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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Microsurgical clipping of pre-viously coiled intraeranial aneurysms 显示文摘 | Nakamura M Montibeller G R G tz F | 2013 | Clinieal neurology and neuro-surgery2013，115，8： | 1 |
| 3 | Anabolic effect of intermittent PTH(1-34) on the local microenvironment during the late phase ofperiodontal repair in a rat model of tooth root resorption显示文摘 | Lossd rfer S Yildiz F G tz W | | 0，，01： | 1 |
| 4 | Laparoscopic appendectomy显示文摘 | Pier A Gtz F Bacher C | 1993 | World J Surg1993，17，1： | 1 |
| 5 | Amyloid-induced neurofibrillary tangle formation in Alzheimer' s disease:insight from transgenic mouse and tissue-culture models显示文摘 | G(o)tz J Schild A Hoerndli F | | 0，，07： | 1 |
| 6 | MITOS:Improved de novo metazoan mitochondrial genome annotation显示文摘 | Bernt M Donath A Jühling F Externbrink F Florentz C Fritzsch G Pütz J Middendorf M Stadler PF | | 0，，： | 1 |
| 7 | Modified laparoscopic appendectomy in surgery:a report on 388 operations显示文摘 | G tz F Pier A Bacher C | 1990 | Surg Endosc1990，4，1： | 1 |
| 8 | Oligomeric and fibrillar species of β-amyloid(Aβ42) both impair mitochondrial function in P301L tau transgenic mice显示文摘We recently provided evidence for a mitochondrial dysfunction in P301L tau transgenic mice,a strain modeling the tau pathology of Alzheimer's disease(AD)and frontotemporal dementia(FTD).In addition to tau aggregates,the AD brain is further characterized by Aβpeptide-containing plaques.When we addressed the role of Aβ,this indicated a synergistic action of tau and Aβpathology on the mitochondria.In the present study,we compared the toxicity of different Aβ42 conformations in light of recent studies suggesting that oligomeric rather than fibrillar Aβmight be the actual toxic species.Interestingly,both oligomeric and fibrillar,but not disaggregated(mainly monomeric)Aβ42 caused a decreased mitochondrial membrane potential in cortical brain cells obtained from FTD P301L tau transgenic mice.This was not observed with cerebellar preparations indicating selective vulnerability of cortical neurons.Furthermore,we found reductions in state 3 respiration,the respiratory control ratio,and uncoupled respiration when incubating P301L tau mitochondria either with oligomeric or fibrillar preparations of Aβ42.Finally,we found that aging specifically increased the sensitivity of mitochondria to oligomeric Aβ42 damage indicating that oligomeric and fibrillar Aβ42 are both toxic,but exert different degrees of toxicity. | Anne Eckert Susanne Hauptmann Isabel Scherping Jessica Meinhardt Virginie Rhein Stefan Drse Ulrich Brandt Marcus Fndrich Walter E.Müller Jiirgen Gtz | 2016 | 世界最新医学信息文摘2016，16，2： | 1 |
| 9 | Microsurgical clipping of previously coiled intracranial aneurysms显示文摘 | Nakamura M Montibeller GR G(o)tz F | 2013 | Clin Neurol Neurosurg2013，115，8： | 1 |
| 10 | Anabolic effect of intermittent PTH(1 -34) on the local microenvironment during the late phase of periodontalrepair in a rat model of tooth root resorption显示文摘 | Lossd(o)rfer S Yildiz F G(o)tz W | | 0，，01： | 1 |
| 11 | Tau filament formation in transgenic mice expressing P301L tau显示文摘 | G?tz J Chen F Barmettler R | 2001 | J Biol Chem2001，276，1： | 1 |
| 12 | Polybrominated Diphenyl Ethers Induce Developmental Neurotoxicity in a Human in Vitro Model: Evidence for Endocrine Disruption显示文摘 | Schreiber Timm Gassmann Kathrin G?tz Christine Hübenthal Ulrike Moors Michaela Krause Guido Merk Hans F Nguyen Ngoc-Ha Scanlan Thomas S Abel Josef Rose Christine R Fritsche Ellen | 2010 | Environmental Health Perspectives2010，，4： | 1 |
| 13 | Modeling of scale formation and corrosion from geothermal water显示文摘 | P(a)tz(a)y G St(a)hl G K(a)rm(a)n F H | 1998 | Electrochimica Acta1998，43，12： | 1 |
| 14 | The archaeogenomic validation of Saint Ladislaus'relic provides insights into theárpád dynasty's genealogy显示文摘The house of Arp ad ruled Hungary for more than four centuries,establishing dynastical relationships with numerous European nobleand ruling houses,and giving many Catholic saints and blesseds to the Catholic Church.They were one of the significant dynasties of Medieval Europe(Krist o and Makk,1996).The knight-king Saint Ladislaus I(c.1040-1095,reign:1077-1095)is one of the most outstanding kings of this dynasty.In addition to the hand relic(the Holy Right)of King Saint Stephanus I(c.975-1038,reign:1000/1001-1038),the skull relic in the Saint Ladislaus’Herma(Fig.1A-1C)preserved in the Cathedral of Gy or,is one of the most important relics for Hungarians(Klaniczai,2000). | Gergely I.B.Varga Lilla Alida Kristóf Kitti Maár Luca Kis Oszkár Schütz Orsolya Váradi Bence Kovács Alexandra Gînguță Balázs Tihanyi Péter L.Nagy Zoltán Maróti Emil Nyerki Tibor Török Endre Neparáczki | 2023 | Journal of Genetics and Genomics2023，50，1： | 0 |
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