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| 1 | Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells显示文摘AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after neurotmesis.METHODS: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the 'minimal Feret's diameter' respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible that might prevent the secondary neurogenic muscular atrophy by improving the reestablishment of the neuro-muscular junction. | Jorge Ribeiro Tiago Pereira Ana Rita Caseiro Paulo Armada-da-Silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício | 2015 | World Journal of Stem Cells2015，7，6： | 2 |
| 2 | Science-technology linkages in an emerging research platform: The case of combinatorial chemistry and biology 显示文摘 | Geuna A Malo S | 2000 | SCIENTOMETRICS2000，47，2： | 1 |
| 3 | Long-termfunctional and morphological assessment of a stand-ardized rat sciatic nerve crush injury with a non-ser-rated clamp显示文摘 | Luis AL Amado S Geuna S | 2007 | Neurosci Methods2007，163，1： | 1 |
| 4 | A stereological study of long- term regeneration of rat severed siatic nerve repaired by means of muscle-vein-combined grafts显示文摘 | Geuna S Tos P Battiston B | 2000 | Ital Anat Ernbryol2000，103，： | 1 |
| 5 | Phototherapy and nerve injury:focus on muscle response显示文摘 | Rochkind S Geuna S Shainberg A | 2013 | Int Rev Neurobiol2013，109，： | 1 |
| 6 | Nerve repair by means of tubulization: literature review and personal clinical experience comparing biological and synthetic conduits 显示文摘 | Battiston B Geuna S Ferrero M | 2005 | Mierosurgery2005，25，4： | 1 |
| 7 | The use of brainstorming for teaching human anatomy 显示文摘 | Geuna S Giacobini-Robecchi MG | 2002 | Anat Rec2002，269，5： | 1 |
| 8 | Chapter 3:Histology of the peripheral nerve and changes occurring during nerve regeneration 显示文摘 | Geuna S Raimondo S Ronchi G | 2009 | International review of neurobiology2009，87，： | 1 |
| 9 | Human T lymphocytes transduced by lentiviral vectors in the absence of TCR activation maintain an intact immune competence 显示文摘 | Cavalieri S Cazzaniga S Geuna M | 2003 | Blood2003，102，2： | 1 |
| 10 | Expression of alpha2a-2b neuregulin-1 is associated with early periph-eral nerve repair along muscle-enriched tubes显示文摘 | Nicolino S Raimondo S Tos P Battiston B Fornaro M Geuna S Perroteau I | | 0，，： | 1 |
| 11 | Chapter 25:Phototherapy in peripheral nerve injury:effects on muscle preservation and nerve regeneration显示文摘 | Rochkind S Geuna S Shainberg A | | 0，，： | 1 |
| 12 | Unscheduled DNA synthesis in rat adult myenteric neurons: an immunohistochemical study 显示文摘 | Corvetti G Fornaro M Geuna S | 2001 | Neuroreport2001，12，： | 1 |
| 13 | Tissue specificity in rat peripheral nerve regeneration through combined skeletal muscle and vein conduit grafts 显示文摘 | TOS P BATTISTON B GEUNA S | 2000 | Microsurgery2000，20，2： | 1 |
| 14 | Nerve regeneration along bioengineered scaffolds显示文摘 | Geuna S Nicolino S Raimondo S | 2007 | Microsurgery2007，27，： | 1 |
| 15 | Lowpower laser biostimulation enhances nerve repair after end-to-side neurorrhaphy:a double blind randomized study in the rat median nerve model显示文摘 | Gigo-Benato D Geuna S de Castro Rodrigues A | 2004 | Lasers Med Sci2004，19，1： | 1 |
| 16 | Use of skeletal muscle tissue in peripheral nerve repair: review of the literature 显示文摘 | Meek MF Varejao AS Geuna S | 2004 | Tissue Eng2004，10，78： | 1 |
| 17 | Functional assessment of sciatic nerve recovery:biodegradable poly (DLLA-epsilon-CL) nerve guide filled with fresh skeletal muscle显示文摘 | Varejao AS Cabrita AM Geuna S | 2003 | Microsurgery2003，23，4： | 1 |
| 18 | Nerve repair by means of tubulization:literature review and personal clinical experience comparing biological and synthetic conduits for sensory nerve repair显示文摘 | Battiston B Geuna S Ferrere M ct al | 2005 | Microsurg2005，25，4： | 1 |
| 19 | Functional assessment of sciatic nerve recovery:biodegradable poly (DLLA-e-CL) nerve guide filled with fresh skeletal muscle显示文摘 | Varejao ASP Cabrita AM Geuna S | 2003 | Microsurgery2003，23，： | 1 |
| 20 | Appreciating the difference between design-based and model-based sampling strategies in quantitative morpbelogy of the nervous system显示文摘 | Geuna S | 2000 | J Comp Neurol2000，427，： | 1 |
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