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| 1 | Sorafenib inhibits growth and metastasis of hepatocellular carcinoma by blocking STAT3显示文摘AIM: To investigate the inhibitory role and the underlying mechanisms of sorafenib on signal transducer and activator of transcription 3 (STAT3) activity in hepatocellular carcinoma (HCC).METHODS: Human and rat HCC cell lines were treated with sorafenib. Proliferation and STAT3 dephosphorylation were assessed. Potential molecular mechanisms of STAT3 pathway inhibition by sorafenib were evaluated. In vivo antitumor action and STAT3 inhibition were investigated in an immunocompetent orthotopic rat HCC model.RESULTS: Sorafenib decreased STAT3 phosphorylationat the tyrosine and serine residues (Y705 and S727), but did not affect Janus kinase 2 (JAK2) and phosphatase shatterproof 2 (SHP2), which is associated with growth inhibition in HCC cells. Dephosphorylation of S727 was associated with attenuated extracellular signal-regulated kinase (ERK) phosphorylation, similar to the effects of a mitogen-activated protein kinase (MEK) inhibitor U0126, suggesting that sorafenib induced S727 dephosphorylation by inhibiting MEK/ERK signaling. Meanwhile, sorafenib could also inhibit Akt phosphorylation, and both the phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 and Akt knockdown resulted in Y705 dephosphorylation, indicating that Y705 dephosphorylation by sorafenib was mediated by inhibiting the PI3K/Akt pathway. Finally, in the rat HCC model, sorafenib signifi cantly inhibited STAT3 activity, reducing tumor growth and metastasis.CONCLUSION: Sorafenib inhibits growth and metastasis of HCC in part by blocking the MEK/ERK/STAT3 and PI3K/Akt/STAT3 signaling pathways, but independent of JAK2 and SHP2 activation. | Fang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian ZhouFang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian Zhou, Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical School, Fudan University, Shanghai 200032, China Author contributions: Gu FM, Li QL and Gao Q contributed equally to this work Gu FM and Li QL performed the experi- ments and interpretation of the data and statistical analysis Zhou J and Gao Q contributed to the conception and design of the study Gu FM, Gao Q, Li QL and Zhou J wrote the manuscript Jiang JH, Huang XY, Pan JF and Fan J made substantial contri- bution to the design and conception of the study and interpreta- tion of data all authors read and approved the f inal manuscript. | 2011 | World Journal of Gastroenterology2011，17，34： | 18 |
| 2 | Leaf orientation, photorespiration and xanthophyll cycle protect young soybean leaves against high irradiance in field显示文摘 | Jiang, CD Gao, HY Zou, Q Jiang, GM Li, LH | 2006 | 中国生物学文摘2006，20，2： | 12 |
| 3 | Super-resolution dipole orientation mapping via polarization demodulation显示文摘Fluorescence polarization microscopy(FPM)aims to detect the dipole orientation of fluorophores and to resolve structural information for labeled organelles via wide-field or confocal microscopy.Conventional FPM often suffers from the presence of a large number of molecules within the diffraction-limited volume,with averaged fluorescence polarization collected from a group of dipoles with different orientations.Here,we apply sparse deconvolution and least-squares