|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Relationship between telomerase activity and its subunit expression and inhibitory effect of antisense hTR on pancreatic carcinoma显示文摘AIM: To directly investigate the relationship between telomerase activity and its subunit expression and the inhibitory effect of antisense hTR on pancreatic carcinogenesis.METHODS: We examined the telomerase activity and its subunit expression by cell culture, polymerase chain reaction (PCR), PCR-silver staining, PCR-ELISA, DNA sequencing, MTT and flow cytometry methods.RESULTS: PCR-silver staining and PCR-ELISA methods had the same specificity and sensitivity as the TRAP method.Telomerase activity was detected in the extract of the 10th,20th and 30th passages of P3 cells,while it was absent in fibroblasts. Furthermore, after the 30th generation, the proliferation period of fibroblast cells was significantly prolonged. Telomerase activity and hTERTmRNA were detected in two pancreatic carcinoma cell lines, but were found to be negative in human fibroblast cells. Telomerase activity and hTERTmRNA were tested in pancreatic carcinoma specimens of 24 cases. The telomerase activity was positive in 21 of the 24 cases (87.5 %), and the hTERTmRNA in 20 cases (83.3 %). In adjacent normal tissues positive rates were both 12.5 %. There was a significant difference between the two groups. This indicated a significant correlation between the expression level of telomerase activity and histologic differentiation,metastasis and advanced clinical stage of pancreatic carcinoma. Our findings showed that the expressions of hTR and TP1mRNA were not correlated with the activity of telomerase but the expression of hTERTmRNA was. After treatment with PS-ODNs, telomerase activity in P3 cells weakened and the inhibiting effect became stronger with an increase in PS-ODNs concentration. There was a significant difference between different PS-ODN groups (P<0.05). Inhibition of telomerase activity occurred most significant with PS-ODN1.The results of the FCM test of pancreatic cancer P3 cells showed an increase in the apoptotic rate with increasing PS-ODN1 and PS-ODN2concentrations.CONCLUSION: The expression of telomerase activity has a significant relationship to carcinogenesis. A strong correlation exists between telomerase activity and hTERTmRNA expression. The up-regulation of hTERTmRNA expression may play a critical role in human carcinogenesis.The expression of telomerase activity and its subunit level in pancreatic carcinoma significantly correlate with the clinical stage of pancreatic carcinoma and hence, may be helpful in its diagnosis and prognosis. The anti-hTR complementary to the template region of hTR is sufficient to inhibit P3 cell telomerase activity and cell proliferation in vitro, and can lead to a profound induction of programmed cell death. | Jia-HuaZhou Hong-MeiZhang QuanChen Dong-DongHan FeiPei Li-ShanZhang De-TongYang | 2003 | World Journal of Gastroenterology2003，9，8： | 10 |
| 2 | Region-based template deformation and masking for eye-feature extraction and description显示文摘 | Deng Jyh-yuan Lai Feipei | 1997 | Pattern Recognition1997，30，3： | 1 |
| 3 | Region-based Template Deformation and Masking for Eye-feature Extraction and Description显示文摘 | Deng J Y Lai Feipei | 1997 | Pattem Recognition1997，30，3： | 1 |
| 4 | ID-based di gital msnature scheme on the elliptic curve cryptosystem显示文摘 | Huang Kuo Hsuan Lai Feipei | 2007 | Computer Standard & Interfaces2007，29，6： | 1 |
| 5 | YMDD variants of HBV DNA polymerase gene: Rapid detection and clinicopathoiogical analysis with long-term Iamivudine therapy after liver transplantation显示文摘AIM: To look for a rapid low-cost technique for the detection of HBV variants.METHODS: Two patients who underwent orthotopic liver transplantation (OLT) for HBV infection were treated with lamivudine (100 mg daily) and HBV infection recurred in the grafted livers. The patients were monitored intensively for liver enzymes, hepatitis B surface antigen (HBsAg) and HBV DNA in serum. Liver biopsy was performed regularly. HBV DNA in a conserved polymerase domain (the YMDD locus) was amplified from serum of each patient by PCR and sequenced. HBV genotypes were analyzed by restriction fragment length polymorphism (RFLP) of the PCR products generated from a fragment of the polymerase gene.RESULTS: YMDD wild-type HBV was detected in one patient by PCR-RFLP and DNA sequencing 19 mo after OLT, and YIDD mutant-type HBV in the other patient, 16 mo after OLT.CONCLUSION: PCR-RFLP assay is an accurate and simple method for genotyping lamivudine-resistant HBV variants. | FeiPei Jun-YuNing Jiang-FengYou Jing-PinYang JieZheng | 2005 | World Journal of Gastroenterology2005，11，18： | 1 |
| 6 | Region-based template deforma- tion and masking for eye-feature extraction and description 显示文摘 | DENG J Y LAI Feipei | 1997 | Pattern Recognition1997，30，3： | 1 |
| 7 | Region-based Template Deformation and Masking for Eye Feature Extraction and Description显示文摘 | Feipei Lai | 1997 | Pattern Recognition1997，30，3： | 1 |
| 8 | Access control in user hierarchy based on elliptic curve cryptosystem显示文摘 | YU Fangchung LEE Hsiu hui LAI Feipei | 2008 | Information Sciences2008，178，： | 1 |
| 9 | Novel cryptographic key assignment scheme for dynamic access control in a hierarchy显示文摘 | L Shen Tzer-Shyong Chen Feipei Lai | 1997 | IEICE Trans Fundamentals1997，80，： | 1 |
| 10 | Region-based template deformation and masking for eye-feature extraction and description 显示文摘 | Deng Jyh-Yuan Lai Feipei | 1997 | Pattern Recognition1997，30，3： | 1 |
| 11 | Access control in user hierarchy based on elliptic curve cryptosystem显示文摘 | YU Fang chung LEE Hsiu hui LAI Feipei | 2008 | Information Sciences2008，178，： | 1 |
| 12 | Silencing of a novel tumor metastasis suppressor gene LASS2/TMSG1 promotes invasion of prostate cancer cell in vitro through increase of vacuolar ATPase activity显示文摘 | XiaoyanXu JiangfengYou FeiPei | 2012 | J Cell Biochem2012，，7： | 1 |
| 13 | FOS:a funnel-based approach for optimal online traffic smoothing of live video 显示文摘 | Lin Jeng-wei Chang Ray-I Feipei Lai | 2006 | IEEE Transactions on Multimedia2006，8，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员