|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Reversed-phase fused-core HPLC modeling of peptides显示文摘Different fused-core stationary phase chemistries(C18,Amide,Phenyl-hexyl and Peptide ES-C18) were used for the analysis of 21 structurally representative model peptides.In addition,the effects of the mobile phase composition(ACN or MeOH as organic modifier;formic acid or acetic acid,as acidifying component) on the column selectivity,peak shape and overall chromatographic performance were evaluated.The RP-amide column,combined with a formic acid-acetonitrile based gradient system,performed as best.A peptide reversed-phase retention model is proposed,consisting of 5 variables:log SumAA,log Sv,clog P,log nHDon and log nHAcc.Quantitative structure-retention relationship(QSRR) models were constructed for 16 different chromatographic systems.The accuracy of this peptide retention model was demonstrated by the comparison between predicted and experimentally obtained retention times,explaining on average 86% of the variability.Moreover,using an external set of 5 validation peptides,the predictive power of the model was also demonstrated.This peptide retention model includes the novel in-silico calculated amino acid descriptor,AA,which was calculated from log P,3D-MoRSE,RDF and WHIM descriptors. | Matthias D'Hondt Bert Gevaert Sofie Stalmans Sylvia Van Dorpe Evelien Wynendaele Kathelijne Peremans Christian Burvenich Bart De Spiegeleer | 2013 | Journal of Pharmaceutical Analysis2013，3，2： | 3 |
| 2 | The unsolved enigmas of leukemia inhibitory factor 显示文摘 | Metcalf D Bachert C Gevaert P | 2003 | Stem cells2003，21，： | 1 |
| 3 | Nasal IL-5levels determine the response to anti-IL-5treatment in patients with nasal polyps显示文摘 | Lang-Loidolt D Lackner A | 2006 | J Allergy Clin Immunol2006，118，5： | 1 |
| 4 | Tumour necrosis factor (TNF) gene polymorphism influences TNF-alpha production in lipopolysaecharide (LPS)-stimulated whole blood cell culture in healthy humans 显示文摘 | Louis E Franchimont D Prion A Gevaert Y Schaaf-Lafontaine N Roland S | 1998 | Clin Exp Immunol1998，113，3： | 1 |
| 5 | Global analysis of the mitochondrial Nproteome identifies a processing peptidase critical for protein stability显示文摘 | Voegtle FN Wortelkamp S Zahedi RP Becker D Leidhold C Gevaert K Kellermann J Voos W Sickmann A Pfanner N Meisinger C | | 0，，： | 1 |
| 6 | Gender,TIMI risk score and in-hospital mortality in STEMI patients undergoing primary PCI:results from the Belgian STEMI registry显示文摘 | Gevaert SA De Bacquer D Evrard P | 2014 | EuroIntervention2014，9，9： | 1 |
| 7 | Renal dysfunction in STEMI-patients undergoing primary angioplasty:higher prevalence but equal prognostic impact in female patients; an observational cohort study from the Belgian STEMI registry显示文摘 | Gevaert SA De Bacquer D Evrard P | | 0，，14： | 1 |
| 8 | Nasal IL-5 levels determine the response to anti-IL-5 treatment in patients with nasal polyps 显示文摘 | Gevaert P Lang-Loidolt D Lackner A | 2006 | J Allergy Clin Immunol2006，118，5： | 1 |
| 9 | Biometrics inLaminaria digitata: A useful tool to assess biomass,carbon and nitrogen contents 显示文摘 | Gevaert F Janquin M A Davoult D | 2008 | Journal of Sea Re-search2008，60，3： | 1 |
| 10 | Efficient solar cells based on an easily accessible diketopyn'olopyrrole polymer 显示文摘 | Bijleveld J C Gevaerts V S Di Nuzzo D | 2010 | Adv Mater2010，22，35： | 1 |
| 11 | The unsolved enigmas of leukemia inhibitory factor显示文摘 | Metcalf D Bachert C Gevaert P | 2003 | Stem Cells2003，21，： | 1 |
