|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | Pseudomelanosis duodeni associated with chronic renal failure显示文摘Pseudomelanosis duodeni (PD) is a rare dark speckled appearance of the duodenum associated with gastrointestinal bleeding,hypertension,chronic heart failure,chronic renal failure and consumption of different drugs.We report four cases of PD associated with chronic renal failure admitted to the gastroenterology outpatient unit due to epigastric pain,nausea,melena and progressive reduction of hemoglobin index.Gastroduodenal endoscopy revealed erosions in the esophagus and stomach,with no active bleeding at the moment.In addition,the duodenal mucosa presented marked signs of melanosis;later confirmed by histopathological study.Even though PD is usually regarded as a benign condition,its pathogenesis and clinical significance is yet to be defined. | Marcia Henriques de Magalhes Costa Maria da Gloria Fernandes Pegado Cleber Vargas Maria Elizabeth C Castro Kalil Madi Tiago Nunes Cyrla Zaltman | 2012 | World Journal of Gastroenterology2012，18，12： | 2 |
| 3 | 动态文化为骨头工程作为脚手架在 freeze-dried 骨头上改进 MSC 粘附显示文摘 AIM:To investigate the interaction between mesenchymal stem cells(MSCs) and bone grafts using two different cultivation methods:static and dynamic.METHODS:MSCs were isolated from rat bone marrow.MSC culture was analyzed according to the morphology,cell differentiation potential,and surface molecular markers.Before cell culture,freeze-dried bone(FDB) was maintained in culture for 3 d in order to verify culture medium pH.MSCs were co-cultured with FDB using two different cultivation methods:static co-culture(two-dimensional) and dynamic co-culture(threedimensional).After 24 h of cultivation by dynamic or static methods,histological analysis of Cell adhesion on FDB was performed.Cell viability was assessed by the Trypan Blue exclusion method on days 0,3 and 6 after dynamic or static culture.Adherent cells were detached from FDB surface,stained with Trypan Blue,and quantified to determine whether the cells remained on the graft surface in prolonged non-dynamic culture.Statistical analyses were performed with SPSS and a P < 0.05 was considered significant.RESULTS:The results showed a clear potential for adipogenic and osteogenic differentiation of MSC cultures.Rat MSCs were positive for CD44,CD90 and CD29 and negative for CD34,CD45 and CD11bc.FDBs were maintained in culture for 3 d and the results showed there was no significant variation in the culture medium pH with FDB compared to pure medium pH(P > 0.05).In histological analysis,there was a significant difference in the amount of adhered cells on FDB between the two cultivation methods(P < 0.05).The MSCs in the dynamic co-culture method demonstrated greater adhesion on the bone surface than in static co-culture method.On day 0,the cell viability in the dynamic system was significantly higher than in the static system(P < 0.05).There was a statistical difference in cell viability between days 0,3 and 6 after dynamic culture(P < 0.05).In static culture,cell viability on day 6 was significantly lower than on day 3 and 0(P < 0.05).CONCLUSION:An alternative cultivation method was developed to improve the MSCs adhesion on FDB,demonstrating that dynamic co-culture provides a superior environment over static conditions. | Fabiany da Costa Gonalves Ana Helena da Rosa Paz Priscila Schmidt Lora Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima | 2012 | World Journal of Stem Cells2012，4，2： | 2 |
| 4 | Surgical intervention in the setting of parenteral nutritiona-associated cholestasis may exacerbate liver injury显示文摘 | Arsenault DA Potemkin AK Elizabeth MR | | 0，，01： | 1 |
| 5 | Determ-ination of the Disulfide Bond Arrangement of Newcastle Disease Virus Hemagglutinin Neuraminidasc显示文摘 | James J Pitt Elizabeth Da Silva Jeffrey J | 2000 | The Journal of Biological Chemistry2000，275，9： | 1 |
