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| 1 | Cy5.5-MSA-G250 nanoparticles(CMGNPs)induce M1 polarity of RAW264. 7 macrophage cells via TLR4-dependent manner显示文摘Cy5.5-MSA-G250 nanoparticles(CMGNPs)had been proved to have unique advantages for cancer treatment,including excellent photothermal performance,tumor cell-selective cytotoxicity,direct visualization,and good biocompatibility.However,to cellular systems,the CMGNPs are considered as fo reign invaders,and the effect of CMGNPs on immunity system is still unknown.Therefore,more efforts are needed to understand the role of CMGNPs on the immunity system.In this study,we attempted to screen the pro-inflammatory responses on RAW264.7 macrophages after treated with the CMGNPs.In vitro experiments clearly showed that CMGNPs not only enhances phagocytosis capacity of RAW264.7 cells,but also promotes Ml polarization,associated with changes in cell morphology and increased expression of inflammatory cytokines.This ability to induce Ml polarization may be beneficial to CMGNPs to achieve better anticancer effects in clinical trials.Moreover,the observed Ml macrophages’ polarization triggered by CMGNPs can be abolished after adding TLR4 inhibitor,CLI095,suggesting that TLR4 is involved in CMGNP-induced inflammation. | Zhuoxuan Lu Lingfeng Xu Nongyue He Fengying Huang Tiefeng Xu Li Li Yanwei Zhang Liming Zhang | 2019 | Chinese Chemical Letters2019，30，6： | 2 |
| 2 | 查看详情显示文摘 | Chang C Z Zhang J Feng X Shen J Zhang Z C Guo MH LiK OuYB WeiP WangLL JiZQ FengY Ji S H Chen X Jia J F Dai X Fang Z Zhang S C He K Wang Y Y Lu L Ma X C Xue Q K | | 0，，： | 1 |
| 3 | A new stage of the nutrition transition in China显示文摘 | Du Shufa Lu Bing Zhai Fengying Barry M Popkin | | 0，，05： | 1 |
| 4 | The siderophore-interacting protein is involved in iron acquisition and virulence of Riemerella anatipestifer strain CH3显示文摘 | Jing Tu Fengying Lu Shuang Miao Xintao Ni Pan Jiang Hui Yu Linlin Xing Shengqing Yu Chan Ding Qinghai Hu | 2013 | Veterinary Microbiology2013，，： | 1 |
| 5 | Study on the COVID-19 epidemic in China's Mainland between November 2022 and January 2023,with prediction of its tendency显示文摘The prediction system EpiSIX was used to study the COVID-19 epidemic in China's Mainland between November 2022 and January 2023,based on reported data from December 9,2022,to January 30,2023,released by The Chinese Center for Disease Control and Prevention on February 1,2023.Three kinds of reported data were used for model fitting:the daily numbers of positive nucleic acid tests and deaths,and the daily number of hospital beds taken by COVID-19 patients.It was estimated that the overall infection rate was 87.54%and the overall case fatality rate was 0.078%–0.116%(median 0.100%).Assuming that a new COVID-19 epidemic outbreak would start in March or April of 2023,induced by a slightly more infectious mutant strain,we predicted a possible large rebound between September and October 2023,with a peak demand of between 800,000 and 900,000 inpatient beds.If no such new outbreak was induced by other variants,then the current COVID-19 epidemic course in China's Mainland would remain under control until the end of 2023.However,it is suggested that the necessary medical resources be prepared to manage possible COVID-19 epidemic emergencies in the near future,especially for the period between September and October 2023. | Yao Bai Zhihang Peng Fengying Wei Zhen Jin Jinjie Wang Ximing Xu Xinyan Zhang Jun Xu Zixiong Ren Bulai Lu Zhaojun Wang Jianguo Xu Senzhong Huang | 2023 | Journal of Biosafety and Biosecurity2023，5，1： | 1 |
