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| 1 | Efficient Silencing of Endogenous MicroRNAs Using Artificial MicroRNAs in Arabidopsis thaliana显示文摘我们这里报导内长的 microRNAs (miRNAs ) 的表示能是高效地在用人工的 miRNA (amiRNA ) 的 Arabidopsis thaliana (Arabidopsis ) 的 silenced 技术。我们证明设计指向成熟 miRNA 的 amiRNA 对所有 miRNA 家庭成员指导 silencing,而设计指向一个 miRNA 先锋抄本的茎环区域的 amiRNA 对指向的仅仅单个家庭成员指导 silencing。而且,我们的结果显示指向成熟 miRNA 和茎环顺序的 amiRNAs 通过 miRNA 先锋抄本的劈开指导 RNA silencing,它大概在 miRNA 生物的续生说的起始的阶段期间发生在一个植物房间的原子核。这建议小 RNA (sRNA ) 指导了 RNA 在植物的劈开不仅在细胞质,而且在原子核发生。许多植物 miRNA 基因家庭经由定序和生物信息的分析被识别了,,但是,迄今为止,仅仅这些的小 tranche 机能上地由于有效前面或反向的基因工具的缺乏被描绘了。我们的调查结果因此在植物为 miRNA 功能的分析提供一个新、强大的颠倒基因的工具。 | Andrew L. Eamens Claire Agius Nell A. Smith Peter M. Waterhouse Ming-Bo Wang | 2011 | Molecular Plant2011，4，1： | 10 |
| 2 | Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice显示文摘The mammalian epididymis not only plays a fun dame ntal role in the maturati on of spermatozoa,but also provides protecti on agai nst various stressors.The foremost among these is the threat posed by oxidative stress,which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids,proteins,and nucleic acids.In mice,the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5(GPX5)as a major luminal scavenger in the proximal caput epididymidal segment.Accordingly,the loss of GPX5^-/-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx57 mice.To explore the underlying mechanism,we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged(13 months old)Gpx5^-/-m mice.This analysis revealed the dysregulation of several thousand epididymal mRNA transcripts,in eluding the downregulation of a subgroup of piRNA pathway gen es,in aged Gpx5^-/-mice.In agreeme nt with these fin dings,we also observed the loss of piRNAs,which potentially bind to the P-element-induced wimpy testis(PlWI)-like proteins PIWIL1 and PIWIL2.The absence of these piRNAs was correlated with the elevated mRNA levels of their putative gene targets in the caput epididymidis of Gpx5^-/-mice.Importantly,the oxidative stress response genes tend to have more targeting piRNAs,and many of them were among the top increased genes upon the loss of GPX5^-/-.Taken together,our findings suggest the existence of a previously uncharacterized somatic piRNA pathway in the mammalian epididymis and its possible invoIvement in the aging and oxidative stress-mediated responses. | Chen Chu Lu Yu Joelle Henry-Berger Yan-Fei Ru Ayhan Kocer Alexandre Champroux Zhi-Tong Li Miao He Sheng-Song Xie Wu-Bin Ma Min-Jie Ni Zi-Mei Ni Yun-Li Guo Zhao-Liang Fei Lan-Tao Gou Qiang Liu Samanta Sharma Yu Zhou Mo-Fang Liu Charlie Degui Chen Andrew L Eamens Brett Nixon Yu-Chuan Zhou Joel R Drevet Yong-Lian Zhang | 2020 | Asian Journal of Andrology2020，22，6： | 2 |
| 3 | Evaluation of a pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without mycobacterium paratuberculosis infection显示文摘 | S Gumber a G Eamens b R J Whittington a | 2006 | Veterinary Microbiology2006，115，： | 1 |
| 4 | Electronic absorption and fluorescence of new tetrafluoro-pentenyl-perylene in isotropic solvents,liquid crystal layers,and LB films显示文摘 | Wolarz Eamen Mykowska Erlou Marty(n)ski Tomes | | 0，，： | 1 |