| 6 | A transgenetic algorithm applied to the Traveling Car Renter Problem显示文摘 | Marco C. Goldbarg Elizabeth F.G. Goldbarg Paulo H. Asconavieta Matheus da S. Menezes Henrique P.L. Luna | 2013 | Expert Systems With Applications2013，，16： | 1 |
| 7 | A new technical option for domino liver transplantation显示文摘 | Lucio Filgueiras Pacheco-Moreira Marcelo Enne de Oliveira Elizabeth Balbi Alexandre Cerqueira da Silva Rosalice Miecznikowski Lucio José Auler de Faria Rodrigo Pereira Diaz André Jo?o Luiz Pereira Roberto Jamil Muharre José Manoel da Silva Gomes Martinho | 2003 | Liver Transplantation2003，，6： | 1 |
| 8 | Life skills and subiective well-being of people with disabilities:a canonical correlation analysis显示文摘 | Elizabeth da Silva Cardosel Kacie Blalock eds | 2004 | International Journal of Rehabilitation Researeh2004，27，4： | 1 |
| 9 | Development of the neuromuscular junction : genetic analysis in mice 显示文摘 | Joshua RS Elizabeth DA Robert WB | 1998 | J Physiol1998，92，1： | 1 |
| 10 | Incorporating endorectal MR elastography into multi‐parametric MRI for prostate cancer imaging: Initial feasibility in volunteers显示文摘 | Arvin Arani Michael Da Rosa Elizabeth Ramsay Don B. Plewes Masoom A. Haider Rajiv Chopra | 2013 | J. Magn. Reson. Imaging2013，，： | 1 |
| 11 | Effects of dietary fibers with different fermentation characteristics on feeding motivation in adult female pigs显示文摘 | Carol Souza da Silva J. Elizabeth Bolhuis Walter J.J. Gerrits Bas Kemp Joost J.G.C. van den Borne | 2013 | Physiology & Behavior2013，，： | 1 |
| 12 | Unregulated IL-23/IL-17 immune response in autoimmune diseases显示文摘 | Vinicius Silva Costa Teresa Cristina Colvara Mattana Maria Elizabeth Rossi da Silva | 2010 | Diabetes Research and Clinical Practice2010，，3： | 1 |
| 13 | Potential uses for lapachol in cosmetics显示文摘 | Franco Elizabeth Pires da Motta | 1986 | Aerosol Cosmet1986，8，48： | 1 |
| 14 | Growth and nutrient accumulation of Brazil nut trees(Bertholletia excelsa) in agroforestry at different fertilizer levels显示文摘The Brazil nut tree(Bertholletia excelsa) is a frequent component of agroforestry systems in the Amazon because of its adaptation to nutrient-poor upland soils and multiple uses.We investigated the aboveground biomass production(kg dry weight),nutrient uptake and requirements(N,P,Ca,Mg,K) of Brazil nut trees of different sizes grown under agroforestry conditions and fertilized at different levels.Eight of 70 experimental trees with different size were harvested and stem,branches and leaves were separated.Nutrient contents were determined for three trees of varying size.Average tree growth was fast,but variability was high,suggesting considerable potential for the improvement of this economically important species.The trees responded to increased levels of fertilizer and lime with significantly increased foliar nutrient contents and growth,probably because of the improved availability of Mg and Ca for which the species seems to have a relatively high demand.In contrast to Brazil nut trees grown in forest or dense plantations,the agroforestry trees invested a substantial part of their biomass and nutrients in large branches and developed spreading crowns.To improve stem form,reduce competition with associated crops for light and recycle nutrients,regular pruning of lower branches or planting arrangements that favor self-pruning are recommended.These measures would also increase the recycling of Ca and Mg,large quantities of which are contained in the branches. | Gotz Schroth Maria do Socorro Souza da Mota Maria Elizabeth de Assis Elias | 2015 | Journal of Forestry Research2015，26，2： | 0 |
| 15 | Analysis of the Power System from an Electron Beam Accelerator and the Correlation with the Theoretical Dosimetry for Radiation Processing显示文摘 | Samir Luiz Somessari Elizabeth S. Ribeiro Somessari Carlos Gaia da Silveira Wilson Aparecido Parejo Calvo | 2015 | Journal of Physical Science and Application2015，5，5： | 0 |