| 6 | A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy显示文摘With an intensive understanding of the mechanism of immune system,developing a therapeutic tumor vaccine is one of the most perspective strategy of cancer immunotherapy.In this study,we report a facile approach to prepare graphene oxide(GO)-based therapeutic cancer-nanovaccine.The model antigen(ovalbumin,OVA)and adjuvant(CpG ODN),are conjugated with GO-PEI nanosheet through electrostatic interaction.The addition of PEG can improve biocompatibility and prevent nanoparticle aggregation.The prepared GO-based nanovaccine,GO-PEI-OVA-PEG-CpG,exhibits good biocompatibility and low toxicity both in vivo and in vitro.More importantly,it can efficiently induce the maturation of dendritic cells(DCs),the enhancement of antigen cross-presentation ability,and the amplification of cytokine production of immune cells.Impressively,this nanovaccine shows a remarkable therapeutic effect against preestablished B16-OVA-melanoma tumors,which can significantly inhibit tumor growth and prolong the survival time of the OVA-expressed tumor-bearing mice.Moreover,combining GO-PEI-OVA-PEG-CpG with NLG919,an IDO-1(indoleamine-2,3-dioxygenase)inhibitor which can regulate the tumor microenvironment,displays a synergistic therapeutic effect.These findings indicate the GO-PEI-OVA-PEG-CpG nanovaccine actively induces an antigen-specific antitumor immune response and it combined with NLG919 could achieve better therapeutic outcomes. | Liming Zhang Lingfeng Xu Yi Wang Jieyu Liu Guanghong Tan Fengying Huang Nongyue He Zhuoxuan Lu | 2022 | Chinese Chemical Letters2022，33，8： | 1 |
| 7 | The study of selective primary culture and determination of a breast cancer cell line in vitro显示文摘Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank. | Meng Ren Huixia Xu Xiangji Lu Bingping Wang Rina Su Hao Zhang Song Jiang Fengying Gao Yanwei Gao | 2020 | Oncology and Translational Medicine2020，6，2： | 1 |
| 8 | Prediction Research on Ride Comfort of Special Full Trailer used by Precision Equipment显示文摘In order to evaluate a full trailer’s vibration performance,lumped mass model of seven degrees of freedom is set up to predict the ride comfort of whole vehicle.The acceleration history and the transfer function of each measuring point can be got from simulation.The analysis results show that the maximum acceleration RMS value of the vehicle mass center is about 0.3g,which satisfies the requirements of attenuating vibration of precision equipment being transported on particular roads and speeds.So the research provides valuable reference for the optimum selection of the suspension system’s parameters. | Zhou Changfeng Li Fengying Yao Zhenmin Lu Fangqing | 2013 | 仪器仪表学报2013，34，S1： | 0 |
| 9 | Reduced cell invasion may be a characteristic of placental defects in pregnant women of advanced maternal age at single-cell level显示文摘The mechanisms underlying pregnancy complications caused by advanced maternal age(AMA)remain unclear.We analyzed the cellular signature and transcriptomes of human placentas in AMA women to elucidate these mechanisms.Placental tissues from two AMA women and two controls were used for single-cell RNA-sequencing(scRNA-seq).Controls consisted of AMA women who did not experience any pregnancy complications and pregnant women below the age of 35 years without pregnancy complications.Trophoblast cells were obtained from the placentas of another six pregnant women(three AMA women and three controls),and in-vitro transwell assays were conducted to observe the cell invasion ability.Thirty additional samples(from 15 AMA women and 15 controls)were analyzed to verify the specific expression of serine protease inhibitor clade E member 1(SERPINE1).Preliminary study of the role of SERPINE1 in cell invasion was carried out with HTR8-S/Vneo cells.High-quality transcriptomes of 27607 cells were detected.Three types of trophoblast cells were detected,which were further classified into eight subtypes according to differences in gene expression and Gene Ontology(GO)function.We identified 110 differentially expressed genes(DEGs)in trophoblast cells between the AMA and control groups,and