| 12 | Clinical evaluation of a robotic 6-degree of freedom treatment couch for frameless radiosurgery显示文摘 | Gevaert T Verellen D Engels B | 2012 | Int J Radiat Oncol Biol Phys2012，83，1： | 1 |
| 13 | Photo-inhibition and seasonal photosynthetic performance of the seaweed Laminaria saccharina during a simulated tidal cycle: chlorophyll fluorescence measurements and pigment analysis 显示文摘 | GEVAERT F CREACH A DAVOULT D | 2002 | Plant Cell Environ2002，25，： | 1 |
| 14 | Streptococcus bovis infections in pigeons 显示文摘 | DEVRIESE L A UYTYEBROEK E GEVAERT D | 1990 | Avian Pathol1990，19，3： | 1 |
| 15 | Predicting the Prognosis of Breast Cancer by Integrating Clinical and Mi- croarray Data with Bayesian Networks 显示文摘 | Gevaert O De Smet F Timmerman D | 2006 | Bioinformatics2006，22，14： | 1 |
| 16 | Derringer desirability and kinetic plot LC-column comparison approach for MS-compatible lipopeptide analysis显示文摘Lipopeptides are currently re-emerging as an interesting subgroup in the peptide research field,having historical applications as antibacterial and antifungal agents and new potential applications as antiviral, antitumor, immune-modulating and cell-penetrating compounds. However, due to their specific structure, chromatographic analysis often requires special buffer systems or the use of trifluoroacetic acid,limiting mass spectrometry detection. Therefore, we used a traditional aqueous/acetonitrile based gradient system, containing 0.1%(m/v) formic acid, to separate four pharmaceutically relevant lipopeptides(polymyxin B1, caspofungin, daptomycin and gramicidin A1), which were selected based upon hierarchical cluster analysis(HCA) and principal component analysis(PCA).In total, the performance of four different C18 columns, including one UPLC column, were evaluated using two parallel approaches. First, a Derringer desirability function was used, whereby six single and multiple chromatographic response values were rescaled into one overall D-value per column. Using this approach, the YMC Pack Pro C18 column was ranked as the best column for general MS-compatible lipopeptide separation. Secondly, the kinetic plot approach was used to compare the different columns at different flow rate ranges. As the optimal kinetic column performance is obtained at its maximal pressure,the length elongation factor λ(Pmax/Pexp) was used to transform the obtained experimental data(retention times and peak capacities) and construct kinetic performance limit(KPL) curves, allowing a direct visual and unbiased comparison of the selected columns, whereby the YMC Triart C18 UPLC and ACE C18columns performed as best. Finally, differences in column performance and the(dis)advantages of both approaches are discussed. | Matthias D’Hondt Frederick Verbeke Sofie Stalmans Bert Gevaert Evelien Wynendaele Bart De Spiegeleer | 2014 | Journal of Pharmaceutical Analysis2014，4，3： | 1 |
| 17 | Efficient solar cells based on an easily accessible diketopyrrolopyrrole poly- mer显示文摘 | Bijleveld B J Gevaert V S Nuzzo D D | 2010 | Adv Mater2010，22，： | 1 |
| 18 | Efficient solar cells based on an easily accessible diketopy- rrol opyrrole polymer显示文摘 | BIJLEVELD J C GEVAERTS V S Di Nuzzo D | 2010 | Advanced Material2010，22，35： | 1 |
| 19 | Efficient solar cells based on an easily accessible diketopyrrolopyrrole polymer显示文摘 | Bijleveld J C Gevaerts V S Di Nuzzo D | 2010 | Advanced Materials2010，22，35： | 1 |
| 20 | A novel receptor-mediated regulation mechanism of type Ⅰ inositol polyphosphate 5-phosphatase by calciunr/calmodulin-dependent protein kinase Ⅱ phosphorylation显示文摘 | Communi D Gevaert K Demol H | 2001 | J Biol Chem2001，276，38： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员