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| 1 | Initial study of biexponential model of intravoxel incoherent motion magnetic resonance imaging in evaluation of the liver fibrosis显示文摘 | Chen Cuiyun Wang Bin Shi Dapeng Fu Fangfang Zhang Jiliang Wen Zejun Zhu Shaocheng Xu Junling Lin Qing Li Jing Dou Shewei | 2014 | Chinese Medical Journal2014，，17： | 15 |
| 2 | Co_(78)Si_(8)B_(14)metallic glass:A highly efficient and ultra-sustainable Fenton-like catalyst in degrading wastewater under universal pH conditions显示文摘Overcoming the pH limitation and increasing the catalyst reusability remain pressing demands for metal-lic glass(MG)in wastewater remediation.Herein,Co_(78)Si_(8)B_(14)MG ribbons are used to degrade dye wastew-ater by activating hydrogen peroxide(H_(2)O_(2))as Fenton-like catalysts.The Co-based MG catalysts exhibit high degradation efficiency under both acidic and alkaline conditions,and the kinetic reaction rate at pH 10(0.176 min^(−1)) and pH 4(0.089 min^(−1)) is 5.9 and 1.2 times higher than that of the extensively studied Fe-based MG catalysts,respectively.Impressively,the Co-based MG catalysts can be reused up to 20-60 times at universal pH conditions,demonstrating fairly good reusability.The newly discovered Co-based MG catalysts do not follow the classical Fenton reactions with H_(2)O^(2) the way Fe-based MGs do.In an acid environment,hydroxyl radicals play a dominant role in the degradation,while in an alkaline environ-ment,the effect of hydroxyl radicals is weakened,and Co^(3+) ions exert a relatively major function on the degradation.The excellent performance in catalytic activity and reusability at universal pH conditions of the Co-based MGs will inspire the development of MGs in wastewater treatment. | Xindong Qin Jiliang Xu Zhengwang Zhu Zhengkun Li Dawei Fang Huameng Fu Shiming Zhang Haifeng Zhang | 2022 | Journal of Materials Science & Technology2022，，18： | 2 |
| 3 | PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome显示文摘Generation of transgenic mice by DNA microinjection has become one of the most important technologies in studying gene function in vivo. Ran- dom integration of transgene often results in inser- tional mutation which may complicate phenotype analysis of transgenic mice and/or create a good opportunity to study the function of endogenous gene. However,the utilization of these potentially valuable resources is hampered due to lack of efficient ap- proaches for rapid identification of multi-copy trans- gene integration sites. Here we propose two PCR-based approaches to identifying trans- gene/genome junction sequences. The efficiency of these two strategies was tested in 9 independent transgenic mouse lines. Among them,the transgene in 8 mouse lines was clearly localized to certain chromosome regions. These rapid and efficient ap- proaches will greatly facilitate the study of insertional mutation due to transgene integration. | ZHAO Xudong DANG Suying LIANG Bin LEI Xia CHEN Zheng WANG Long YAN Lanzhen SUN Hantang FU Jiliang FEI Jian WANG Zhugang | 2006 | Chinese Science Bulletin2006，51，18： | 2 |
| 4 | Research on Urban CommunitySport Service Management Mode of the Grids显示文摘 | Jiliang Fu Yongliang Huang | 2011 | Advances in Electrical Engineering and ElectricalMachines2011，，134： | 1 |
| 5 | Construction and application of gene targeting replacement vector formouse coagulation factor IX gene显示文摘Objective: To construct and apply a replacement targeting vector for mouse coagulation factor IX(mFIX) gene in embryonic stem (ES) cells. Methods: Based on the cloning and structural analysis of the genomic DNA fragment of coagulation factor IX gene from 129Sv mouse genomic DNA A phage library, PMFIXDEL plasmid was designed with positive-negative-selection (PNS) strategy, and constructed with commonmolecular cloning techniques. Structure of PMFIXDEL was identified by PCR and restriction analysis. Afterelectroporation with the linearized PMFIXDEL DNA, transfected ES cells were cultured in G418/GANC drugselection medium. The recombination efficacy of this vector was tested. Results: The main components ofPMFIXDEL were two copies of negative selection gene (HSV-tk expression cassette), a positive selectiongene (Neo expression cassette), long and short homologous fragments and plasmid backbone. The introduction of negative selection gene (HSV-tk ) into the construct resulted in 24-fold increase of selection.Conclusion: An effective replacement vector for mFIX gene targeting was constructed and applied in ES cell. | Dai Xuming’ Xue Hong Yang Hual Hu Yiping’ Fu Jiliang (Open Laboratory of Medical Molecular Genetics, Department of Basic Medicine Science, Second MilitaryMedical University, Shanghai, 200433)(Department of Biochemistry, hong Kong University of Scienc | 1998 | Journal of Medical Colleges of PLA(China)1998，13，2： | 0 |
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