployments.Ni Fe-based materials have attracted great attention in recent years due to their excellent catalytic properties for OER and HER.Nevertheless,their conductivity and electrochemical stability at high current density are unsatisfactory,resulting in ineffective water splitting due to high impedance and low stability.Recently,a series of catalysts coating Ni Fe-based materials on 3 D nickel foam were found to be extremely stable under the circumstance of high current density.In this review,we summarized the recent advances of NiFe-based materials on nickel foam for OER and HER,respectively,and further provided the perspectives for their future development. | Wei Zhang Daohao Li Longzhou Zhang Xilin She Dongjiang Yang | 2019 | Journal of Energy Chemistry2019，28，12： | 6 |
| 5 | Enhanced photodynamic therapy of mixed phase TiO2(B)/anatase nanofibers for killing of HeLa cells显示文摘光力学的治疗(太平洋夏季时间) 是使用使药具有感光性有选择地使用治疗指向地点的一个过程,被证明了是为可以发展成癌症的癌症和条件的安全治疗。缩放 Nano 的 TiO < 潜水艇 class= “ a-plus-plus ” > 2 为紫外轻驾驶太平洋夏季时间被认为是潜在的 photosensitizer。在这研究, TiO 的四种类型<潜水艇class=“ a-plus-plus ”> 2 nanofibers 从质子 tri-titanate 被准备( H <潜水艇class=“ a-plus-plus ”> 2 T <潜水艇class=“ a-plus-plus ”> 3 O <潜水艇class=“ a-plus-plus ”> 7 ) nanofiber 。同样获得的 nanofibers 为太平洋夏季时间杀死 HeLa 房间作为有效 photosensitizers 被表明。MTT 试金和流动 cytometry (FCM ) 被执行评估 biocompatibility, apoptotic 房间的百分比,和房间生存能力。同样准备的 TiO 的 non-cytotoxicity < 潜水艇 class= “ a-plus-plus ” > 当紫外照耀不在时的 2 nanofibers 也被表明了。在紫外轻照耀下面, TiO < 潜水艇 class= “ a-plus-plus ” > 2 nanofibers,特别地混合阶段 nanofibers,比 Pirarubicin (THP ) 显示了高得多的杀死房间的效率,它是普通的药导致 HeLa 房间的 apoptosis。我们归功于混合阶段 nanofibers 的高 cellkilling 效率到在 TiO 之间的 bandgap 边比赛和稳定的接口 < 候补选手 class= “ a-plus-plus ” > 2 (B) 和锐钛矿在单个 nanofiber 分阶段执行,它能禁止 photogenerated 电子和洞的再结合。这支持费用分离和转移过程和罐头生产负责杀死 HeLa 房间的更多的反应的氧种类(ROS ) 。 | Shuchao Zhang Dongjiang Yang Dengwei Jing Hongwei Liu Long Liu Yi Jia Meihua Gao Liejin Guo Ziyang Huo | 2014 | Nano Research2014，7，11： | 6 |
| 6 | Analysis of the mass of behind-armor debris generated by RHA subjected to normal penetration of variable cross-section EFP显示文摘Analyzing the mass of behind-armor debris (BAD) generated by Rolled Homogeneous Armor (RHA) subjected to normal penetration of variable cross-section Explosively Formed Projectile (EFP) is the purpose of this paper. So theoretical analysis, numerical simulation and experimental data are combined to analyze the influence of variable cross-section characteristic on the time history of crater radius. Moreover the relationships between time history of crater radius (as well as mass of BAD) and the thickness of RHA (from 30mm to 70 mm) and the impact velocity of EFP (1650 m/s to 1860 m/s) are also investigated. The results indicate that: 1) being compared to the variable cross-section characteristic is ignored, the theoretical time history of crater radius is in better agreement with the simulation results when the variable cross-section characteristic is considered;2) being compared to the other three conditions of plug, the theoretical mass of BAD is in the best agreement with the simulation results when the shape of plug is frustum of a cone and the angle between generatrix and bottom is 45- and the axial length of mushroom is considered. | Boyang Xing Dongjiang Zhang Zhenyan Guo Yunhui Hou Rui Guo Rongzhong Liu Liang Chen Hao Zhou Yongliang Yang Jianhua Luo | 2019 | Defence Technology(防务技术)2019，15，3： | 4 |
| 7 | Research progress of nanocellulose for electrochemical energy storage:A review显示文摘Recently, in response to the major challenges in energy development and environmental issues, tremendous efforts are being devoted to developing electrochemical energy storage devices based on green sustainable resources. As a class of green materials, nanocellulose(NC) has received extensive attention. In this review, we summarize the research progress of NC derived materials in electrochemical energy storage. Specifically, we first introduce various synthesis methods based on NC and the pretreatment process to increase the conductivity. Then we focus on the specific application of NC in electrochemical energy storage devices. Finally, we summarize the previously reported work and put forward views on the further development of NC in the field of electrochemical energy storage. | Ruiqi Guo Lixue Zhang Yun Lu Xiaoli Zhang Dongjiang Yang | 2020 | Journal of Energy Chemistry2020，29，12： | 4 |
