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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Circulating ghrelin in pa-tients with gastric or colorectal cancer显示文摘 | Huang Q Fan YZ Ge BJ | 2007 | Dig Dis Sci2007，52，3： | 1 |
| 3 | Enhanced critical heat flux during quenching of extremely dilute aqueous colloidal suspensions with graphene oxide nanosheets显示文摘 | Zhang L YuZ T Li D Y Fan LW Zhu YZ Hong R H HuY C Fan J R Cen K F | 2013 | ASME Journal of Heat Transfer2013，135，05： | 1 |
| 4 | Quantification of the internal resistance distribution of microbial fuel cells显示文摘 | Fan YZ Sharbrough E Liu H | 2008 | Environ Sci Technol2008，42，21： | 1 |
| 5 | CS3,a major QTL for grain length and weight minor QTL for grain width and thicknees in rice,encodes a putative transmembrane protein显示文摘 | FAN OC XING YZ MAO H L | 2006 | Theor Appl Genet2006，6，： | 1 |
| 6 | Effects of 420-nm intense pulsed light in an acne animal model显示文摘 | Fan X Xing YZ Liu LH | 2013 | J Eur Acad Dermatol Venereol2013，27，9： | 1 |
| 7 | Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cell显示文摘 | Fan YZ Fu JY Zhao ZM el al | 2005 | World J Gastroentero2005，11，16： | 1 |
| 8 | Clinical observation on tuina plus warm needling moxibustion for lumbar disc herniation显示文摘 | Zhu WM Wu YC Fan YZ | 2012 | J Acupunct Tuina Sci2012，10，4： | 1 |
| 9 | Characterization of a Hy-perthe-rmo- stable Fe-superoxide dismutase from hot spring 显示文摘 | He YZ Fan KQ Jia C J | 2007 | Appl Mierobiol Biotechno12007，75，2： | 1 |
| 10 | Circadian expression of clock and screening of clock-controlled genes in peripheral lymphocytes of rat显示文摘 | Du YZ Fan SJ Meng QH | | 0，，04： | 1 |
| 11 | Chalk 5 encodes a vacuolar H+ -translocating pyrophosphatase influencing grain ehalki- ness in rice显示文摘 | Li YB Fan CC Xing YZ | 2014 | Nature Genetics2014，46，4： | 1 |
| 12 | A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas in vivo and in vitro显示文摘 | Sun W Fan YZ Zhang WZ | | Journal of Experimental & Clinical Cancer Research0，，： | 1 |
| 13 | A pilot histomorphology and hemody- namic of vasculogenic mimicry in gallbladder carcinomas in vivo and in vitro显示文摘 | Sun W Fan YZ Zhang WZ | 2011 | J Exp Clin Cancer Res2011，，30： | 1 |
| 14 | Carbon storage in the grasslands of China based on field measurements of above-and below-ground biomass显示文摘 | Fan JW Zhong HP Harris W Yu GR Wang SQ Hu ZM Yue YZ | | 0，，： | 1 |
| 15 | Dimethylphthalate esterdegradation by two plank tonicand immobilized bacte-rial consortia显示文摘 | Wang YY Fan YZ Gu JD | 2004 | Int Biodeter&Biodegr2004，53，： | 1 |
| 16 | Determination of clopidol residues in chicken tissues by liquid chromatography:collaborative study显示文摘 | Pang GF Cao YZ Fan CL | 2003 | J AOAC Int2003，86，4： | 1 |
| 17 | Effects of 42011m intense pulsed light in an ache animal model 显示文摘 | Fan X Xing YZ Lin LH | 2013 | J Eur Aead Dermatol Venereol2013，27，9： | 1 |
| 18 | Thymopentin (TP5), an immunomodulatory peptide, suppresses proliferation and induces differentiation in HL - 60 cells 显示文摘 | Fan YZ Chang H Yu Y | 2006 | Biochim Biophys Acta2006，1763，10： | 1 |
| 19 | Expressinn of arginyl-tRNA synthetase in rats with local cerebral ischemia 显示文摘 | Fu R Fan YZ Fan YC | 2014 | J Hua- zhong Univ Set Technolog Med Set2014，34，2： | 1 |
| 20 | Determination of clopidol residues in chicken tissues by high-performance liquid chromatography-mass spectrometry 显示文摘 | Cao YZ Fan CL | 2000 | J Chromatogr A2000，882，12： | 1 |
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