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	题名	作者	年代	出处	被引量
1	Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector显示文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction.	Xiao B Jing B Zhang YL Zhou DY Zhang WD	2000	World Journal of Gastroenterology2000，6，1：	11
2	Proteins encoded by genes involved in chromosomal alterations in lymphoma and leukemia: clinical value of their detection by immunocytochemistry 显示文摘	Falini B Mason DY	2002	Blood2002，99，2：	1
3	The American Red Cross Donor Hemovigilance Program, complications of donation显示文摘	Eder A F Dy B A Kennedy J A	2006	Transfusion2006，46，9：	1
4	Diffusion into use of exenatide for glucose control in diabates mellitus: a retrospective cohort study of a new therapy 显示文摘	SEGAL J B DY S M MILLMAN E A	2007	Clin Ther2007，29，8：	1
5	Neuroradiological find- ings and clinical features of fourth-ventrieular meningioma: a study of 10 eases 显示文摘	Zhang BY Yin B Geng DY	2012	Clinical Radiology2012，67，5：	1
6	Molecular characterization of the first Aichi viruses isolated in Europe and in South America显示文摘	Oh DY Silva PA Haurader B	2006	Arch Virol2006，151，6：	1
7	Technical note: improved DNA extraction from ancient bones using silica-based spin columns 显示文摘	YANG DY Eng B Waye JS	1998	Am J Phys Anthropol1998，105，4：	1
8	Dy3+ and Nd3+in- duced genetic mutation of bacillus alpha-amylase 显示文摘	Zhang DY Hu JH Siqin B	2009	Journal of Inorganic Biochemistry2009，103，7：	1
9	Outcomes of pancreaticoduodenectomy for pancreatic malignancy in octogenarians:an American College of Surgeons National Surgical Quality Improvement Program analysis显示文摘	Lee DY Schwartz JA Wexelman B	2014	Am J Surg2014，207，4：	1
10	Chemical constituents of Pteris multfida and their inhibitory effects on growth of rat prostatic epi- thelial cells in vitro 显示文摘	Qin B Zhu DY Jiang SH	2006	Chin J Nat Med2006，4，6：	1
11	Identification and characterization of a novel cancer/testis antigen gene CAGE显示文摘	Cho B Lim Y Lee DY	2002	Biochem Biophys Res Coun2002，5292，3：	1
12	Li storage properties of disordered graphene nanosheets 显示文摘	Pan DY Wang S Zhao B Wu MH Zhang H J Wang Y Jiao Z	2009	Chem Mater2009，21，：	1
13	Gene mapping for primary open angle glaucoma显示文摘	Fan B J Wang DY Lam	2006	Clin Biochem2006，39，3：	1
14	Electrophysiologic effect of enalapril on guinea pig papillary mescles in vitro显示文摘	Liu B Hu DY Yaan G	1997	Acta Pharmacol Sina1997，18，：	1
15	Low dose leflunomide activates PI3K/Akt signalling in erythroleukemia cells and reduces apoptosis induced by anticancer agents 显示文摘	Leger DY Liagre B Beneytout JL	2006	Apoptosis2006，11，10：	1
16	Lamivudine prophylaxis for hepatitis B virus carrier patients with breast cancer during adjuvant chemotherapy显示文摘	Lee HJ Kim DY Keam B	2014	Breast Cancer2014，21，4：	1
17	Gene mapping for primary open angle glaucoma显示文摘	Fan B J Wang DY Lam DS	2006	Clin Biochem2006，39，：	1
18	Recent advances in sepsis research:novel biomarkers and therapeutic targets显示文摘	Ivády B Béres BJ Szab6 D		0，，21：	1
19	The antidepressant effect of ethanol extract of radix puerariae in mice exposed to cerebral isehemia reperfusion显示文摘	Wang DY Xing DM	2004	Pharmacology Biochemistry & Behavior2004，78，2：	1
20	Thermal oxidative degradation behav- iours of flame-retardant copolyesters containing phosphorous linked pendent group/montmorillonite nanocomposites显示文摘	Wang DY Wang Y Z Wang J S Chen D Q Zhou Q Yang B Li W Y	2005	Polym Degrad Stab2005，87，：	1

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