the DEGs were enriched in multiple pathways related to cell invasion.In-vitro transwell assays suggested that the invading trophoblast cells in AMA women were reduced.SERPINE1 was specifically expressed in the trophoblast,and its expression was higher in AMA women(P<0.05).Transfection of human SERPINE1(hSERPINE1)into HTR8-S/Vneo trophoblast cells showed fewer invading cells in the hSERPINE1 group.Impaired cell invasion may underlie the increased risk of adverse pregnancy outcomes in AMA women.Abnormal expression of SERPINE1 in extravillous trophoblast(EVT)cells appears to play an important role. | Bin ZHANG Feng ZHANG Fengying LU Jing WANG Wenbai ZHOU Huihui WANG Bin YU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，9： | 0 |
| 10 | 父代应激调控子代肝脏糖异生显示文摘人群流行病学和动物实验的研究均表明,紧张、焦虑等心理应激可导致和(或)促进多个疾病的发生发展,但其对子代的影响,目前尚不清楚。 | Ling Wu Yan Lu Yang Jiao Bin Liu Shangang Li Yao Li Fengying Xing Dongbao Chen Xing Liu Jiejie Zhao Xuelian Xiong Yanyun Gu Jieli Lu 陈学进 李小英 | 2017 | 科学新闻2017，19，4： | 0 |
| 11 | New Metabolic Alterations and A Predictive Marker Pipecolic Acid in Sera for Esophageal Squamous Cell Carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a major histological subtype of esophageal cancer with a poor prognosis.Although several serum metabolomic investigations have been reported,ESCC tumor-associated metabolic alterations and predictive biomarkers in sera have not been defined.Here,we enrolled 34 treatment-naive patients with ESCC and collected their pre-and post-esophagectomy sera together with the sera from 34 healthy volunteers for a metabolomic survey.Our comprehensive analysis identified ESCC tumor-associated metabolic alterations as represented by a panel of 12 serum metabolites.Notably,postoperative abrosia and parenteral nutrition substantially perturbed the serum metabolome.Furthermore,we performed an examination using sera from carcinogen-induced mice at the dysplasia and ESCC stages and identified three ESCC tumor-associated metabolites conserved between mice and humans.Notably,among these metabolites,the level of pipecolic acid was observed to be progressively increased in mouse sera from dysplasia to cancerization,and it could be used to accurately discriminate between mice at the dysplasia stage and healthy control mice.Furthermore,this metabolite is essential for ESCC cells to restrain oxidative stress-induced DNA damage and cell proliferation arrest.Together,this study revealed a panel of 12 ESCC tumor-associated serum metabolites with potential for monitoring therapeutic efficacy and disease relapse,presented evidence for refining parenteral nutrition composition,and highlighted serum pipecolic acid as an attractive biomarker for predicting ESCC tumorigenesis. | Lei Liu Jia Wu Minxin Shi Fengying Wang Haimin Lu Jibing Liu Weiqin Chen Guanzhen Yu Dan Liu Jing Yang Qin Luo Yan Ni Xing Jin Xiaoxia Jin Wen-Lian Chen | 2022 | Genomics, Proteomics & Bioinformatics2022，20，4： | 0 |
| 12 | Study and prediction of the 2022 global monkeypox epidemic显示文摘The World Health Organization(WHO)declared monkeypox as a public health emergency of international concern(PHEIC)on July 23,2022,their highest level of alert.This raised concerns about the management of the global monkeypox outbreak,as well as the scientific analysis and accurate prediction of the future course of the epidemic.This study used EpiSIX(an analysis and prediction system for epidemics based on a general SEIR model)to analyze the monkeypox epidemic and to forecast the major tendencies based on data from the USA CDC(https://www.cdc.gov)and the WHO(https://www.who.int/health-topics/monkeypox).The global outbreak of monkeypox started in the UK on May 2,2022,which marked the beginning of an epidemic wave.As of October 28,2022,the cumulative number of reported cases worldwide was 77,115,with 36 deaths.EpiSIX simulations predict that the global