| 8 | Subsurface Damage in Scratch Testing of Potassium Dihydrogen Phosphate Crystal显示文摘Potassium dihydrogen phosphate(KDP) is an important electro-optic crystal, often used for frequency conversion and Pockels cells in large aperture laser systems.To investigate the influence of anisotropy to the depth of subsurface damage and the profiles of cracks in subsurface of KDP crystal, an experimental study was made to obtain the form of subsurface damage produced by scratches on KDP crystal in 100, 120 and 110 crystal directions on(001) crystal plane.The results indicated that there were great differences between depth and crack shape in different directions.For many slip planes in KDP, the plastic deformation and cracks generated under pressure in the subsurface were complex.Fluctuations of subsurface damage depth at transition point were attributed to the deformation of the surface which consumed more energy when the surface deformation changed from the mixed region of brittle and plastic to the complete brittle region along the scratch.Also, the process of subsurface damage from shallow to deep, from dislocation to big crack in KDP crystal with the increase of radial force and etch pit on different crystal plane were obtained.Because crystallographic orientation and processing orientation was different, etching pits on(100) crystal plane were quadrilateral while on(110) plane and(120) plane were trapezoidal and triangular, respectively. | WANG Ben WU Dongjiang GAO Hang KANG Renke GUO Dongming | 2009 | Chinese Journal of Mechanical Engineering2009，22，1： | 3 |
| 9 | Oxygen Electroreduction on CoSe2 Nanoparticles Prepared via Hydrothermal Method in Acidic Medium显示文摘 | ZHAO Dongjiang MA Songyan | 2015 | Chemical Research in Chinese Universities2015，31，3： | 3 |
| 10 | Hierarchical red phosphorus incorporated TiO_(2) hollow sphere heterojunctions toward superior photocatalytic hydrogen production显示文摘Solar-to-hydrogen conversion through photocatalysis is a sustainable and promising strategy for storing solar energy.Recently,elemental red phosphorus(RP)with broad light absorption has been recognized as a potential candidate for photocatalytic hydrogen evolution,while challenges remain due to the rapid recombination of photogenerated carriers.In this work,RP modified TiO_(2)hollow spheres were designed and fabricated through the chemical vapor deposition method.The optimal hydrogen production rate reaching 215.5μmol/(g h)over TiO_(2)@RP heterostructure was obtained under simulated solar light irradiation.Experimental results evidenced that the hollow sphere structure and RP light absorber extended light absorption ability,and the heterostructure induced interfacial charge migration facilitated photoinduced charge separation,which benefited the photocatalytic hydrogen production performance. | Guiqing Huang Wanneng Ye Chunxiao Lv Denys S.Butenko Chen Yang Gaolian Zhang Ping Lu Yan Xu Shuchao Zhang Hongwei Wang Yukun Zhu Dongjiang Yang | 2022 | Journal of Materials Science & Technology2022，，13： | 3 |
| 11 | Genetic expression in progeny of transgenic plants obtained by using pollen-tube pathway (or delivery) method and approach to the transformation mechanism显示文摘The origin and the recent progress of pollen_tube pathway method (PTPM) is described. The results of transforming wheat during 1990 to 1994 are reported briefly. They are: (ⅰ) Transformation frequencies were 3%-6%, even reached 13.5%; (ⅱ) the genetic expression of four continuous generations obtained from single individuals of transgenic plants by self_fertilization was investigated. Plants with higher expression of foreign gene were found in every generation (from T1 to T4), but a phenomenon of gene silence, in which the genotype contained foreign gene but did not express, was also observed. In addition, from the angle of fertilization biology the transformation mechanism of PTPM (or PTDM) was analyzed and discussed. It was considered that the essential principle of this method was just like that of protoplast method. | Junzhi Zeng Youqiang Wu Dongjiang Wang Jian Zhang Zhengrong Ma Zhiyong Zhou | 1998 | Chinese Science Bulletin1998，43，10： | 3 |
