| 1 | Study and Application of Evaluation Method for Weather Impact of Traffic Accident on Zhejiang Freeway显示文摘Occurrence degree of the accident on Zhejiang freeway is graded. Evaluation indicator system of weather impact on freeway is established. We use principal component analysis to extract meteorological indicators,and use Logistic regression to establish evaluation model of meteorological indicator,thereby determining evaluation grade of traffic weather impact. Via application test,it is proved that the evaluation on traffic weather condition by the model corresponds with actual situation,which can provide certain decision-making basis for traffic department and the public. | Pan Yaying Zhu Zhanyun Shen Pingyue Luo Yuezhen Gu Tingting | 2014 | Meteorological and Environmental Research2014，5，11： | 0 |
| 3 | Engineering Photocleavable Protein-decorated Hydrogels to Regulate Cell Adhesion and Migration显示文摘Cell adhesion and migration play essential roles in tissue development and maintenance, and abnormal cell migration is involved in life-threatening diseases, including vascular disease, tumor formation, and metastasis. The advances in hydrogel-based 3D cell culture development facilitated the investigation of cell motility behavior, including cell-cell and cell-matrix adhesion and cell migration in a microenvironment more related to in vivo situations. Establishing advanced methods for these in vitro studies is thus necessary. Photo-sensitive proteins show advantages in remote and non-invasive regulation of hydrogels’ properties, and thus are of great potential in regulating 3D cultured cells’ behavior. In the presented study, we engineered photocleavable protein(PhoCl)-decorated hydrogels to regulate cell adhesion and migration of MDA-MB-231. The integrin-binding motif RGD was fused to the PhoCl and was decorated on the hydrogel. After being exposed to light at 405 nm, the PhoCl was cleaved and the RGD motif was released, resulting in detachment of the binding cells. The regulatory effect of the light illumination showed a time-dependent and cell density-dependent manner. Furthermore, the elimination of RGD by patterned light exposure completely suspended the cell migration to the corresponding region, suggesting a controllable regulation of the cell migration direction. | LI Tianyi HUANG Yaying LU Chenjing GU Liwen CAO Yi YIN Sheng | 2022 | Chemical Research in Chinese Universities2022，38，6： | 0 |
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