|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | A prospective feasibility study of sub-millisievert abdominopelvic CT using iterative reconstruction in Crohn’s disease显示文摘 | Siobhan B O’Neill Patrick D Mc Laughlin Lee Crush Owen J O’Connor Sebastian R Mc Williams Orla Craig Anne Marie Mc Garrigle Fiona O’Neill Jackie Bye Max F. Ryan Fergus Shanahan Michael M Maher | 2013 | European Radiology2013，，： | 2 |
| 3 | A Risk Register Database System to Aid the Management of Project Risk 显示文摘 | Fiona D P Kevin N | 2002 | International Journal of Project Management2002，20，5： | 1 |
| 4 | Gait classification in children with cerebral palsy: A systematic review显示文摘 | Fiona D Morris ME Baker R | 2007 | Gait & Posture2007，25，1： | 1 |
| 5 | Novel approaches to detect serum biomarkers for clinical response to interferon - β treatment in multiple sclerosis显示文摘 | Kaushal S Fiona C Eve D | 2010 | Plos One2010，5，10： | 1 |
| 6 | The application of settling velocity as a parameter for characterising wastewater solids显示文摘 | Hedges Peter D Becker Fiona A Smisson Robert PM | 1998 | Wat Sci Tech1998，37，1： | 1 |
| 7 | Gas chromatogra-mphic mass spectrometric determination of β 2-agonists in postmortem blood:application in forensic medicine显示文摘 | Couper B R Fiona J Drummer A D | 1996 | Chromatography Biomedical Science and Application1996，685，2： | 1 |
| 8 | A risk register database system to aid the management of project risk 显示文摘 | Fiona D Patterson Kevin Neailey | 2002 | International Journal of Project Management2002，20，5： | 1 |
| 9 | Mycoplasmapneumoniae infection in a podiatric population: Analysis of soluble immune markers as risk factors for asthma显示文摘 | Jaythoon H Fiona I Soamus D | 2008 | Hum Immunol2008，69，12： | 1 |
| 10 | Subtotal hepatectomy: a porcine model for the study of liver regeneration显示文摘 | Fiona G Court Peter E Laws Charles P Morrison Benjamin D Teague Matthew S Metcalfe Simon A Wemyss-Holden Ashley R Dennison Guy J Maddern | 2004 | Journal of Surgical Research2004，，1： | 1 |
| 11 | Effects of thalidomide and related metabolites in a mouse corneal model of neovascularization显示文摘 | Kenyon BM Fiona Browne D'amato RJ | 1997 | Exp Eye Res1997，64，6： | 1 |
| 12 | Abdominal muscle function in chronic low back pain patients: measurement with real-time ultrasound scanning显示文摘 | Critchley D J Fiona JC | 2002 | Physiotherapy2002，88，： | 1 |
| 13 | The application of settling velocity as a parameter for characterising wastewater solids 显示文摘 | Hedges Peter D Becker Fiona A Smisson Robert P M | 1998 | Wat Sci Tech1998，37，1： | 1 |
| 14 | Early and midterm outcome after off-pump and on-pump surgery in Beating Heart Against Cardioplegic Arrest Studies (BHACAS 1 and 2): a pooled analysis of two randomised controlled trials显示文摘 | Gianni D Angelini Fiona C Taylor Barnaby C Reeves Raimondo Ascione | 2002 | The Lancet . 2002 (9313)2002，，9313： | 1 |
| 15 | A thermodynamic approach to ion flotation (II) Metal ion selectivity in the SDS-Cu- Ca and SDS- Cu - Pb systems 显示文摘 | Liu Zhendong Fiona M D | 2000 | Coil and Surf APhysicochem and Eng Asp2000，178，： | 1 |
| 16 | Bioscience in the Pre-Registration Curriculum: Finding the Right Teaching Strategy显示文摘 | Shan D Fiona M Sue J | 2000 | Nurse Education Today2000，20，2： | 1 |
| 17 | Activated sludge foaming: The true extent ofactinomycete diversity显示文摘 | Michael G Fiona M S Russel D | 1998 | Water Res1998，37，45： | 1 |
| 18 | Global burden of disease in young people aged 10–24 years: a systematic analysis显示文摘 | Fiona M Gore Paul JN Bloem George C Patton Jane Ferguson Véronique Joseph Carolyn Coffey Susan M Sawyer Colin D Mathers | 2011 | 2011 (9783)2011，，9783： | 1 |
| 19 | Wave-driven zonal flow vacillation in the Southern Hemisphere 显示文摘 | Hartmann D L Fiona L | 1998 | Journal of the Atmospheric Sciences1998，55，： | 1 |
| 20 | Global burden of disease in young people aged 10–24 years: a systematic analysis显示文摘 | Fiona M Gore Paul JN Bloem George C Patton Jane Ferguson Véronique Joseph Carolyn Coffey Susan M Sawyer Colin D Mathers | 2011 | The Lancet . 2011 (9783)2011，，9783： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员