|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 3D bioactive composite scaffolds for bone tissue engineering显示文摘Bone is the second most commonly transplanted tissue worldwide,with over four million operations using bone grafts or bone substitute materials annually to treat bone defects.However,significant limitations affect current treatment options and clinical demand for bone grafts continues to rise due to conditions such as trauma,cancer,infection and arthritis.Developing bioactive three-dimensional(3D)scaffolds to support bone regeneration has therefore become a key area of focus within bone tissue engineering(BTE).A variety of materials and manufacturing methods including 3D printing have been used to create novel alternatives to traditional bone grafts.However,individual groups of materials including polymers,ceramics and hydrogels have been unable to fully replicate the properties of bone when used alone.Favourable material properties can be combined and bioactivity improved when groups of materials are used together in composite 3D scaffolds.This review will therefore consider the ideal properties of bioactive composite 3D scaffolds and examine recent use of polymers,hydrogels,metals,ceramics and bio-glasses in BTE.Scaffold fabrication methodology,mechanical performance,biocompatibility,bioactivity,and potential clinical translations will be discussed. | Gareth Turnbull Jon Clarke Frederic Picard Philip Riches Luanluan Jia Fengxuan Han Bin Li Wenmiao Shu | 2018 | Bioactive Materials2018，3，3： | 40 |
| 2 | Application of Improved PSO-LSSVM on Network Threat Detection显示文摘To solve the problem of the design of classifier in network threat detection, we conduct a simulation experiment for the parameters' optimal on least squares support vector machine (LSSVM) using the classic PSO algorithm, and the experiment shows that uneven distribution of the initial particle swarm exerts a great impact on the results of LSSVM algorithm's classification. This article proposes an improved PSO-LSSVM algorithm based on Divide-and-Conquer (DCPSO- LSSVM) to split the optimal domain where the parameters of LSSVM are in. It can achieve the purpose of distributing the initial particles uniformly. And using the idea of Divide-and-Conquer, it can split a big problem into multiple sub-problems, thus, completing problems' modularization Meanwhile, this paper introduces variation factors to make the particles escape from the local optimum. The results of experiment prove that DCPSO-LSSVM has better effect on classification of network threat detection compared with SVM and classic PSOLSSVM. | QI Fumin XIE Xiaoyao JING Fengxuan | 2013 | Wuhan University Journal of Natural Sciences2013，18，5： | 4 |
| 3 | Building Osteogenic Microenvironments with a Double-Network Composite Hydrogel for Bone Repair显示文摘The critical factor determining the in vivo effect of bone repair materials is the microenvironment,which greatly depends on their abilities to promote vascularization and bone formation.However,implant materials are far from ideal candidates for guiding bone regeneration due to their deficient angiogenic and osteogenic microenvironments.Herein,a double-network composite hydrogel combining vascular endothelial growth factor(VEGF)-mimetic peptide with hydroxyapatite(HA)precursor was developed to build an osteogenic microenvironment for bone repair.The hydrogel was prepared by mixing acrylatedβ-cyclodextrins and octacalcium phosphate(OCP),an HA precursor,with gelatin solution,followed by ultraviolet photo-crosslinking.To improve the angiogenic potential of the hydrogel,QK,a VEGF-mimicking peptide,was loaded in acrylatedβ-cyclodextrins.The QK-loaded hydrogel promoted tube formation of human umbilical vein endothelial cells and upregulated the expression of angiogenesis-related genes,such as Flt1,Kdr,and VEGF,in bone marrow mesenchymal stem cells.Moreover,QK could recruit bone marrow mesenchymal stem cells.Furthermore,OCP in the composite hydrogel could be transformed into HA and release calcium ions facilitating bone regeneration.The double-network composite hydrogel integrated QK and OCP showed obvious osteoinductive activity.The results of animal experiments showed that the composite hydrogel enhanced bone regeneration in skull defects of rats,due to perfect synergistic effects of QK and OCP on vascularized bone regeneration.In summary,improving the angiogenic and osteogenic microenvironments by our double-network composite hydrogel shows promising prospects for bone repair. | Jiaying Li Jinjin Ma Qian Feng En Xie Qingchen Meng Wenmiao Shu Junxi Wu Liming Bian Fengxuan Han Bin Li | 2023 | Research2023，，3： | 3 |