| 5 | Evaluation of Erysipelothrix rhusiopathiae vaccines in pigs by intraderrnal challenge and im muneresponses显示文摘 | Eamens G Chin J Turner B | 2006 | Vet Mierohiol2006，116，13： | 1 |
| 6 | Charaeterisation of Erysipe lothricc rhusiopathiae isolates from pigs associated with vaccine breakdowns显示文摘 | Eamens G Forbes W Djordjevic S | 2006 | Vet Microbiol2006，115，4： | 1 |
| 7 | Characterisation of Erysipelothrix rhusiopathiae isolates from pigs associated with vaccine breakdowns显示文摘 | EAMENS G J FORBES W A | 2006 | Vet Microbiol2006，115，4： | 1 |
| 8 | Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apxIVA 显示文摘 | Schaller A Djordjevic S P Eamens G J | 2001 | Veterinary Microbiology2001，79，1： | 1 |
| 9 | Evaluation of Erysipelothrix Rhusiopathiae Vaccines in Pigs by Intradermal Challenge and Immune Responses显示文摘 | EAMENS G CHIN J TURNER B | | 0，，1： | 1 |
| 10 | An improved enzyme linked immunosorbent as-say(ELISA)for the detection of porcine serum anti-bodies against Mycoplasma hyopneumoniae显示文摘 | DJORDJEVIC S P EAMENS G J ROMALIS L F | 1994 | Vet Microbiol1994，39，34： | 1 |
| 11 | Identification of novel species-specific antigens of Mycoplasma hyopneumoniae by preparative SDS-PAGE ELISA profiling显示文摘 | SCARMAN A L CHIN J C EAMENS G J | 1997 | Microbiology1997，143，2： | 1 |
| 12 | Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apx IV A 显示文摘 | SCHALLER A DJORDJEVIE S P EAMENS G J | 2001 | Vet Microbiology2001，，79： | 1 |
| 13 | Molecular Characterization of a Ribonucleotide Reductase(nrdF) Gene Fragment of Mycoplasma hyopneumoniae and Assessment of the Recombinant Product as an Experimental Vaccine for Enzootic pneumonia 显示文摘 | Fagan PK Djordjevic SP Eamens GJ | 1996 | Infection and Immunity1996，64，： | 1 |
| 14 | Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene ApxⅣA显示文摘 | Schaller A Djordjevic S P Eamens G J | 2001 | Vet Microbiol2001，79，1： | 1 |
| 15 | Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasrna hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant显示文摘 | DJORDJEVIC S EAMENS G ROMALIS L | 1997 | Aust Vet J1997，75，： | 1 |
| 16 | Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apxIVA 显示文摘 | Schaller A Djordjevic S P Eamens G J | 2001 | Vet Microbiol2001，79，： | 1 |
| 17 | Identification and detection of Actinobacillus Pleuropleumoniae by PCR based on gene apx ⅣA显示文摘 | Schaller A Djordjevic S P Eamens G J | 2001 | Vet Microbiol2001，79，： | 1 |
| 18 | Evaluation of clinical, histological and immunological changes and qPCR detection of Mycoplasma hyopneumoniae in tissues during the early stages of mycoplasmal pneumonia in pigs after experimental challenge with two field isolates显示文摘 | Lauren K. Woolley Shayne Fell Jocelyn R. Gonsalves Mark J. Walker Steven P. Djordjevic Cheryl Jenkins Graeme J. Eamens | 2012 | 2012 (1-2)2012，，1： | 1 |
| 19 | Identification and detection of Actinobacillus pleurop neumoniae by PCR based on the gene ApxⅣ'A显示文摘 | Schaller A Djordejevic SP Eamens GJ | 2001 | Vet Microbiol2001，79，1： | 1 |
| 20 | Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apxIVA显示文摘 | Schaller A Djordjevie S P Eamens G J | 2001 | Vet Microbiol2001，79，1： | 1 |
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