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您的检索式：作者名="Feixue Song"
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| 1 | DDR1 promotes LoVo cell proliferation by regulating energy metabolism显示文摘Cellular energy metabolism dysregulation is associated with colorectal cancer(CRC)development and progression.Discoidin domain receptor 1a(DDR1a),one of the five DDR1 isoforms,is closely related to cell proliferation,invasion,and apoptosis in various tumors.Whether it participates in cellular metabolic reprogramming and regulates CRC initiation and progression remains unclear.In this study,we compared the expression of DDR1 in CRC tissues and adjacent tissues from 126 postoperative CRC samples.Moreover,lentivirus-mediated DDR1a overexpression and knockdown were performed in LoVo cells,and cell viability and proliferation were determined by CCK-8 and BrdU assays,respectively.Oxygen consumption rate,extracellular acidification rate,and lactate production were used to determine the effect of DDR1a on metabolic reprogramming.Clinically,CRC patients with high DDR1 expression had poor differentiation and were at an advanced TNM stage.DDR1a promoted LoVo cell proliferation,mitochondrial function,and extracellular acidification.Moreover,DDR1a knockdown inhibited intracellular lactic acid production in LoVo cells,while a pyruvate kinase inhibitor(diamide,200μM)significantly reversed this progression.Taken together,our results reveal that DDR1 plays a crucial role in maintaining intracellular environment homeostasis through metabolic reprogramming. | Bin Xiong Zehui Xie Feixue Song Huiling Chen Xiaojuan Wang Zhengxu Jin Tiyun Han Yi Li Dekui Zhang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，5： | 1 |
| 2 | The Influence of IFN-α on Blood Plasmacytoid Dendritic Cell in Chronic Myeloid Leukaemia显示文摘OBJECTIVE To study the mechanism of IFN on CML.METHODS Samples of 15 CML patients and 10 healthy controlswere studied. The flow cytometry was performed to identifycirculating pDCs. The concentration of IFN-α in serum and that inthe supernatant of peripheral blood mononuclear cells (PBMCs)cultured after stimulation with CpG ODN2216 were examinedboth in CML patients and in the healthy controlsRESULTS There was significant reduction in the numberof circulating pDCs, serum concentration of IFN-α and thecapacity of IFN-α producing PBMCs in CML patients comparedwith those in healthy control individuals (P < 0.001). After theactive treatment with IFN-α and hydroxyurea, the quantity andfunction of pDCs were increased in stabilized patients, especiallythe function of pDCs in 2 patients achieving major cytogeneticresponse (MCR). The proportion and function of pDCs and theserum levels of IFN were inversely correlated with both WBC andage of the patients with CML, and positively correlated with thestate of the illness.CONCLUSION CML patients had a reduced number anddysfunction of circulating pDCs. The active treatment with IFN inCML patients may be related to the restoration of pDCs. | Chongyang Wu Liansheng Zhang Ye Chai Feixue Song Pengyun Zeng Lijuan Li Lingling Yue Bin Xiong | 2009 | Chinese Journal of Clinical Oncology2009，6，2： | 0 |
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