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| 1 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 2 | RILL EROSION PROCESS ON STEEP SLOPE LAND OF THE LOESS PLATEAU显示文摘Rill erosion processes and principle of preventing rill erosion of different applied measures are studied by rainfall simulation and field investigation. Research results show that rill erosion processes can be schematically divided into five stages, that is, concentrated flow forming process; little waterfalls in the concentrated flow forming Process; little waterfalls developing into rill head cut process; rill head cuts developing to form discontinuous rill process and discontinuous rills connecting to form continuous rill process. Rill head cuts backward erosion, rill wall collapsing and runoff washing rill bed are the main patterns of rill development. The relevant equation between critical runoff volume of occurring rill erosion and slope degree can be expressed as follows: Q = 76. 10 - 2. 10J. And relevant equation between critical slope length of occurrence Of rill erosion and slope degree is parabolic with a minimum-value. In addition, influence of rill erosion development on slope runoff and soil erosion, benefits and principle of reducing rill erosion amount of mulch, non-tillage system and remained residue, strip alternation of grass and crop, horizontal ditch are also studied. | ZHENG Fenli and TANG Keli (1 The English text has been altered and edited by Dr. DING Lianzhen 2 Engineer, National Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Chinese Academy of Sciences and institute of Soil and Water Conservati | 1997 | International Journal of Sediment Research1997，12，1： | 9 |
| 3 | Amygdala responses to emotionally valenced stimuli in older and younger adults 显示文摘 | MATHER M CANLI T ENGLISH 1 | 2004 | Psychological Science2004，15，4： | 1 |
| 4 | SEDIMENT TRANSPORT MODEL OF A WATERSHED显示文摘The natural watershed is simply represented by a multi-order river net system, so the sediment transport process in watershed can be considered as sediment transport in the system. Based on this assumption, a new sediment graph model-Inverse Gaussian distribution instantaneous unit graph model is introduced in this paper. Then, the parameter estimation and other specific computational procedure are discussed. With the verification of 27 flood events measured in Chabagou watershed (in hilly and gullied areas of the Loess Plateau, Northern Shaanxi), the results are satisfactory and the model has a distinguishing feature in flexibility, model structure, and parameter numbers (only two). In addition, the phase difference between measured and simulated sediment flux are presented. and satisfactory results are obtained. | LI Huaien (1 The English text is edited by Dr. DING Lianzhen. 2 Associate professor, Xian University of Technology, Xian 710048, P. R. China) | 1997 | International Journal of Sediment Research1997，12，1： | 0 |
| 5 | ADVANCES IN MEASURING TECHNIQUES OF BED LOAD显示文摘Bed load is an important component of total load transportation. The accuracy of bed load measurement depends on the measuring equipment and sampling method. In this paper, the present situation of bed load measurement is introduced, the structure features and the sampling performance of bed lead samplers currently used in the world is evaluated. Results of field comparison of HS and TR2 type samplers used in USA, Don type sampler used in former USSR, and Chinese made Y78-1, Y80, AWT160, MB2 samplers have been summarized. From statistical point of view, selection of sampling verticals, and duration and number of repetitions in a vertical in the bed load measurement were studied. The error might be minimized if the sampling verticals were selected on the basis of equal partial bed load discharge. Finally, some data processing methods are recommended, which have been applied to the Changjiang River. | ZHOU Gangyan ZOU Jiazhong (1 The English text is edited and altered by Mr. Long Yuqian. 2 Senior engineer, Hydrology Bureau, Changjiang Water Resources Comm., Wuhan 430010, P. R. China. 3 Senior engineer (High grade), Hydrology Bureau, Changjiang Water Re | 1997 | International Journal of Sediment Research1997，12，1： | 0 |
| 6 | EFFECT OF SUSPENDED SEDIMENT ON THE TRANSPORT AND TRANSFORMATION OF 4 - NITROCHLOROBENZENE IN THE YELLOW RIVER显示文摘A simulation study of 4 - nitrochlorobenzene (P- NCB ) in the Yellow River has been per- formed. Parameters include partition on suspended sediment, volatility, and photolysis of pNCB in natural water body. The models consist of water hydrodynamics, DOC deoxygenation, and P-NCB water quality submodels. The results show different effects at different concentrations of suspended sediment and concentration of total organic carbon(TOC). | SHEN Xianchen (1 The English text is edited by Dr. DING Lianzhen 2 Senior Engineer, Water Quality Research Centre, China institute of Water Resources and Hydroelectric Power Research, Beijing 100044, P. R. China) | 1997 | International Journal of Sediment Research1997，12，1： | 0 |
| 7 | Negation and Semantic Interpretation of Wh-phrases显示文摘Chinesewh-phrasesareintriguingbecausetheirsemanticinterpretationsvaryaccordingtotheenvironmentsinwhichtheyappear.TheseaffectivecontextswhichlicenseanindefinitereadingofawhphrasehavebeeninvestigatedbyHuang(1982),Cheng(1991),Li(1992),Lin(1996,1998)amon... | Correspondence: 1) Wu Yaqing Faculty of English Language and Culture,Changsha Railway University,Changsha, Hunan, P.R. China 410075.2) wu inqing,Faculty of English language and Culture,Guangdong University of Foreign Studies,Guangzhou,P.R. China 501040 | 1999 | 现代外语1999，22，4： | 0 |
| 8 | Relationship between Sudden Sensorineural Hearing Loss and Vascular Risk Factors显示文摘Objective To explore the relationship between sudden sensorineural hearing loss(SSNHL) and vascular risk factors(including serum lipids and uric acid).Method This is a retrospective analysis of 100 cases of SSNHL seen at the Drum Tower Hospital,Nanjing Medical University,between Jan.2007 and Apr.2008。 Patient history,blood test results and imaging scans were analyzed.Levels of triglyceride(TG),cholesterols(CHO),high density lipoprotein-cholesterol(HDL-CH),low density lipoprotein-cholesterol(LDL-CH),apolipoprotein AI(ApoAI),apolipoprotein B(ApoB) and uric acid(UA) from these patients were compared with a control group of 56 patients treated for vocal cord polyps or nasal septum deviation during the same period.Patients with hypertension,diabetes,heart,brain,liver or kidney disorders are excluded from the present investigation.Results HDLCH level was higher and UA level lower in the study group than the control group(P < 0.05).HDL-CH and UA showed no significant differences among different age-groups(P > 0.05).There were no significant differences in the levels of TG,CHO,LDL-CH,ApoAI and ApoB(P > 0.05).Conclusion These data indicate that metabolic disturbances of serum lipids and/or uric acid may be potential risk factors for | ZHU Wen-yan1,DAI Yan-hong1,SHE Wandong1,WANG Lu2,Gao Xia1 1 Department of Otolaryngology-Head and Neck Surgery,Drum Tower Clinical Medical School,Nanjing Medical University,Nanjing 210008,China 2 Department of Medical English,School of Foreign Language,Nanfang Medical University,Guangzhou 510510,China | 2009 | Journal of Otology2009，4，1： | 0 |
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