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| 1 | Drosophila RecQ5 is required for efficient SSA repair and suppression of LOH in vivo显示文摘RecQ5 in mammalian cells has been suggested to suppress inappropriate homologous recombination.However,the specific pathway(s)in which it is involved and the underlining mechanism(s)remain poorly understood.We took advantage of genetic tools in Drosophila to investigate how Drosophila RecQ5(dRecQ5)functions in vivo in homologous recombination-mediated double strand break(DSB)repair.We generated null alleles of dRecQ5 using the targeted recombination technique.The mutant animals are homozygous viable,but with growth retardation during development.The mutants are sensitive to both exogenous DSB-inducing treatment,such as gamma-irradiation,and endogenously induced double strand breaks(DSBs)by I-Sce I endonuclease.In the absence of dRecQ5,single strand annealing(SSA)-mediated DSB repair is compromised with compensatory increases in either inter-homologous gene conversion,or non-homologous end joining(NHEJ)when inter-chromosomal homologous sequence is unavailable.Loss of function of dRecQ5 also leads to genome instability in loss of heterozygosity(LOH)assays.Together,our data demonstrate that dRecQ5 functions in SSA-mediated DSB repair to achieve its full efficiency and in suppression of LOH in Drosophila. | Yixu Chen Wen Dui Zhongsheng Yu Changqing Li Jun Ma Renjie Jiao | 2010 | Protein & Cell2010，1，5： | 5 |
| 2 | Drosophila sbo regulates lifespan through its function in the synthesis of coenzyme Q in vivo显示文摘CoQ 是在 mitochondrial 的一个必要电子搬运人优核质和初核质的呼吸的链。它由一个 benzoquinone 头组和一条恐水病的 polyisoprenoid 尾巴组成。涉及 CoQ 生合成的基因(COQ19 ) 在酵母被描绘了。在这研究,我们产生了并且分子地描绘了新奇果蝇基因的变异的等位基因, sbo,它编码被预言在 CoQ 的过程期间与 isoprenoid 链催化 p-hydroxybenzoate 的 prenylation 的蛋白质合成。在酵母的 sbo 的表示救 COQ2 变异的房间的致命性,显示 sbo 是 COQ2 的一个功能的相当或相同的事物。HPLC 结果证明 CoQ9 和 CoQ10 的层次显著地在异质接合的成年人飞的 sbo 被减少。而且,男性和为 sbo 异质接合的女性的吝啬的 lifespans 被 12.5% 和 30.8% 分别地扩大。同型结合的 sbo 动物展出发信号的 insulin/insulin-like 生长因素(IIS ) 的减少的活动小径。一起拿,我们断定 sbo 是为果蝇开发的必要基因,哪个由改变内长的 CoQ 生合成很可能导致 lifespan 的延期的变化。 | Jiyong Liu QinghuaWu Dianlu He Tengyu Ma Li Du Wen Dui Xiaoyan Guo Renjie Jia | 2011 | Journal of Genetics and Genomics2011，38，6： | 4 |
| 3 | A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila显示文摘F 盒子蛋白质是 SCF (SkpA-Cullin 1-F-box ) 的部件 E3 ligase 建筑群,在为 ubiquitination 和降级指向底层蛋白质充当特性决定因素。在人,至少 22 试验性地从 75 F 盒子蛋白质记录了底层,而在果蝇 12 F 盒子蛋白质与已知的底层被描绘了。系统地在果蝇调查 F 盒子蛋白质的基因、分子的功能,我们执行了文学和数据库的调查。我们识别了编码包含至少一个 F 盒子领域的蛋白质的 45 果蝇基因。我们对这些基因收集了公开地可得到的 RNAi 线并且在一幅织物特定的基于 RNAi 的 phenotypic 屏幕使用了他们。这里,我们在场我们从眼睛,翅膀和 notum 的系统的 phenotypic 数据集。这数据集是第一种它的类型并且在果蝇为 F 盒子基因的分子、基因的函数的未来研究代表一个有用资源。我们的结果显示出那,期望在果蝇的 F 盒子基因包括房间增长在进程的一个多样的数组有规章的角色,房间生长,信号 transduction,并且细胞并且动物幸存。 | Wen Dui Wei Lu Jun Ma Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，8： | 3 |
| 4 | High-efficiency electrocatalytic NO reduction to NH_(3) by nanoporous VN显示文摘Electrocatalytic NO reduction reaction to generate NH_(3)under ambient conditions offers an attractive alternative to the energy-extensive Haber-Bosch route;however,the challenge still lies in the development of cost-effective and high-performance electrocatalysts.Herein,nanoporous VN film is first designed as a highly selective and stable electrocatalyst for catalyzing reduction of NO to NH_(3)with a maximal Faradaic efficiency of 85%and a peak yield rate of 1.05×10^(-7)mol·cm^(-2)·s^(-1)(corresponding to 5,140.8mg·h^(-1)·mg_(cat).^(-1))at-0.6 V vs.reversible hydrogen electrode in acid medium.Meanwhile,this catalyst maintains an excellent activity with negligible current density and NH_(3)yield rate decays over 40 h.Moreover,as a proof-of-concept of Zn-NO battery,it delivers a high power density of 2.0 mW·cm^(-2)and a large NH_(3)yield rate of 0.22×10^(-7)mol·cm^(-2)·s^(-1)(corresponding to 1,077.1mg·h^(-1)·mg_(cat).^(-1)),both of which are comparable to the best-reported results.Theoretical analyses confirm that the VN surface favors the activation and hydrogenation of NO by suppressing the hydrogen evolution.This work highlights that the electrochemical NO reduction is an eco-friendly and energy-efficient strategy to produce NH_(3). | Defeng Qi Fang Lv Tianran Wei Mengmeng Jin Ge Meng Shusheng Zhang Qian Liu Wenxian Liu Dui Ma Mohamed SHamdy Jun Luo Xijun Liu | 2022 | Nano Research Energy2022，1，2： | 2 |
| 5 | A Simple Cement Hydration Model Considering the Influences ofWater-to-Cement Ratio and Mineral Composition显示文摘A simple hydration model is used here by taking the composition of the cement and the initial water: cementratio (w/c) into account explicitly. Its conceptual basis is a combination of the Avrami equation and Bentz’s modelbased on simple spatial considerations. In this model, the Avrami equation determines the initial reaction, andBentz’s model describes the following hydration stage. The model favors engineers for it relies on one experimentalparameter and has a reliable approximation in the practice. | Baoyu Ma Guansuo Dui Zhenglin Jia Bo Yang Chunyan Yang Quangui Gao Longhua Qin Ju Ma | 2021 | Computer Modeling in Engineering & Sciences2021，，6： | 1 |
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