| 4 | Evaluation of probiotic yoghurt by the mixed culture with Lactobacillus plantarum A3显示文摘Lactic acid bacteria(LAB)fermentation is the simplest and safest way of food preservation,and the use of probiotics in yoghurt could provide dairy products with unique flavors,textures and health benefits.In this study,Lactobacillus bulgaricus,Streptococcus thermophilus,L.reuteri DSMZ 8533 and the potential probiotic strain L.plantarum A3 were used for the milk fermentation.Results found the texture properties such as hardness,consistency,and viscosity of the yoghurt were enhanced in the mixed culture condition.Furthermore,components like amino acid(leucine),vanilla(vanillin),C;(unsaturated fatty acids)were also accumulated in L.plantarum A3 fermented yoghut,which leads to the significant sensory profiling difference compared with the former plain yoghurt.All these results proved L.plantarum A3 is a potential probiotic stom winch could enhance the sensory and nutrition profiling of the fermented milk.Future work still needs to be done on the synergistic interaction between the traditional strains and the probiotics during the fermentation process. | Fengxuan Lang Jianzhu Wen Zhen Wu Daodong Pan Linjun Wang | 2022 | Food Science and Human Wellness2022，11，2： | 3 |
| 5 | TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair显示文摘Annulus fibrosus(AF)repair remains a challenge because of its limited self-healing ability.Endogenous repair strategies combining scaffolds and growth factors show great promise in AF repair.Although the unique and beneficial characteristics of decellularized extracellular matrix(ECM)in tissue repair have been demonstrated,the poor mechanical property of ECM hydrogels largely hinders their applications in tissue regeneration.In the present study,we combined polyethylene glycol diacrylate(PEGDA)and decellularized annulus fibrosus matrix(DAFM)to develop an injectable,photocurable hydrogel for AF repair.We found that the addition of PEGDA markedly improved the mechanical strength of DAFM hydrogels while maintaining their porous structure.Transforming growth factor-β1(TGF-β1)was further incorporated into PEGDA/DAFM hydrogels,and it could be continuously released from the hydrogel.The in vitro experiments showed that TGF-β1 facilitated the migration of AF cells.Furthermore,PEGDA/DAFM/TGF-β1 hydrogels supported the adhesion,proliferation,and increased ECM production of AF cells.In vivo repair performance of the hydrogels was assessed using a rat AF defect model.The results showed that the implantation of PEGDA/DAFM/TGF-β1 hydrogels effectively sealed the AF defect,prevented nucleus pulposus atrophy,retained disc height,and partially restored the biomechanical properties of disc.In addition,the implanted hydrogel was infiltrated by cells resembling AF cells and well integrated with adjacent AF tissue.In summary,findings from this study indicate that TGF-β1-supplemented DAFM hydrogels hold promise for AF repair. | Qiang Wei Dachuan Liu Genglei Chu Qifan Yu Zhao Liu Jiaying Li Qingchen Meng Weishan Wang Fengxuan Han Bin Li | 2023 | Bioactive Materials2023，，1： | 3 |
| 6 | Role of microsatellite instability in occurrence of colorectal carcinoma and its prognostic significance 显示文摘 | Yan xiaochu Chen yisheng Liu fengxuan | 1999 | J Med Coil PLA1999，13，2： | 1 |
| 7 | 显示文摘 | FENGXuan(冯垣) ZHANGTan(张潭) BIANLiu-jiao(边六交) | | 高等学校化学学报0，，： | 1 |
| 8 | Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF显示文摘 | Fengxuan Han Xiaoling Jia Dongdong Dai Xiaoling Yang Jin Zhao Yunhui Zhao Yubo Fan Xiaoyan Yuan | 2013 | Biomaterials2013，，30： | 1 |
| 9 | Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration显示文摘 | ZHANG Hong JIA Xiaoling HAN Fengxuan | 2013 | Biomaterials2013，34，9： | 1 |
| 10 | Three-dimensional biofabrication of nanosecond laser micromachined nanofibre meshes for tissue engineered scaffolds显示文摘There is a high demand for bespoke grafts to replace damaged or malformed bone and cartilage tissue.Three-dimensional(3D)printing offers a method of fabricating complex anatomical features of clinically relevant sizes.However,the construction of a scaffold to replicate the complex hierarchical structure of natural tissues remains challenging.This paper reports a novel biofabrication method that is capable of creating intricately designed structures of anatomically relevant dimensions.The beneficial properties of the electrospun fibre meshes can finally be realised in 3D rather than the current promising breakthroughs in two-dimensional(2D).The 3D model was created from commercially available computer-aided design software packages in order to slice the model down into many layers of slices,which were arrayed.These 2D slices with each layer of a defined pattern were laser cut,and then successfully assembled with varying thicknesses of 100μm or 200μm.It is demonstrated in this study that this new biofabrication technique can be used to reproduce