|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effects of icariin on cGMP-specific PDE5 and cAMP-specific PDE4 activities显示文摘Aim:To clarify the mechanism of the therapeutic action of icariin on erectlile dysfunction(ED).Methods:PDE5 was isolated from the human platelet and PDE4 form the rat liver tissue using the FPLC system (Pharmacia,Milton Keynes,UK)and the Mono Q column.The inhibitory effects of icariin on PDE5 and PDE4 activities were investigated by the two-step radioisotope procedure with [^3H]-c GMP/[^3H]-cAMP.Papaverine served as the control drug.Results:Icariin and papaverine showed dose-dependent inhibitory effects on PDE5 and PDE4 activities.The IC50 of Icariin and papaverine on PDE5 were 0.432μ mol/L and 0.680μmol/L,respectively and those on PDE4,73.50μmol/L and 3.07μmol/L,respectively.The potencies of selectivity of icariin and papaverine on PDE5(PDE4/PDE5 of IC50)were 167.67 times and 4.54 times,respectively.Conclusion:Icariin is a cGMP-specific PDE5 inhibitor that may be developed into an oral effective agent for the treatment of ED. | ZC Xin EK Kim CS Lin WJ Liu L Tian YM Yuan J Fu | 2003 | Asian Journal of Andrology2003，5，1： | 38 |
| 2 | Significant decrease in prevalence ofHelicobacter pylori in the Czech Republic显示文摘AIM:To study possible decrease in prevalence of Helicobacter pylori(H.pylori) infection in the Czech Republic within a 10-year period.METHODS:A total of 22 centres entered the study.The catchment areas of these centres covered cities and towns with more than 20 000 inhabitants,smaller towns(≤ 20 000 inhabitants) with surrounding villages and rural areas,and were spread over the whole country,corresponding well to the geographical distribution of the Czech population.A total of 1 837 subjects(aged 5-98 years) took part in the study,randomly selected out of 38 147 people from the general population.H.pylori infection was investigated by means of a 13 C-urea breath test.Breath samples in duplicates were analysed using isotope ratio mass spectrometry.The cut-off point was 3.5.Social and demographic characteristics were based on data from self-completed questionnaires.RESULTS:The overall prevalence of H.pylori infection was 23.5%(430/1826),and 4.8%(20/420) in children aged 15 or less.There was no statistically significant difference in prevalence between males(24.3%;208/857) and females(22.9%,222/969,P = 0.494).H.pylori infection was strongly associated with higher age,among subjects aged 55+ years,prevalence of H.pylori infection was 39.8%(252/633,P < 0.001).The highest prevalence of H.pylori infection was found among persons aged 55-64 years(43.9%,97/221) and 75+ years(37.9%,58/153).Among study subjects aged 15+ years,prevalence of H.pylori infection was significantly increased in those with lowest education(odds risk 3.19,95% CI 1.87-5.47).Compared to never married(14.1%),the prevalence of H.pylori infection was statistically significantly higher among married(35.4%,246/694,P < 0.001),divorced(36.8%,49/133,P < 0.001) and widowed study subjects(40.2%,45/112,P < 0.001),both in minimally and fully adjusted analysis.There was no significant difference in the prevalence of H.pylori infection between married and widowed subjects(35.4%,246/694 vs 40.2%,45/112,P = 0.389).There was little variation in smoking prevalence across categories of smoking and there was no evidence of an increased risk ofH.pylori infection among current or past smokers in our data(odds risk 1.04 with 95% CI 0.78-1.40 for current smokers;odds ratio 0.83 with 95% CI 0.60-1.16 for former smokers).The current prevalence of H.pylori in 2011 was significantly lower compared to the prevalence reported from identical geographical areas in 2001(23.5%vs 41.7%,P < 0.001).CONCLUSION:The overall prevalence of H.pylori infection in the general population has fallen substantially in the Czech Republic over the past 10 years. | Jan Bure Marcela Kopáová Ilona Koupil Bohumil Seifert Miluka kodová Fendrichová Jana pirková Viktor Voíek Stanislav Rejchrt TomáDouda Norbert Král Ilja Tachecí | 2012 | World Journal of Gastroenterology2012，18，32： | 8 |