monkeypox epidemic will enter a low epidemic status on March 1,2023 with the cumulative number of confirmed cases ranging from 85,000 to 124,000,and the total number of deaths ranging from 60 to 87.Our analysis revealed that the basic reproduction number(R0)of monkeypox virus(MPXV)is near to 3.1 and the percentage of asymptomatic individuals is 13.1%–14.5%,both of which are similar to the data for SARS.The vaccination efficiency against susceptibility(VEs)of individuals who have had monkeypox is~79%,and the vaccination efficiency against infectiousness(VEi)of individuals who have had monkeypox is~76%–82%.The mean incubation period for monkeypox is 8 days.In total,94.7%of infected individuals develop symptoms within 20 days and recover within 2 weeks after the confirmation of symptoms.Simulation results using EpiSIX showed that ring vaccination was remarkably effective against monkeypox.Our findings confirmed that a 20-day isolation for close contacts is necessary. | Fengying Wei Zhihang Peng Zhen Jin Jinjie Wang Ximing Xu Xinyan Zhang Jun Xu Zixiong Ren Yao Bai Xiaoli Wang Bulai Lu Zhaojun Wang Jianguo Xu Senzhong Huang | 2022 | Journal of Biosafety and Biosecurity2022，4，2： | 0 |
| 13 | 氯喹衍生物CQ11逆转乳腺癌多药耐药细胞株MCF/DOX对多柔比星的耐药性(英文)显示文摘Objective: To investigate the reversal effect of CQ11, a chloroquine derivative, on multidrug resistance (MDR) in doxorubicin (DOX)-resistant human breast carcinoma cell line MCF/DOX. Methods: Cells of a human breast cancer cell line, MCF, and its DOX-resistant variant, MCF/DOX, were cultivated with DOX and /or CQ11. The cytotoxicity of drugs in vitro was assayed by MTT method. The accumulation of DOX in these cells was detected by fluorescence spectrophotometer. Results: MCF/DOX cells were 119 times more resistant to DOX in comparison with MCF cells. After simultaneous treatment with CQ11 at the concentrations of 1.0, 2.5 and 5.0 μmol/L, the IC50 of DOX for MCF/DOX cells decreased from 3.1 ± 0.47 μmol/L to 0.58 ± 0.032, 0.19 ± 0.012 and 0.081 ± 0.015 μmol/L, respectively, thus, increasing the DOX sensitivity by 5.3-fold (P < 0.01), 16- fold (P < 0.01) and 38-fold (P < 0.01), respectively. In the accumulation assay of DOX, simultaneous incubation of MCF/DOX cells with CQ11 significantly increased the DOX accumulation in MCF/DOX cells. No such results were found in parental MCF cells. Conclusion: CQ11 had strong MDR reversal effect by enhancing intracellular DOX accumulation in MCF/DOX cells, indicating that CQ11 may be a promising MDR chemosensitivity. | Dalong Wu Shirong Ma Fengying Sui Chengwen Zhang Lixin Yin Huanzhang Lu | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，11： | 0 |
| 14 | COVID-19 transmission driven by age-group mathematical model in Shijiazhuang City of China显示文摘Background:A COVID-19 outbreak in the rural areas of Shijiazhuang City was attributed to the complex interactions among vaccination,host,and non-pharmaceutical interventions(NPIs).Herein,we investigated the epidemiological characteristics of all reported symptomatic cases by picking Shijiazhuang City,Hebei Province in Northern China as research objective.In addition,we established an age-group mathematical model to perform the optimal fitting and to investigate the dynamical profiles under three scenarios.Methods:All reported symptomatic cases of Shijiazhuang epidemic(January 2-February 3,2021)were investigated in our study.The cases were classified by gender,age group and location,the distributions were analyzed by epidemiological characteristics.Furthermore,the reported data from Health Commission of Hebei Province was also analyzed by using an age-group mathematical model by two phases and three scenarios.Results:Shijiazhuang epidemic caused by SARS-CoV-2 wild strain was recorded with the peak 84 cases out of 868 reported symptomatic cases on January 11,2021,which was implemented with strong NPIs by local