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您的检索式：作者名="GUIQING JI"
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| 1 | Impact of Asarum Essential Oils on Physiological and Biochemical Indicators of Two Agricultural Pests显示文摘[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall( chitin and phenoloxidase),the physiological and biochemical indices acting on the nervous system( Acetylcholinesterase),and the physiological and biochemical indices acting on the digestive system( protease,amylase and lipase) of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide. | Liu Yan Ji Lanzhu Wang Guiqing Wang Yuanxia Zhang Yue Yi Xuemei | 2014 | Plant Diseases and Pests2014，5，3： | 2 |
| 2 | Targeted editing of intronic-splicing silencer enhancement of SMN2 Exon 7 inclusion by CRISPR/Case 9显示文摘Spinal muscular atrophy(SMA)is an autosomal recessive hereditary neuromuscular disease.Exon 7 and 8 of survival of motor neuron 1(SMN1)gene or only exon 7 homology deletion leads to the failure to produce a full-length SMN gene.The copy number of SMN2 gene with high homology of SMN1 affects the degree of disease and was the target gene for targeting therapy,in which splicing silencer in intron 7 was the key to suppress the inclusion of exon 7.In this study,we projected to use CRISPR/Case 9 for the targeted editing of intronic-splicing silencer(ISS)sequence to promote the inclusion of SMN2 exon 7 and increase the production of SMN2 full-length(FL)gene expression.It happens that there was a protospacer adjacent motif(PAM)at one end of the ISS sequence according to the design of sgRNA.The recombinant vector of sgRNA HSMN2 CRISPR/Case 9 was constructed and transfected into HEK293 cells.Sequencing results showed that the ISS sequence could be edited accurately and targeting in the predicted direction,in which deleting small fragments,inserting small amounts and mutation.Quantitative analysis of RT-PCR products by restriction enzyme of DdeI digestion showed that the FL of SMN2 increased by 8%(P<0.05).In the primary cultured chondrocytes of SMA mice,in which sgRNA HSMN2 CRISPR/Case9 recombinant vector transfection could increase the SMN2 FL gene by 23%(P<0.05)and significantly improve SMN protein levels(P<0.05).CRISPR/Case 9 is an effective tool for gene editing and therapy of hereditary diseases,but it is rarely reported in the treatment of SMA diseases.This study shows that CRISPR/Case 9 was first used for the precision target of ISS sequence editing,which can effectively promote the production of SMN2 FL gene expressions,in which there was an important clinical reference value. | LIUCHENG WU YI WANG LILI DU GUIQING JI RUI ZHOU ZEYI ZHAO JUN CHEN SHUNXING ZHU | 2021 | BIOCELL2021，45，6： | 0 |
| 3 | Potential effects of elevated carbon dioxide on leaf-feeding forest insects显示文摘The elevated concentration of atmospheric CO_(2)may result in a decline of leaf nutritional quality(especially N)and an increase in some kinds of defensive secondary components(such as phenolics).The changes in the phytochemistry of trees,combined with the effect of elevated CO_(2)per se,have a potential negative influence on insect herbivores.Here,we review the effect of elevated CO_(2)on the performance of leaf-feeding forest insects at individual-level and commun-ity-level.The elevated CO_(2)per se have little influence on the metabolism of insects.Over half of the tree-insect experimental systems show that the performance of individual insect become poorer under high-CO_(2)grown trees;but the others show that the insects have just little or no response to the treatments.The direction and magnitude of the changes in the performance of insects could be mediated by various factors.The effects of treatment are strongly species-dependent.The mag-nitude of changes in the phytochemistry,the sensitivity and adaptive capacity of insects to the poorer leaf quality,the differences in plant growth conditions and experimental methods,and the mediated effects of other environmental factors(such as soil nutrient availability,light,temperature,O_(3))were all closely related to the final performance of insects.However,the larvae’s consumption usually increased under enriched CO_(2)treatment,which was widely thought to be a compens-atory response to poorer plant quality.The experiments on forest community-level found identically a reduction in herbivory,which was contrary to the results from small-scale experiments.The changes in insect popu-lation and the actual response of consumption by leaf-feeding forest insects under CO_(2)enrichment remain unclear,and more field-based experiments need to be conducted. | Xiaowei WANG Lanzhu JI Guiqing WANG Yan LIU | 2008 | Frontiers in Biology2008，3，1： | 0 |
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