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| 1 | Dextran sulfate sodium-induced acute colitis impairs dermal lymphatic function in mice显示文摘AIM: To investigate whether dermal lymphatic function and architecture are systemically altered in dextran sulfate sodium(DSS)-induced acute colitis.METHODS: Balb/c mice were administered 4% DSS in lieu of drinking water ad libitum for 7 d and monitored to assess disease activity including body weight, diarrhea severity, and fecal bleeding. Control mice received standard drinking water with no DSS. Changes in mesenteric lymphatics were assessed following oral administration of a fluorescently-labelled fatty acid analogue, while dermal lymphatic function and architecture was longitudinally characterized using dynamic near-infrared fluorescence(NIRF) imaging following intradermal injection of indocyanine green(ICG) at the base of the tail or to the dorsal aspect of the left paw prior to, 4, and 7 d after DSSadministration. We also measured dye clearance rate after injection of Alexa680-bovine serum albumin(BSA). NIRF imaging data was analyzed to reveal lymphatic contractile activity after selecting fixed regions of interest(ROIs) of the same size in fluorescent lymphatic vessels on fluorescence images. The averaged fluorescence intensity within the ROI of each fluorescence image was plotted as a function of imaging time and the lymphatic contraction frequency was computed by assessing the number of fluorescent pulses arriving at a ROI. RESULTS: Mice treated with DSS developed acute inflammation with clinical symptoms of loss of body weight, loose feces/watery diarrhea, and fecal blood, all of which were aggravated as disease progressed to 7 d. Histological examination of colons of DSS-treated mice confirmed acute inflammation, characterized by segmental to complete loss of colonic mucosa with an associated chronic inflammatory cell infiltrate that extended into the deeper layers of the wall of the colon, compared to control mice. In situ intravital imaging revealed that mice with acute colitis showed significantly fewer fluorescent mesenteric lymphatic vessels, indicating impaired uptake of a lipid tracer within mesenteric lymphatics. Our in vivo NIRF imaging data demonstrated dilated dermal lymphatic vessels, which were confirmed by immunohistochemical staining of lymphatic vessels, and significantly reduced lymphatic contractile function in the skin of mice with DSS-induced acute colitis. Quantification of the fluorescent intensity remaining in the depot as a function of time showed that there was significantly higher Alexa680-BSA fluorescence in mice with DSSinduced acute colitis compared to pre-treatment with DSS, indicative of impaired lymphatic drainage.CONCLUSION: The lymphatics are locally and systemically altered in acute colitis, and functional NIRF imaging is useful for noninvasively monitoring systemic lymphatic changes during inflammation. | Germaine D Agollah Grace Wu Ho-Lan Peng Sunkuk Kwon | 2015 | World Journal of Gastroenterology2015，21，45： | 2 |
| 2 | Targeting the cytoplasmic and nuclear functions of signal transducers and activators of tran- scription 3 for cancer therapy显示文摘 | GERMAIN D FRANK D A | 2007 | Clin Cancer Res2007，13，19： | 1 |
| 3 | Targeting the cytoplasmic and nuclear functionsof signal transducers and activators of transcription 3 for Cancertherapy显示文摘 | Germain D Frank DA | 2007 | Clin Cancer Res2007，13，19： | 1 |
| 4 | Manage- ment of thecritiea/ly ill obstetric patient显示文摘 | Price L C Germain S Wyncoll D& Nelson - Pierey C | 2009 | Obstetrics Gynaecolo- gy & Reproductive Medicine2009，19，12： | 1 |
| 5 | Feature-based reverse engineering of mechanical parts显示文摘 | Thompson W B Owen J C St Germain H J D | 1999 | IEEE Transactions on Robotics and Automation1999，15，1： | 1 |
| 6 | Solubilization of the neutral and charged forms of 2,4,6-trichlorophenoI by β-cyclodextrin, methyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin in water显示文摘 | HANNA K BRAUER C D GERMAIN P | 2003 | Journal of Hazardous Materials2003，94，2： | 1 |
| 7 | Cyclodextrin-enhanced solubilization of pentachlorophenoI in water显示文摘 | HANNA K BRAUER C D GERMAIN P | 2004 | Journal of Environmental Management2004，71，1： | 1 |
| 8 | Deployment-related insomnia inmilitary personnel and veterans 显示文摘 | Bramoweth A D Germain A | 2013 | Curr Psychiatry Rep2013，15，10： | 1 |
| 9 | NVL: a new member of the AAA family of ATPases localized to the nucleus显示文摘 | Obie C Valle D | 1997 | Genomics1997，15，41： | 1 |
| 10 | Effects of abutment size and luting cement type on the uniaxial reten- tion force of implant-supported crowns显示文摘 | Covey D A Kent D K St Germain Jr H A | 2000 | The Journal of prosthetic dentistry2000，83，3： | 1 |
| 11 | Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid显示文摘 | Germaine K J Liu X Cabellos GG Hogan JP Ryan D Dowling DN | | 0，，02： | 1 |
| 12 | Cystatin C as a marker of early changes of renal function in Fabry nephropathy显示文摘 | Feriozzi S Germain D P Di Vito R | 2007 | J Nephrol2007，20，4： | 1 |
| 13 | Ubiquitin-dependent and independent mitochondrial protein quality controls:implications in ageing and neurodegener- ative diseases显示文摘 | Germain D | 2008 | Mol Microbiol2008，70，6： | 1 |
| 14 | Targeting the cytoplasmic and nuclear functions of signal transducers and activators of transcription 3 for cancertherapy 显示文摘 | Germain D Frank DA | 2007 | Clin Cancer Res2007，13，19： | 1 |
| 15 | Functional neuroimaging evidence for hyperarousal in insomnia 显示文摘 | Nofzinger EA Buysse D J Germain A | 2004 | Am J Psychiatry2004，161，11： | 1 |
| 16 | Estrogen receptor mediates a dis- tinct mitochondrial unforded protein response 显示文摘 | Papa L Germain D | 2011 | Journal of Cell Science2011，124，13： | 1 |
| 17 | SirT3 regulates the mitochondrial unfolded protein response显示文摘 | Papa L Germain D | 2014 | Molecular and Cellular Biology2014，34，4： | 1 |
| 18 | Silver nanodisks:size selection via centrifugation and optical properties显示文摘 | Germain V Brioude A Ingert D | 2005 | Journal of Chemical Physics2005，122，12： | 1 |
| 19 | Differential expression of the F-box proteins Skp2 and Skp2B in breast cancer显示文摘 | Radke S Pirkmaier A Germain D | 2005 | Oncogene2005，24，21： | 1 |
| 20 | Regulation of apoptosis by endoplasmic reticulum pathways显示文摘 | Breckenridge D G Germain M Mathai J P Nguyen M Shore G C | 2003 | Oncogene2003，22，53： | 1 |
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