| 16 | Acute kidney injury in leptospirosis:Overview and perspectives显示文摘Leptospirosis is a bacterial disease disseminated through the centuries in the whole world which causes symptoms that go from self-limited diseases to hemorrhagic manifestations and organ failure, including acute kidney injury(AKI), composing the severe disease known as the Weil's syndrome. Mortality rates varies according to the clinical presentation and usually increases when kidney injury is present, and is even higher in the setting of pulmonary hemorrhage. There are recent advances in the search for novel biomarkers of renal involvement and early detection of AKI in leptospirosis, as well as in its pathophysiology. We review in this article the clinical aspects of leptospirosis-associated AKI and the perspectives for future research. | Geraldo Bezerra da Silva Junior Nattachai Srisawat Gabriela Studart Galdino ênio Sima Macedo JoséReginaldo Pinto Geysa Maria Nogueira Farias Renan Lima Alencar Roberto da Justa Pires Neto Elvino JoséGuardao Barros Elizabeth De Francesco Daher | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，10： | 0 |
| 17 | Facilitating adult learning and a researcher identity through a higher education pedagogical process显示文摘 | Lisa L. Wright Lange Elizabeth Da Costa Jose | 2009 | 美中教育评论2009，6，11： | 0 |
| 18 | Influences of Amazonian White and Black Waters on the Hematological and Biochemical Plasma Features of Arapaima gigas (Osteoglossiformes)显示文摘 | Cleverson Agner Ramos Jose Carlos Nunes Raulino Glauber Cruz de Menezes Iracimar Batista do Carmo Elenice Martins Brasil Elizabeth Gusmao Affonso Oscar Tadeu Ferreira da Costa Marisa Narciso Femandes | 2014 | Journal of Life Sciences2014，8，3： | 0 |
| 19 | The Spatial-Temporal Issue in Fiction:Finding the Actual World in Pu Songling’s Story显示文摘本文从西方现当代叙事学的角度探讨蒲松龄短篇小说《画壁》的空间处理问题,并通过空间与时间的互动,来分析聊斋人物的出场和在场状态,尤其是人物与故事发展的关系。这一分析聚焦于'空间等级'和'可能世界'的理论,涉及'空间边界''空间实体'与'可达空间'等当代文学批评概念。 | Elizabeth Familiar DA SILVA | 2014 | Comparative Literature:East & West2014，，1： | 0 |
| 20 | Clinical outcomes of hospitalized patients with chikungunya fever: A retrospective analysis显示文摘Objective:To describe the prognostic and clinical profile of hospitalized patients with chikungunya virus(CHIKV)infection focusing on renal outcomes.Methods:This is a cross-sectional study including all patients with confirmed chikungunya fever(CHIKF)admitted to 3 different highcomplexity hospitals in Fortaleza,Brazil between January 2016 and June 2017.Data analysis was carried out to evaluate correlation between clinical profile and outcomes.Results:Fifty-five patients were included,with a median age of 77(IQR=21)years,and 23(41.82%)were male.Twenty-five patients(45.45%,25/55)developed acute kidney injury(AKI),and 15(60.00%,15/25)were classified as KDIGO 1,1(4.00%)as KDIGO 2,and 9(36.00%)as KDIGO 3.The overall mortality was 34.54%whilst AKI-related mortality was 64.00%(16/25).Both AKI and encephalitis were associated with higher mortality.Patients who died were significantly older[82(IQR=12)years vs.70(IQR=28.75)years,P<0.001].In the multivariate analysis,abdominal pain was associated with an increased risk of severe AKI(OR=5.33,95%CI=1.11–25.64,P=0.037)and AKI was an independent risk factor of death(OR=12.06,95%CI=2.55–57.15,P=0.002).Recovery of renal function was similar among the different age groups.Conclusions:AKI is present in half of the study population and is an independent risk factor of death.Thus,renal function should be carefully monitored in hospitalized patients with CHIKV infection. | Lucas Lobo Mesquita Enio Simas Macedo Sergio Luiz Arruda Parente Filho Francisca Lillyan Christyan Nunes Beserra Evelyne Santana Girao Juliana Mandato Ferragut Roberto da Justa Pires Neto Geraldo Bezerra da Silva Junior Elizabeth De Francesco Daher | 2021 | Asian Pacific Journal of Tropical Medicine2021，14，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员