estimation to fluorescence polarization modulation data and demonstrate a super-resolution dipole orientation mapping(SDOM)method that resolves the effective dipole orientation from a much smaller number of fluorescent molecules within a sub-diffraction focal area.We further apply this method to resolve structural details in both fixed and live cells.For the first time,we show that different borders of a dendritic spine neck exhibit a heterogeneous distribution of dipole orientation.Furthermore,we illustrate that the dipole is always perpendicular to the direction of actin filaments in mammalian kidney cells and radially distributed in the hourglass structure of the septin protein under specific labelling.The accuracy of the dipole orientation can be further mapped using the orientation uniform factor,which shows the superiority of SDOM compared with its wide-field counterpart as the number of molecules is decreased within the smaller focal area.Using the inherent feature of the orientation dipole,the SDOM technique,with its fast imaging speed(at sub-second scale),can be applied to a broad range of fluorescently labeled biological systems to simultaneously resolve the valuable dipole orientation information with super-resolution imaging. | Karl Zhanghao Long Chen Xu-San Yang Miao-Yan Wang Zhen-Li Jing Hong-Bin Han Michael Q Zhang Dayong Jin Jun-Tao Gao Peng Xi | 2016 | Light(Science & Applications)2016，5，1： | 11 |
| 4 | Intrahepatic transplantation of hepatic oval cells for fulminant hepatic failure in rats显示文摘AIM:To evaluate the effect of intrahepatic transplantation of hepatic oval cells(HOC)on fulminant hepatic failure(FHF)in rats.METHODS:HOC obtained from rats were labeled with green fluocescent protein(GFP)or 5,6carboxyfluorescein diacetate succinmidyl ester(CFDASE).Cell fluorescence was observed under fluorescent microscope at 6,24,48 and 72 h after labeling.CFDASE labeled HOC(5×10 6 cells each rat)were injected into livers of rats with FHF induced by D-galactosamine.Serum albumin(ALB),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBil)levels were measured at different time points.Liver function of rats was examined on days 3,7,14 and 21 after HOC transplantation.RESULTS:The positive rate of GFP and CFDA-SE labeled HOC was 10%and 90%,respectively,with no significant change in cell viabilities.The survival rate was higher in HOC transplantation group than in control group,especially 48(9/15 vs 6/15)and 72 h(9/15 vs 4/15)after HOC transplantation.The serum ALT,AST and TBil levels were decreased while the serum Alb level was increased after HOC transplantation.Fluorescence became faded and diffused in liver tissues,suggesting that proliferation and differentiation occur in transplanted HOC.CONCLUSION:CFDA-SE is superior to GFP in labeling HOC,although fluorescence intensity is decreased progressively with cell division.HOC transplantation can improve the liver function and increase the survival rate of recipients. | Chen-Xuan Wu,Qi Zou,Zheng-Yan Zhu,Ying-Tang Gao,Yi-Jun Wang,Department of Hepatobiliary,The Third Center of Affiliated Hospital of Tianjin Medical University Key Laboratory of Artificial Cells of Tianjin,Tianjin 300170,China Author contributions:Wang YJ,Wu CH and Zou Q designed the research Zou Q,Zhu ZY,Gao YT performed the research Zou Q analyzed the data Wang YJ and Wu CX wrote the paper.Supported by Tianjin Science Committee,Grant No.05SYSYJC02600 and Tianjin Health Bureau,Grant No.05KYR01 | 2009 | World Journal of Gastroenterology2009，15，12： | 7 |
