| 2 | Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time显示文摘Human pluripotent stem cell(h PSC)-derived progenies are immature versions of cells,presenting a potential limitation to the accurate modelling of diseases associated with maturity or age.Hence,it is important to characterise how closely cells used in culture resemble their native counterparts.In order to select appropriate time points of retinal pigment epithelium(RPE)cultures that reflect native counterparts,we characterised the transcriptomic profiles of the h PSC-derived RPE cells from 1-and 12-month cultures.We differentiated the human embryonic stem cell line H9 into RPE cells,performed single-cell RNA-sequencing of a total of 16,576 cells to assess themolecular changes of the RPE cells across these two culture time points.Our results indicate the stability of the RPE transcriptomic signature,with no evidence of an epithelial–mesenchymal transition,and with the maturing populations of the RPE observed with time in culture.Assessment of Gene Ontology pathways revealed that as the cultures age,RPE cells upregulate expression of genes involved in metal binding and antioxidant functions.This might reflect an increased ability to handle oxidative stress as cells mature.Comparison with native human RPE data confirms a maturing transcriptional profile of RPE cells in culture.These results suggest that long-term in vitro culture of RPE cells allows the modelling of specific phenotypes observed in native mature tissues.Our work highlights the transcriptional landscape of h PSC-derived RPE cells as they age in culture,which provides a reference for native and patient samples to be benchmarked against. | Grace E.Lidgerwood Anne Senabouth Casey J.A.Smith-Anttila Vikkitharan Gnanasambandapillai Dominik C.Kaczorowski Daniela Amann-Zalcenstein Erica L.Fletcher Shalin H.Naik Alex W.Hewitt Joseph E.Powell Alice Pebay | 2021 | Genomics, Proteomics & Bioinformatics2021，19，2： | 1 |
| 4 | ITIL走过20年显示文摘制定于20世纪80年代末期的ITIL,是英国政府用于证实IT最佳实践的一个项目,随后发展成IT服务管理的架构,并成为国际标准(ISO/IEC20000)。第二版本是第一版本的升级,出版于2000年,主要是关注在改善过程中应该做什么。第三版本的升级,开始于2004年,关注的是服务驱动的生命周期,主要强调ITIL最佳实践的执行支持,以及在改善过程中需要注意的细节。 | James E.Powell | 2007 | 软件世界2007，，15： | 0 |
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