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| 1 | Identification of mandarin hybrids by isozyme and RAPD analysis显示文摘 | ElisiaArio Paulo J Justo Edite M LeitaAo JoseA M | 1999 | Scientia Horticulturae1999，81，： | 1 |
| 2 | Identification of mandarin hybrids by isozyme and RAPD analysis 显示文摘 | Paulo J E Edite M J Jose M L | 1999 | Scientia Horticulturae1999，81，： | 1 |
| 3 | Neural network classification of Remote sensing data显示文摘 | DIANE M EDIT J SORAYA R | 1995 | Computers &Geosciences1995，21，3： | 1 |
| 4 | Successful pregnancy and delivery after calcium ionophore oocyte activation in a normozoospermic patient with previous repeated failed fertilization after intracytoplasmic sperm injection显示文摘 | Talia Eldar-Geva Baruch Brooks Ehud J Margalioth Edit Zylber-Haran Michael Gal Sherman J Silber | 2003 | Fertility and Sterility2003，，： | 1 |
| 5 | Identification of two hsp90 genes in carp显示文摘 | Magdolna A János N | 2001 | Comp Biochem Phys Part C2001，129，7： | 1 |
| 6 | 2 Medication-overuse headache显示文摘 | Silberstein SD Olesen J Bousser MG The International Classification of Headache Disorders 2nd Edition(ICHD-Ⅱ)- revision of criteria for 8 | 2005 | Cephalalgia2005，25，6： | 1 |
| 7 | Immunochemical detection of ochratoxin A in black Aspergillus strains显示文摘 | József Téren János Varga Zsuzsanna Hamari Edit Rinyu Ferenc Kevei | 1996 | Mycopathologia1996，，3： | 1 |
| 8 | Neural network classification of remote sensing data显示文摘 | Diane M Miller Edit J Kaminsky Soraya Rana | 1995 | Computers & Geosciences1995，21，3： | 1 |
| 9 | Real-world performance analysis of a novel computational method in the precision oncology of pediatric tumors显示文摘Background The utility of routine extensive molecular profiling of pediatric tumors is a matter of debate due to the high number of genetic alterations of unknown significance or low evidence and the lack of standardized and personalized decision support methods.Digital drug assignment(DDA)is a novel computational method to prioritize treatment options by aggregating numerous evidence-based associations between multiple drivers,targets,and targeted agents.DDA has been validated to improve personalized treatment decisions based on the outcome data of adult patients treated in the SHIVA01 clinical trial.The aim of this study was to evaluate the utility of DDA in pediatric oncology.Methods Between 2017 and 2020,103 high-risk pediatric cancer patients(<21 years)were involved in our precision oncology program,and samples from 100 patients were eligible for further analysis.Tissue or blood samples were analyzed by whole-exome(WES)or targeted panel sequencing and other molecular diagnostic modalities and processed by a software system using the DDA algorithm for therapeutic decision support.Finally,a molecular tumor board(MTB)evaluated the results to provide therapy recommendations.Results Of the 100 cases with comprehensive molecular diagnostic data,88 yielded WES and 12 panel sequencing results.DDA identified matching off-label targeted treatment options(actionability)in 72/100 cases(72%),while 57/100(57%)showed potential drug resistance.Actionability reached 88%(29/33)by 2020 due to the continuous updates of the evidence database.MTB approved the clinical use of a DDA-top-listed treatment in 56 of 72 actionable cases(78%).The approved therapies had significantly higher aggregated evidence levels(AELs)than dismissed therapies.Filtering of WES results for targeted panels missed important mutations affecting therapy selection.Conclusions DDA is a promising approach to overcome challenges associated with the interpretation of extensive molecular profiling in the routine care of high-risk pediatric cancers.Knowledgebase updates enable automatic interpretation of a continuously expanding gene set,a“virtual”panel,filtered out from genome-wide analysis to always maximize the performance of precision treatment planning. | Barbara Vodicska Júlia Déri Dóra Tihanyi Edit Várkondi EnikőKispéter Róbert Dóczi Dóra Lakatos Anna Dirner Mátyás Vidermann Péter Filotás Réka Szalkai-Dénes István Szegedi Katalin Bartyik Krisztina Míta Gábor Réka Simon Péter Hauser György Péter Csongor Kiss Miklós Garami István Peták | 2023 | World Journal of Pediatrics2023，19，10： | 0 |
| 10 | Diabetes-related intestinal region-specific thickening of ganglionic basement membrane and regionally decreased matrix metalloproteinase 9 expression in myenteric ganglia显示文摘BACKGROUND The importance of the neuronal microenvironment has been recently highlighted in gut region-specific diabetic enteric neuropathy. Regionally distinct thickening of endothelial basement membrane(BM) of intestinal capillaries supplying the myenteric ganglia coincide with neuronal damage in different intestinal segments. Accelerated synthesis of matrix molecules and reduced degradation of matrix components may also contribute to the imbalance of extracellular matrix dynamics resulting in BM thickening. Among the matrix degrading proteinases, matrix metalloproteinase 9(MMP9) and its tissue inhibitor(TIMP1) are essential in regulating extracellular matrix remodelling.AIM To evaluate the intestinal segment-specific effects of diabetes and insulin replacement on ganglionic BM thickness, MMP9 and TIMP1 expression.METHODS Ten weeks after the onset of hyperglycaemia gut segments were taken from the duodenum and ileum of streptozotocin-induced diabetic, insulin-treated diabetic and sex-and age-matched control rats. The thickness of BM surrounding myenteric ganglia was measured by electron microscopic morphometry. Wholemount preparations of myenteric plexus were prepared from the different gut regions for MMP9/TIMP1 double-labelling fluorescent immunohistochemistry. Post-embedding immunogold electron microscopy was applied on ultrathin sections to evaluate the MMP9 and TIMP1 expression in myenteric ganglia and their microenvironment from different gut segments and conditions. The MMP9 and TIMP1 messenger ribonucleic acid(m RNA) level was measured by quantitative polymerase chain reaction.RESULTS Ten weeks after the onset of hyperglycaemia, the ganglionic BM was significantly thickened in the diabetic ileum, while it remained intact in the duodenum. The immediate insulin treatment prevented the diabetes-related thickening of the BM surrounding the ileal myenteric ganglia. Quantification of particle density showed an increasing tendency for MMP9 and a decreasing tendency for TIMP1 from the proximal to the distal small intestine under control conditions. In the diabetic ileum, the number of MMP9-indicating gold particles decreased in myenteric ganglia, endothelial cells of capillaries and intestinal smooth muscle cells, however, it remained unchanged in all duodenal compartments. The MMP9/TIMP1 ratio was also decreased in ileal ganglia only. However, a marked segment-specific induction was revealed in MMP9 and TIMP1 at the m RNA levels.CONCLUSION These findings support that the regional decrease in MMP9 expression in myenteric ganglia and their microenvironment may contribute to extracellular matrix accumulation, resulting in a region-specific thickening of ganglionic BM. | Nikolett Bódi Diána Mezei Payal Chakraborty Zita Szalai Bence Pál Barta János Balázs Zsolt Rázga Edit Hermesz Mária Bagyánszki | 2021 | World Journal of Diabetes2021，12，5： | 0 |
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