| 5 | 脂蛋白a水平与冠状动脉粥样硬化性心脏病患者复发事件的关系显示文摘脂蛋白a是否是冠状动脉粥样硬化性心脏病(冠心病)患者复发性心血管事件(recurrent cardiovascular events,RCVEs)的预测因子尚未确定。本研究旨在探讨脂蛋白a对一个真实世界、大规模首诊心血管事件(cardiovascular event,CVE)患者队列中RCVEs的潜在影响。方法:本实验为多中心的前瞻性研究,纳入经血管造影诊断的冠心病并经历首次CVE的患者7562例。入院时测量所有患者的脂蛋白a浓度. | 周卫(译) 叶鹏(校) Liu HH Cao YX Jin JL Zhang HW Hua Q Li YF Guo YL Zhu CG Wu NQ Gao Y Xu RX Hong LF | 2020 | 中华高血压杂志2020，28，5： | 5 |
| 6 | 在体背根神经节钙成像方法解析内脏痛及牵涉痛的新机制显示文摘内脏痛是指来自机体内在器官病理改变或功能异常引起的疼痛,常与多种内脏疾病相关,如急慢性肠炎等。内脏痛往往伴有躯体一定部位的牵涉痛。到目前为止,内脏痛及其牵涉痛的机制仍不清楚。该研究采用在体背根神经节钙成像方法(in vivo DRG calcium imaging),大视野实时动态观察和研究内脏痛及牵涉痛的背根节(dorsal root ganglion,DRG)神经元相关机制。 | 刘风雨(译) Gao XY Han S Huang Q | 2021 | 中国疼痛医学杂志2021，27，10： | 5 |
| 7 | Application of metamaterials to ultra-thin radar-absorbing material design显示文摘 | Gao Q Yin Y Yan D B | | 0，，17： | 2 |
| 8 | Enhanced photoprotection at the early stages of leaf expansion in fieldgrown soybean plants显示文摘 | Jiang, CD Li, PM Gao, HY Zou, Q Jiang, GM Li, LH | 2006 | 中国生物学文摘2006，20，2： | 2 |
| 9 | 妊娠期基因-环境相互作用与母体砷甲基化效率显示文摘单核昔酸多态性(SNPs)主要是指在基因组水平上由单个核昔酸的变异所引起的DNA序列多态性,是人类可遗传的变异中最常见的一种,占所有已知多态性的80%以上。有研究表明,SNPs可能通过影响砷的甲基化效干扰砷代谢。而环境与基因的交互作用是否会影响孕期砷代谢目前尚不明确,因此,本研究分析SNP-砷的交互作用对砷甲基化效率的影响。 | Gao S Mostofa MG Quamruzzaman Q 李园园(编译) | 2019 | 中华预防医学杂志2019，53，5： | 2 |
| 10 | Generation of continuous and pulsed diagnostic imaging X-ray radiation using a carbonnanotube based field-emission cathode显示文摘 | YUE G Z QIU Q GAO B | 2002 | Appl Phys Lett2002，181，2： | 2 |
| 11 | Effects of direct intravitreal dopamine injections on the development of lid-suture induced myopia in rabbits 显示文摘 | Gao Q Liu Q Ma P | 2006 | Graefes Arch Clin Exp Ophthalmol2006，44，10： | 1 |
| 12 | Prevention of Thl responses is critical for tolerance显示文摘 | Chen N gao Q Field EH | 1996 | Transplantation1996，61，7： | 1 |
| 13 | Studies on the micropolarities of /TX-100/toluene ionic liquid microemulsions andtheir behaviors characterized by UV - visible spectroscopy 显示文摘 | Li N Gao Y A Zheng L Q | 2007 | Langmuir2007，23，： | 1 |
| 14 | 显示文摘 | Sohlberg K Xue Z Q | 2000 | Phys Rev Lett2000，84，： | 1 |
| 15 | 显示文摘 | Gao D S Chen W Xu Q | 2000 | J Wuhan Univ Techol Mater Sci Ed2000，15，： | 1 |
| 16 | Detection of pl6 hypermethylation in circulating plasma DNA of non-small cell lung cancer patients显示文摘 | An Q Liu Y Gao Y | 2002 | Cancer Lett2002，188，12： | 1 |
| 17 | Enhanced adhesion of diamond-like carbon films with a compositional-graded intermediate layer显示文摘 | Hou Q R Gao J | 1999 | Applied Physics A:Materials Science & Processing1999，680，： | 1 |
| 18 | Role of hypoxia in obesity-induced disorders of glucose and lipid metabolism in adipose,tissue显示文摘 | Yin J Gao Z He Q | 2009 | Am J Physiol Endocrinol Metab2009，296，2： | 1 |
| 19 | The effects of food and the sum of effective temperature on the embryonic development of the seahorse,Hippocampus kuda Bleeker 显示文摘 | LIN Q GAO Y SHENG J | 2007 | Aquacuhure2007，262，24： | 1 |
| 20 | An unmediated hydrogen peroxide biosensor based on hemoglobin incorporated in a montmorillonite membrane 显示文摘 | FAN C GAO Q ZHU D | 2001 | The Analyst2001，126，7： | 1 |
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