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| 1 | Quality of life, work productivity impairment and healthcare resources in inflammatory bowel diseases in Brazil显示文摘BACKGROUND Inflammatory bowel diseases(IBD)have been associated with a low quality of life(QoL)and a negative impact on work productivity compared to the general population.Information about disease control,patient-reported outcomes(PROs),treatment patterns and use of healthcare resources is relevant to optimizing IBD management.AIM To describe QoL and work productivity and activity impairment(WPAI),treatment patterns and use of healthcare resources among IBD patients in Brazil.METHODS A multicenter cross-sectional study included adult outpatients who were previously diagnosed with moderate to severe Crohn’s disease(CD)or ulcerative colitis(UC).At enrolment,active CD and UC were defined as having a Harvey Bradshaw Index≥8 or a CD Activity Index≥220 or calprotectin>200μg/g or previous colonoscopy results suggestive of inadequate control(per investigator criteria)and a 9-point partial Mayo score≥5,respectively.The PRO assessment included the QoL questionnaires SF-36 and EQ-5D-5L,the Inflammatory Bowel Disease Questionnaire(IBDQ),and the WPAI questionnaire.Information about healthcare resources and treatment during the previous 3 years was collected from medical records.Chi-square,Fisher’s exact and Student’s t-/Mann-Whitney U tests were used to compare PROs,treatment patterns and the use of healthcare resources by disease activity(α=0.05).RESULTS Of the 407 patients in this study(CD/UC:64.9%/35.1%,mean age 42.9/45.9 years,54.2%/56.6%female,38.3%/37.1%employed),44.7%/25.2%presented moderate-to-severe CD/UC activity,respectively,at baseline.Expressed in median values for CD/UC,respectively,the SF-36 physical component was 46.6/44.7 and the mental component was 45.2/44.2,the EQ-visual analog scale score was 80.0/70.0,and the IBDQ overall score was 164.0/165.0.Moderate to severe activity,female gender,being unemployed,a lower educational level and lower income were associated with lower QoL(P<0.05).Median work productivity impairment was 20%and 5%for CD and UC patients,respectively,and activity impairment was 30%,the latter being higher among patients with moderate to severe disease activity compared to patients with mild or no disease activity(75.0%vs 10.0%,P<0.001).For CD/UC patients,respectively,25.4%/2.8%had at least one surgery,38.3%/19.6%were hospitalized,and 70.7%/77.6%changed IBD treatment at least once during the last 3 years.The most common treatments at baseline were biologics(75.3%)and immunosuppressants(70.9%)for CD patients and 5-ASA compounds(77.5%)for UC patients.CONCLUSION Moderate to severe IBD activity,especially among CD patients,is associated with a substantial impact on QoL,work productivity impairment and an increased number of IBD surgeries and hospitalizations in Brazil. | Rogerio Serafim Parra Julio MF Chebli Heda MBS Amarante Cristina Flores Jose ML Parente Odery Ramos Milene Fernandes Jose JR Rocha Marley R Feitosa Omar Feres Antonio S Scotton Rodrigo B Nones Murilo M Lima Cyrla Zaltman Carolina D Goncalves Isabella M Guimaraes Genoile O Santana Ligia Y Sassaki Rogerio S Hossne Mauro Bafutto Roberto LK Junior Mikaell AG Faria Sender J Miszputen Tarcia NF Gomes Wilson R Catapani Anderson A Faria Stella CS Souza Rosana F Caratin Juliana T Senra Maria LA Ferrari | 2019 | World Journal of Gastroenterology2019，25，38： | 2 |
| 2 | Ethidium bro- mide-induced demyelination of the sciatic nerve of adult Wistar rats显示文摘 | Riet-Correa G Fernandes CG Pereira LA | 2002 | Braz J Med Biol Res2002，35，1： | 1 |
| 3 | The nature of focal areas of hyperfluorescence or 'hot spots' imaged with indocyanine green angiography显示文摘 | Fernandes LH Freund KB Yannuzzi LA Spaide RF Huang SJ Slakter JS | 2002 | Retina2002，22，： | 1 |
| 4 | New macrolides active against Streptococcus pyogenes with inducible or constitutive type of macrolide-lincosamide-streplogramin B resistance显示文摘 | Baker WR Freiberg LA | 1989 | Antimacrob Agents Chemother1989，33，1： | 1 |