government and referred as baseline situation in this study.The research results showed that R0 under baseline situation ranged from 4.47 to 7.72,and Rt of Gaocheng Distinct took 3.72 with 95%confidence interval from 3.23 to 4.35 on January 9,the declining tendencies of Rt under baseline situation were kept till February 3,the value of Rt reached below 1 on January 19 and remained low value up to February 3 for Gaocheng District and Shijiazhuang City during Shijiazhuang epidemic.This indicated Shijiazhuang epidemic was under control on January 19.However,if the strong NPIs were kept,but remote isolation operated on January 11 was not implemented as of February 9,then the scale of Shijiazhuang epidemic reached 9,482 cases from age group who were 60 years old and over out of 31,017 symptomatic cases.The investigation also revealed that Shijiazhuang epidemic reached 132,648 symptomatic cases for age group who were 60 years old and over(short for G2)under risk-based strategies(Scenario A),58,048 symptomatic cases for G2 under late quarantine strategies(Scenario B)and 207,124 symptomatic cases for G2 under late quarantine double risk strategies(Scenario C),and that the corresponding transmission tendencies of Rt for three scenarios were consistently controlled on Jan 29,2021.Compared with baseline situation,the dates for controlling Rt below 1 under three scenarios were delayed 10 days.Conclusions:Shijiazhuang epidemic was the first COVID-19 outbreak in the rural areas in Hebei Province of Northern China.The targeted interventions adopted in early 2021 were effective to halt the transmission due to the implementation of a strict and village-wide closure.However we found that age group profile and NPIs played critical rules to successfully contain Shijiazhuang epidemic,which should be considered by public health policies in rural areas of China's Mainland during the dynamic zero-COVID policy. | Fengying Wei Ruiyang Zhou Zhen Jin Senzhong Huang Zhihang Peng Jinjie Wang Ximing Xu Xinyan Zhang Jun Xu Yao Bai Xiaoli Wang Bulai Lu Zhaojun Wang Jianguo Xu | 2023 | Infectious Disease Modelling2023，8，4： | 0 |
| 15 | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles显示文摘Background:We aimed to prepare a non-invasive,reproducible,and controllable rat model of intracerebral hemorrhage with focused ultrasound(FUS).Methods:A rat intracerebral hemorrhage(ICH)model was established by combining FUS and microbubbles(μBs),and edaravone was used to verify whether the free radical scavenger had a protective effect on the model.The brain tissue of each group was sectioned to observe the gross histology,blood-brain barrier(BBB)permeability,cerebral infarction volume,and histopathological changes.Results:Compared with the FUS group,the BBB permeability was significantly increased in the FUS+μBs(F&B)group(p=0.0021).The second coronal slice in the F&B group had an obvious hemorrhage lesion,and the FUS+μBs+edaravone(F&B&E)group had smaller hemorrhage areas;however,ICH did not occur in the FUS group.The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group(p=0.0030)and F&B&E group(p=0.0208).HE staining results showed that nerve fibrinolysis,neuronal necrosis,microglia production,and erythrocytes were found in both the F&B group and the F&B&E group,but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group.Conclusions:A rat ICH model was successfully prepared using theμBs assisted FUS treatment,and edaravone had a therapeutic effect on this model.This model can be used to study the pathophysiological mechanism of ICH-related diseases and in preclinical research on related new drugs. | Yao He Jie Yang Fengying Hu Min Liao Yuru Nie Xiaoxia Zhu Tao Zhang Keer Song Qinxi Li Xiaojie Li Chenghan Mei Zhe Wu Qiang Lu Zhihui Zhong | 2023 | Animal Models and Experimental Medicine2023，6，2： | 0 |
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