| 12 | Damage mechanisms during lapping and mechanical polishing CdZnTe wafers显示文摘CdZnTe wafers were machined by lapping and mechanical polishing processes,and their surface and subsurface damages were investigated.The surface damages are mainly induced by three-body abrasive wear and embedded abrasive wear during lapping process.A new damage type,which is induced by the indentation of embedded abrasives,is found in the subsurface.When a floss pad is used to replace the lapping plate during machining,the surface damage is mainly induced by two-body abrasive and three-body abrasive wear,and the effect of embedded abrasives on the surface is greatly weakened.Moreover,this new damage type nearly disappears on the subsurface. | LI Yan,KANG Renke,GAO Hang,and WU Dongjiang Key Laboratory for Precision and Non-Traditional Machining Technology (Ministry of Education),Dalian University of Technology,Dalian 116024,China | 2010 | Rare Metals2010，29，3： | 2 |
| 13 | How heteroatoms(Ge,N,P) improve the electrocatalytic performance of graphene:theory and experiment显示文摘The oxygen reduction reactions(ORR)play a crucial role in the electrochemical energy storage devices,such as fuel cell,metal-air batteries[1–3].However,their larger scale applications are hindered by the sluggish slow kinetics of the ORR.Up to now,platinum(Pt)-based catalysts are still known as the best | Guojing Chang Jun Ren Xilin She Kewei Wang Sridhar Komarneni Dongjiang Yang | 2018 | Science Bulletin2018，63，3： | 2 |
| 14 | Investigation of melt-growth alumina/aluminum titanate composite ceramics prepared by directed energy deposition显示文摘Al_(2)O_(3)/Al_(6)Ti_(2)O_(13) composite ceramics with low thermal expansion properties are promising for the rapid preparation of large-scale and complex components by directed energy deposition-laser based(DED-LB)technology.However,the wider application of DED-LB technology is limited due to the inadequate understanding of process conditions.The shaping quality,microstructure,and mechanical properties of Al_(2)O_(3)/Al_(6)Ti_(2)O_(13)(6 mol%TiO_(2))composite ceramics were systematically investigated as a function of energy input in an extensive process window.On this basis,the formation mechanism of solidification defects and the evolution process of microstructure were revealed,and the optimized process parameters were determined.Results show that high energy input improves the fluidity of the molten pool and promotes the uniform distribution and full growth of constituent phases,thus,facilitating the elimination of solidification defects,such as pores and strip gaps.In addition,the microstructure size is strongly dependent on the energy input,increasing when the energy input increases.Moreover,the morphology of theα-Al_(2)O_(3) phase gradually transforms from cellular into cellular dendrite with increasing energy input due to changing solidification conditions.Under the comprehensive influence of solidification defects and microstructure size,the fracture toughness and flexural strength of Al_(2)O_(3)/Al_(6)Ti_(2)O_(13) composite ceramics present a parabolic law behavior as the energy input increases.Optimal shaping quality and excellent mechanical properties are achieved at an energy input range of 0.36-0.54 W*min^(2) g^(-1) mm^(-1).Within this process window,the average microhardness,fracture toughness,and flexural strength of Al_(2)O_(3)/Al_(6)Ti_(2)O_(13) composite ceramics are up to 1640 Hv,3.87 MPa m^(1/2),and 227 MPa,respectively.This study provides practical guidance for determining the process parameters of DED-LB of melt growth Al_(2)O_(3)/Al_(6)Ti_(2)O_(13) composite ceramics. | Yunfei Huang Dongjiang Wu Dake Zhao Fangyong Niu Guangyi Ma | 2021 | International Journal of Extreme Manufacturing2021，3，3： | 2 |
| 15 | Coaxial laser cladding of A1203-13 TiO2 powders on Ti-6AI-4V alloy显示文摘 | CHEN YUNXIAO WU DONGJIANG MA GUAN- GYI | 2012 | Surface Coatings Technology2012，，228： | 1 |
