1篇
您的检索式：作者名="Eric AWong"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Nutritional stimulation by in-ovo feeding modulates cellular proliferation and differentiation in the small intestinal epithelium of chicks显示文摘Nutritional stimulation of the developing small intestine of chick embryos can be conducted by in-ovo feeding(IOF).We hypothesized that IOF of glutamine and leucine can enhance small intestinal development by promoting proliferation and differentiation of multipotent small intestinal epithelial cells.Broiler embryos(n=128)were subject to IOF of glutamine(IOF-Gln),leucine(IOF-Leu),NaCl(IOF-NaCl)or no injection(control)at embryonic d 17(E 17).Multipotent,progenitor and differentiated cells were located and quantified in the small intestinal epithelium between E 17 and d 7 after hatch(D 7)in all treatment groups by immunofluorescence of SRY-box transcription factor 9(Sox9)and proliferating cell nuclear antigen(PCNA),in-situ hybridization of leucine-rich repeat containing G-protein coupled receptor 5(Lgr5)and peptide transporter 1(PepT1)and histochemical goblet cell staining.The effects of IOF treatments at E 19(48 h post-IOF),in comparison to control embryos,were as follows:total cell counts increased by 40%,33%and 19%,and multipotent cell counts increased by 52%,50%and 38%,in IOF-Gln,IOF-Leu and IOF-NaCl embryos,respectively.Only IOF-Gln embryos exhibited a significance,36%increase in progenitor cell counts.All IOF treatments shifted Lgr5+ stem cell localizations to villus bottoms.The differentiated,PepT1+region of the villi was 1.9 and 1.3-fold longer in IOF-Gln and IOF-Leu embryos,respectively,while goblet cell densities decreased by 20%in IOF-Gln embryos.Postehatch,crypt and villi epithelial cell counts were significantly higher IOF-Gln chicks,compared to control chicks(P<0.05).We conclude IOF of glutamine stimulates small intestinal maturation and functionality during the peri-hatch period by promoting multipotent cell proliferation and differentiation,resulting in enhanced compartmentalization of multipotent and differentiated cell niches and expansions of the absorptive surface area. | Naama Reicher Tal Melkman-Zehavi Jonathan Dayan Eric AWong Zehava Uni | 2022 | Animal Nutrition2022，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员