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| 1 | A peroxisome proliferator response elements regulatory system in xenopus oocytes and its application显示文摘Background Peroxisome proliferator-activated receptor-gamma (PPARγ) is a kind of ligand-activated transcription factors binding to peroxisome proliferator response element (PPRE), a specific recognition site. It is thought to play a critical role in glucose and lipid metabolism and in inflammation control. The aim of this study was to establish a new cellular model for the quick screening of lipid-lowering drugs, which may be effective as PPAR-γ ligands on the PPRE-mediated pathway regulatory system. Methods Two plasmids were constructed: pXOE-PPARγ, in which the human PPARγ gene was in the downstream of TFⅢA gene promoter, and pLXRN-PPRE-d2EGFP, in which the enhanced green fluorescent protein (EGFP) gene was subcloned into PPRE. The xenopus oocytes were injected with these two plamids, and consequently treated with prostaglandin E1, pioglitazone, and different kinds of lipid-lowering drugs. After 3 days, the oocytes were observed under a fluorescence microscope. To confirm the drug action,we injected pXOE-PPARγ plasmid into the oocytes, which then treated with prostaglandin E1and Hawthorn flavonoids. The mass of expressed lipoprotein lipase (LPL) in the cells was determined by enzyme labeling linked immunosorbent assay (ELISA).Results The expression of EGFP was only induced by prostagalandin E1, pioglitazone, Hawthorn flavonoids. A concentration-response relationship was seen between expressed EGFP and Hawthorn flavonoids. The levels of LPL in both Hawthorn flavonoids groups and PPARγ ligand prostagalandin E1 group injected with pXOE-PPARγ plamid increased significantly (P<0.001) compared with controls, and a concentration-response relationship was observed between LPL mass and Hawthorn flavonoids. Conclusions It is possible to establish a PPRE regulatory EGFP reporter system in xenopus oocytes to monitor the activity of PPARγ ligand. Hawthorn flavonoids can increase the expression of gene downsteam of PPRE by effect on the PPRE pathway regulatory system. | YAN Jin FAN Chun-lei WO Xing-de GAO Li-ping | 2005 | Chinese Medical Journal2005，，16： | 2 |
| 2 | Effect of curcumin on the expression of LDL receptor in mouse macrophages显示文摘 | Chunlei Fan Xingde Wo Ying Qian Jin Yin Liping Gao | 2006 | J Ethnopharmacol2006，105，12： | 1 |
| 3 | Curcumin up-regulates LDL receptor expression via the sterol regulatory element pathway in HepG2 cells显示文摘 | Dou X Fan C Wo L | | 0，，11： | 1 |
| 4 | Effeet of cur- cumin the Gene Expression of Low Density Lipoprotein Receptors显示文摘 | Fan Chunlei Qian Ying Wo Xingde | 2005 | Chin J Integer Med2005，11，3： | 1 |
| 5 | Effect of curcumin on the expression of LDL receptor in mouse macrophages显示文摘 | Fan C Wo X Qian Y | | 0，，1： | 1 |
| 6 | Effect of curcumin on the expression of LDL receptor in mouse macrophages显示文摘 | FAN C L WO X D QIAN Y | 2006 | J Ethnopharmacol2006，105，12： | 1 |
| 7 | Curcumin upregulates LDL receptor expression via sterol regulatory element pathway in HepG2 cells显示文摘 | Xiaobing Dou Chunlei Fan Like Wo | 2008 | Planta Med2008，74，： | 1 |
| 8 | Effect of curcumin on the expression of LDL receptor in mouse macrophages显示文摘 | Chunlei Fan Xingde Wo Ying Qian | 2006 | J Ethnopharmacol2006，105，12： | 1 |
| 9 | Biodistribution characteristics of ^(188)Re-TSC after TAE in rabbits bearing VX_2 liver tumor显示文摘188Re-tin sulfur colloid(TSC) was prepared to compare its biodistribution characteristics with 188Re-macroaggregates album(MAA) after transhepatic arterial embolization(TAE) in rabbits bearing VX2 liver tumor.Labeling efficiencies of the 188Re-TSC and 188Re-MAA were 99.94%±0.04% and 99.95%±0.03%,respectively,and they were stable for 72 h in human serum.Sintigraphy and biodistribution in 31 rabbits bearing VX2 liver tumor after transcatherter hepatic arterial injection of the radiopharmaceuticals were performed,and relevant activity accumulated mostly in the tumor.The percentage of the injected dose per gram of wet tissue(%ID/g) of 188Re-TSC and 188Re-MAA were calculated.Tumor uptake of 188Re-TSC at 1 h and 24 h were 24.32%±11.93% and 21.88%±18.29%,and the radioactive ratios of tumor/liver were 70.89±19.58 and 17.42±13.96,respectively.Tumor uptake of 188Re-MAA at 1 h and 24 h were 38.78%±30.23% and 15.98%±26.64%,and the radioactive ratio of tumor/liver of 188Re-MAA were 39.71±25.06 and 8.13±4.61,respectively.188Re-TSC is a potential radiopharmaceutical for the therapy of tumors. | ZHANG Bin WU Yiwei JIN Yonghai LIU Yizhi LUO Xianwen FAN Wo | 2009 | Nuclear Science and Techniques2009，20，2： | 1 |
| 10 | Curcumin up-regulates LDL receptor expression via the sterol regulatory element pathway in HepG2 cells显示文摘 | Dou X Fan C Wo L | 2008 | Planta Med2008，74，11： | 1 |
| 11 | Regulation of LDL receptor expression by the effect of eureumin on sterol regulatory element pathway显示文摘 | Fan C Wo X Dou X | 2006 | Pharmacol Rep2006，58，4： | 1 |
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