| 16 | Directformation of hydroPhobic silica-based micro/meso-Porous hybrids from Polymethylhydrosiloxane and tet-raethoxysilane显示文摘 | Yang Dongjiang Li JunPing Xu Yao | 2006 | MicroP MesoP Mater2006，95，13： | 1 |
| 17 | Ultrafine polycrystalline titania nanofibers for superior sodium storage显示文摘Sodium ion batteries have a huge potential for large-scale energy storage for the low cost and abundance of sodium resources. In this work, a novel structure of ultrafine polycrystalline TiO2 nanofibers is prepared on nickel foam/carbon cloth by a simple vapor deposition method. The as-prepared TiO2 nanofibers show excellent performance when used as anodes for sodium-ion batteries. Specifically, the TiO2 nanofibers@nickel foam electrode delivers a high reversible capacity of 263.2 m Ahg^-1 at 0.2 C and maintains a considerable capacity of 144.2 m Ahg^-1 at 10 C. The TiO2 nanofibers@carbon cloth electrode also shows excellent high-rate capability, sustaining a capacity of 148 m Ahg^-1 after 20 0 0 cycles at 10 C. It is believed that the novel nanofibrous structure increases the contact area with the electrolyte and greatly shortens the sodium ion diffusion distance, and meanwhile, the polycrystalline nature of nanofibers exposes more intercalation sites for sodium storage. Furthermore, the density functional theory calculations exhibit strong ionic interactions between the exposed TiO2(101) facets and sodium ions, leading to a preferable sodiation/desodiation process. The unique structural features endow the TiO2 nanofibers electrodes great advantages in rapid sodium storage with an outstanding high-rate capability. | Zhidan Diao Daming Zhao Chunxiao Lv Hongli Liu Dongjiang Yang Shaohua Shen | 2019 | Journal of Energy Chemistry2019，28，11： | 1 |
| 18 | Laser bending of brittle materials显示文摘 | Wu Dongjiang Zhang Qiang Ma Guangyi | 2010 | Optics and Lasers in Engineering2010，48，4： | 1 |
| 19 | Laser bending of brittle materials 显示文摘 | Wu Dongjiang Zhang Qiang Ma Guangyi | 2010 | Optics and Lasers in Engineering2010，48，4： | 1 |
| 20 | Polybenzimidazole functionalized electrolyte with Li-wetting and self-fluorination functionalities for practical Li metal batteries显示文摘Rough Li plating,low ionic conductivity,and low thermal stability of conventional electrolytes post-primary challenges for achieving reliable high-capacity rechargeable lithium batteries,for which lithiummetal is frequently proposed as themost promising anode material.Conventional low-polarity commercial polypropylene/polyethylene separators fail to support the application of high-energy-density Li anodes due to their rigid physicochemical properties and the high reactivity of Li metal,leading to fatal dendrite formation and vigorous exothermic reaction with electrolytes.Herein,we develop a Li-wetting,flame-retardant binary polymer electrolyte by functionalizing poly(vinylidene fluoride)(PVDF)separators with nonflammable polybenzimidazole(PBI)to build safe room-temperature solid-state electrolyte membranes.A dendrite-free LiFePO4 cell with the solid polymer electrolyte(SPE)delivers a discharge capacity of 127 mAh g^(-1) at 25℃ with a capacity retention of 87.5%after 500 cycles at 0.5℃(0.15 mA cm^(-2)).Phase-field simulations and density functional theory calculations demonstrate that the negatively charged benzimidazole chains of PBI own superior affinity to lithium bis(trifluoromethanesulfonyl)imide(LiTFSI),and shares overlapping electron density with Li anode,giving rise to accelerated Li^(+)conduction at room temperature and uniform Li electrodeposition at the electrolyte/Li metal interface.The SPE is also flame-retardant since heat-resistant polytetrafluoroethylene and a dense,heat-blocking graphitized carbon layer are formed in intense heat throughdehydrogenation/fluorination of PVDF under the catalysis of Lewis base imidazole rings and the decomposition of benzimidazole rings in PBI.No such fire-resistant mechanism is ever reported in conventional electrolytes. | Dongjiang Chen Yuanpeng Liu Chuan Xia Yupei Han Qingwei Sun Xuchang Wang Wei Chen Xian Jian Weiqiang Lv Jianyi Ma Weidong He | 2022 | InfoMat2022，4，5： | 1 |
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