| 5 | Protein disulfide isomerase in redox cell signaling and homeostasis 显示文摘 | Laurindo FR Pescatore LA Fernandes DC | 2012 | Free Radic Biol Meal2012，52，： | 1 |
| 6 | Effeets of age on elements of insulin signaling pathway in central nervous system of rats显示文摘 | Fernandes ML Saad MJ Velloso LA | 2001 | Endocrine2001，16，3： | 1 |
| 7 | Effects of age on elements of insulin-signaling pathway in cerebral nervous system of rats显示文摘 | Fernandes ML Saad MJ Velloso LA | | 0，，3： | 1 |
| 8 | Stromal cells playa role in cervical cancer progression mediated by MMP-2 protein显示文摘 | Fernandes T de Angelo-Andrade LA Morais SS | 2008 | Eur J Gynaecol Onco2008，29，4： | 1 |
| 9 | Assessment of the effectiveness of pharmacotherapy follow-up in patients treated for depression显示文摘 | Marques LA Galdur6z JC Fernandes MR | | 0，，03： | 1 |
| 10 | Frequency of amoebiasis and other intestinal parasitoses in a settlement in Ilh 6 us City, State of Bahia, Brazil显示文摘 | Carneiro Santos HL Fernandes Martins LA Saramago Peralta RH | 2014 | Rev Soc Bras Med Trop2014，47，1： | 1 |
| 11 | Effects of age on elements of insulinsignaling pathway in central nervous system of rats显示文摘 | Fernandes ML Saad MJ Velloso LA | 2001 | Endocrine2001，16，： | 1 |
| 12 | Demyelination and remyeli- nation after multiple intramedullary injections of ethidium bromide in Wistar rats 显示文摘 | Fernandes CG Graca DL Pereira LA | 1997 | Arq Neuropsiquiatr1997，55，： | 1 |
| 13 | Nonsurgieal Periodontal Therapy Combined with Laser and Photodynamic Therapies for Periodontal Disease in Immunosuppressed Rats 显示文摘 | Bottura PE Milanezi J Fernandes LA | 2011 | Elsevier2011，43，5： | 1 |
| 14 | Stromalcells play a role in cervical cancer progression mediated by MMP-2 protein显示文摘 | Fernandes T de Angelo-Andrade LA Morais SS | 2008 | Eur J Gynaecol Oncol2008，29，4： | 1 |
| 15 | The nature of focal areas of hyperfluorescence or hot spots imaged with indocyanine green angiography 显示文摘 | Fernandes LH Freund KB Yannuzzi LA | 2002 | Retina2002，22，： | 1 |
| 16 | Stromal cells play a role in cervical cancer progression mediated by MMP- 2 protein 显示文摘 | Fernandes T de Angelo-Andrade LA Morais SS | 2008 | Eur J Gynaecol Oncol2008，29，4： | 1 |
| 17 | Comparison be- tween laser therapy and non-surgical therapy for periodontitis in rats treated with dexamethasone 显示文摘 | Garcia VG Fernandes LA de Almeida JM | 2010 | Laser Med Sci2010，25，2： | 1 |
| 18 | Treatment of experi- mental periodontal disease with antimicrobial photodynamic therapy in nicotine-modified rats 显示文摘 | Garcia VG Fernandes LA Maearini VC | 2011 | J Clin Periodontal2011，38，12： | 1 |
| 19 | Comparison between laser therapy and non-surgical therapy for periodontitis in rats treated with dexamethasone显示文摘 | Garcia VG Fernandes LA de AlmeidaJM | 2010 | Lasers Med Sci2010，25，2： | 1 |
| 20 | In vitro induced pluripotency from urine-derived cells in porcine显示文摘BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible protocols.The porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal production.AIM To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine urine.METHODS The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)supplementation.To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSEA 1 detection.Endogenous transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF supplementation.RESULTS The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming.Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent state.All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts.CONCLUSION For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotentlike state,enabling new numerous applications in both human or veterinary regenerative medicine. | Kaiana Recchia Lucas Simões Machado Ramon Cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de Castro Mariana Groke Marques Laís Vicari de Figueiredo Pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline Fernanda de Souza Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan | 2022 | World Journal of Stem Cells2022，14，3： | 0 |
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