|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Identification and characterization of GLOBE, a major gene controlling fruit shape and impacting fruit size and marketability in tomato显示文摘Within large-fruited germplasm,fruit size is influenced by flat and globe shapes.Whereas flat fruits are smaller and retain better marketability,globe fruits are larger and more prone to cuticle disorders.Commercial hybrids are often developed from crosses between flat and globe shaped parents because flat shape is thought to be dominant and fruit size intermediate.The objectives of this study were to determine the genetic basis of flat/globe fruit shape in large-fruited fresh-market tomato germplasm and to characterize its effects on several fruit traits.Twenty-three advanced single plant selections from the Fla.8000×Fla.8111B cross were selectively genotyped using a genome-wide SNP array,and inclusive composite interval mapping identified a single locus on the upper arm of chromosome 12 associated with shape,which we termed globe.A 238-plant F 2 population and 69 recombinant inbred lines for this region from the same parents delimited globe to approximately 392-kilobases.A germplasm survey representing materials from multiple breeding programs demonstrated that the locus explains the flat/globe shape broadly.A single base insertion in an exon of Solyc12g006860,a gene annotated as a brassinosteroid hydroxylase,segregated completely with shape in all populations tested.CRISPR/Cas9 knock out plants confirmed this gene as underlying the globe locus.In silico analysis of the mutant allele of GLOBE among 595 wild and domesticated accessions suggested that the allele arose very late in the domestication process.Fruit measurements in three genetic backgrounds evidenced that globe impacts fruit size and several fruit shape attributes,pedicel length/width,and susceptibility of fruit to weather check.The mutant allele of GLOBE appears mostly recessive for all traits except fruit size where it acts additively. | Edgar Sierra-Orozco Reza Shekasteband Eudald Illa-Berenguer Ashley Snouffer Esther van der Knaap Tong Geon Lee Samuel F.Hutton | 2021 | Horticulture Research2021，8，1： | 2 |
| 2 | Characterization of modified mesoporous silica nanoparticles as vectors for siRNA delivery显示文摘Gene therapy using siRNA molecules is nowadays considered as a promising approach. For successful therapy, development of a stable and reliable vector for siRNA is crucial. Non-viral and non-organic vectors like mesoporous silica nanoparticles(MSN) are associated with lack of most viral vector drawbacks, such as toxicity, immunogenicity, but also generally a low nucleic acid carrying capacity. To overcome this hurdle, we here modified the pore walls of MSNs with surface-hyperbranching polymerized poly(ethyleneimine)(hbPEI), which provides an abundance of amino-groups for loading of a larger amount of siRNA molecules via electrostatic adsorption. After loading, the particles were covered with a second layer of pre-polymerized PEI to provide better protection of siRNA inside the pores, more effective cellular uptake and endosomal escape. To test the transfection efficiency of PEI covered si RNA/MSNs, MDA-MB 231 breast cancer cells stably expressing GFP were used. We demonstrate that PEI-coated si RNA/MSN complexes provide more effective delivery of si RNAs compared to unmodified MSNs. Thus, it can be concluded that appropriately surface-modified MSNs can be considered as prospective vectors for therapeutic siRNA delivery. | Anna Slita Anna Egorova Eudald Casals Anton Kiselev Jessica M.Rosenholm | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，6： | 2 |
| 3 | Distribution and potential toxicity of engineered inorganic nanoparticles and carbon nanostructures in biological systems显示文摘 | Eudald Casals Socorro Vázquez-Campos Neus G Bastús | 2008 | Trends Anal Chem2008，27，： | 1 |
| 4 | Evaluation of the ecotoxicity of model nanoparticles显示文摘 | Raquel Barrena Eudald Casals Joan Colón Xavier Font Antoni Sánchez Víctor Puntes | 2009 | Chemosphere2009，，7： | 1 |
| 5 | Effect of cerium dioxide, titanium dioxide, silver, and gold nanoparticles on the activity of microbial communities intended in wastewater treatment显示文摘 | Ana García Lucía Delgado Josep A. Torà Eudald Casals Edgar González Víctor Puntes Xavier Font Julián Carrera Antoni Sánchez | 2011 | Journal of Hazardous Materials2011，，： | 1 |
| 6 | 一种桔皮低成本培养细菌纤维素的研究显示文摘细菌纤维素(bacterial cellulose,简称BC)是由木醋杆菌(Gluconacetobacter xylinus)生产的一种具有高纯度、高持水能力、高机械强度、高结晶度和弹性的生物聚合物.本研究以木醋杆菌为菌株,在静态条件下培养3d,Hestrin-Schramm(HS)培养基中气-液界面产生干重为0.188g/20 mL的BC薄膜;分别使用桔皮培养基、桔皮和HS混合培养基、HS培养基培养BC薄膜,用SEM、TEM进行表征,结果表明,采用不同培养基培养的BC的形貌结构不会发生变化.除此之外,本研究还发现采用桔皮、甘蔗皮、杂草等废料作为培养基均可以产生BC,虽在相同的时间内,其产量低于HS培养基的产量,但不会改变BC的形貌结构.采用废果皮制备BC生产的培养基,可以实现废弃果皮的再利用、降低BC的生产成本、提高BC的实用性,在大规模生产中具有潜在价值. | 刘兴菲 林轶超 张宇萍 李小芳 梁丽华 曾牡玲 CASALS MERCADAL Eudald | 2023 | 五邑大学学报(自然科学版)2023，37，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员