very complex computer-aided design models into hierarchical constructs with micro and nano resolutions,where the clinically relevant sizes ranging from a simple cube of 20 mm dimension,to a more complex,50 mm-tall human ears were created.In-vitro cell-contact studies were also carried out to investigate the biocompatibility of this hierarchal structure.The cell viability on a micromachined electrospun polylactic-co-glycolic acid fibre mesh slice,where a range of hole diameters from 200μm to 500μm were laser cut in an array where cell confluence values of at least 85%were found at three weeks.Cells were also seeded onto a simpler stacked construct,albeit made with micromachined poly fibre mesh,where cells can be found to migrate through the stack better with collagen as bioadhesives.This new method for biofabricating hierarchical constructs can be further developed for tissue repair applications such as maxillofacial bone injury or nose/ear cartilage replacement in the future. | Ross HMcWilliam Wenlong Chang Zhao Liu Jiayuan Wang Fengxuan Han Richard ABlack Junxi Wu Xichun Luo Bin Li Wenmiao Shu | 2023 | Biomaterials Translational2023，4，2： | 0 |
| 11 | Mechanically conditioned cell sheets cultured on thermo-responsive surfaces promote bone regeneration显示文摘Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades.However,efficient harvest and handling of cell sheets remain challenging,including insufficient extracellular matrix content and poor mechanical strength.Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types.However,currently,there are no effective ways to apply mechanical loading to cell sheets.In this study,we prepared thermo-responsive elastomer substrates by grafting poly(N-isopropyl acrylamide)(PNIPAAm)to poly(dimethylsiloxane)(PDMS)surfaces.The effect of PNIPAAm grafting yields on cell behaviours was investigated to optimize surfaces suitable for cell sheet culturing and harvesting.Subsequently,MC3T3-E1 cells were cultured on the PDMS-g-PNIPAAm substrates under mechanical stimulation by cyclically stretching the substrates.Upon maturation,the cell sheets were harvested by lowering the temperature.We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning.Reverse transcription quantitative polymerase chain reaction and Western blot analyses further confirmed that the expression of osteogenic-specific genes and major matrix components were up-regulated.After implantation into the critical-sized calvarial defects of mice,the mechanically conditioned cell sheets significantly promoted new bone formation.Findings from this study reveal that thermo-responsive elastomer,together with mechanical conditioning,can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. | Gen Wang Zhangqin Yuan Li Yu Yingkang Yu Pinghui Zhou Genglei Chu Huan Wang Qianping Guo Caihong Zhu Fengxuan Han Song Chen Bin Li | 2023 | Biomaterials Translational2023，4，1： | 0 |
| 12 | Assessment of the Tidal Current Energy Resources and the Hydrodynamic Impacts of Energy Extraction at the PuHu Channel in Zhoushan Archipelago,China显示文摘An unstructured model FVCOM(The Unstructured Grid Finite Volume Community Ocean Model)with sink momentum term was applied to simulate the tidal current field in Zhoushan Archipelago,China,with focus on the region named PuHu Channel between Putuo Island and Hulu Island.The model was calibrated with several measurements in the channel,and the model perform-ance was validated.An examination of the spatial and temporal distributions of tidal energy resources based on the numerical simula-tion revealed that the greatest power density of tidal energy during spring tide is 3.6kWm^(−2)at the northern area of the channel.Two parameters were introduced to characterize the generation duration of the tidal array that causes the temporal variation of tidal current energy.The annual average available energy in the channel was found to be approximately 2.6MW.The annual generating hours at rated power was found to be 1800 h when the installed capacity of tidal array is approximately 12MW.A site for the tidal array with 25 turbines was selected,and the layout of the array was configured based on the EMEC specifications.Hydrodynamic influence due to the deployment of the tidal array was simulated by the modified FVCOM model.The simulation showed that the tidal level did not significantly change because of the operation of the tidal array.The velocity reduction covered a 2km^(2)area of the downstream the tidal array,with a maximum velocity reduction of 8cms−1 at mid-flood tide,whereas the streamwise velocity on both sides of the farm increased slightly. | WU He YU Huaming FANG Yizhou ZHOU Qingwei ZHUO Fengxuan KELLY Ryan M. | 2021 | Journal of Ocean University of China2021，20，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员