| 3 | Sonographicallyguided compression repair of pseudoaneurysms: further ex2perience from a single institution 显示文摘 | Eisenberg L Paulson EK Kliewer | 1999 | AJ R1999，173，6： | 1 |
| 4 | Multi-objective simulation-based evolutionary algorithm for an aircraft spare parts allocation problem显示文摘 | Loo H L Ek P C Suyan T | 2008 | European Journal of Operational Research2008，189，： | 1 |
| 5 | Upregula-tion of PD-1 expression on HIV-specific CD8 T cells leads to reversible immune dysfunction 显示文摘 | Trautmann L Janbazian L Chomont N Said EA Gimmig S Bessette B Boulassel MR Delwart E Sepulveda H Balderas RS Routy JP Haddad EK Sekaly RP | 2006 | Nat Med2006，12，10： | 1 |
| 6 | Management of thyroid dysfunction during pregnancy and postpartum : an Endo- crine Society clinical practice guideline 显示文摘 | De Groot L Abalovich M Alexander EK | 2012 | J Clin Endocrinol Metab2012，97，8： | 1 |
| 7 | 显示文摘 | Helen L Gerhard J | 1998 | Chest1998，114，： | 1 |
| 8 | Sequence analysis and regu-lation of a gene encoding a cuticle-degrading serine protease from the nematophagous fungus Arthrobotrys oligospora显示文摘 | AHMAN J EK B RASK L | 1996 | Microbiology1996，142，7： | 1 |
| 9 | Probiotic Lactobacillusrhamnosus GG enhances gastric ulcer healing in rats显示文摘 | Lam EK Yu L Wong HP | 2007 | EuropeanJournal of Pharmacology2007，565，13： | 1 |
| 10 | Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa buckwheat and wheat as affected by sprouting and baking显示文摘 | ALVAREZ-JUBETE L WIJNGAARD H ARENDT EK | 2010 | Food Chemistry2010，119，2： | 1 |
| 11 | Results of interstitial brachytherapy for manignant brain tumors显示文摘 | Crockeer L Butker EK | 2002 | Inter J Oncol2002，21，4： | 1 |
| 12 | In situ structural evolution of arc-deposited Cr-based coatings显示文摘 | Neves A M Severo V Cvr(c)ek L | 2008 | Surface & Coatings Technology2008，202，55： | 1 |
| 13 | Multi-objective simulation-based evolutionary algorithm for an aircraft spare parts allocation problem 显示文摘 | Loo H L Ek P C Suyan T | | European Journal of Operational Research0，200，189： | 1 |
| 14 | Cardiovascular risk prediction in the general population with use of suPAR, CRP, and Framingham Risk Score 显示文摘 | Lyngb~ek S Marott J L Sehestedt T | 2013 | International journal of cardiology2013，167，6： | 1 |
| 15 | Neural stem-like cell line derived from a nonhematopoietic population of human umbilical cord blood显示文摘 | Buzanska L Marchinj EK Stachowiak M | 2006 | Stem Cells and Development2006，15，： | 1 |
| 16 | Human cord blood-derived cells attain neuronal and glial features in vitro显示文摘 | Buzanska L Machaj EK Zablocka B | 2002 | J cell Sci2002，115，10： | 1 |
| 17 | Melanin-concentrating hormone is a critical mediator of the leptin-deficient phenotype显示文摘 | Gabriella S L Richard L Bradley EK | 2003 | PNAS2003，100，10： | 1 |
| 18 | Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines: 2010 revision显示文摘 | Bro ek J L Bousquet J Baena-Cagnani C E Bonini S Canonica G W Casale T B | 2010 | J Allergy Clin Immunol2010，126，： | 1 |
| 19 | VIS absorption spectrophotometry of disperse dyes 显示文摘 | GOLOB V EK L T | 1999 | Dyes and Pigments1999，，40： | 1 |
| 20 | Effect of surfactants and polymers on stability and antibacterial activity of silver nanoparticles (NPs)显示文摘 | Kvítek L Paná(c)ek A Soukupová J | 2008 | Journal of Chemical Physics C2008，112，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员