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您的检索式：作者名="Gary WFelton"
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| 1 | 美洲棉铃虫幼虫腹侧可外翻腺分泌物对番茄抗虫反应的影响显示文摘为明确美洲棉铃虫Helicoverpa zea幼虫腹侧可外翻腺(ventral eversible gland,VEG)分泌物对植物抗虫反应的影响,在宾夕法尼亚州立大学帕克分校校园日光温室大棚内分别测定取食未损伤、机械损伤后涂抹磷酸缓冲盐溶液(phosphate buffer saline,PBS)和机械损伤后涂抹VEG分泌物3种处理的番茄叶片后美洲棉铃虫幼虫体重增加量,不同处理番茄叶片中防御关键酶多酚氧化酶(polyphenol oxidase,PPO)活性、胰蛋白酶抑制剂(trypsin proteinase inhibitors,TPI)抑制率以及防御相关的蛋白酶抑制剂Ⅱ(proteinase inhibitor Ⅱ,PIN2)基因相对表达量。结果显示,3种处理中,取食机械损伤后涂抹VEG分泌物的番茄叶片的美洲棉铃虫幼虫体重较处理前增加量最少,为4.27 mg,显著低于其它2种处理;机械损伤后涂抹VEG分泌物的番茄叶片中PPO活性和TPI抑制率最高,分别为109.16 U/mg和27.48%,分别显著高于其它2种处理;同时机械损伤后涂抹VEG分泌物的番茄叶片中PIN2基因相对表达量显著上调,分别是未损伤和机械损伤后涂抹PBS缓冲液处理的11.83倍和1.17倍。表明美洲棉铃虫幼虫VEG分泌物能够促使防御关键酶的大量积累,使得防御相关基因的相对表达量上调,显著诱导番茄的抗虫反应,阻止后续昆虫的取食。 | 潘秦剑 陈林 Michelle Peiffer Gary WFelton 刘同先 | 2019 | 植物保护学报2019，46，5： | 2 |
| 2 | Host permissiveness to baculovirus in flue nces time-dependent immune responses and fitness costs显示文摘Insects possess specific immune responses to protect themselves from different types of pathogens.Activation of immune cascades can inflict significant developmental costs on the surviving host.To characterize infection kinetics in a surviving host that experiences baculovirus inoculation,it is crucial to determine the timing of immune responses.Here,we investigated time-dependent immune responses and developmental costs elicited by inoculations from each of two wild-type baculoviruses,Autographa californica multiple nucleopolyhedrovirus(AcMNPV)and Helicoverpa zea single nucleopolyhedrovirus(HzSNPV),in their common host H.zea.As H.zea is a semi-permissive host of AcMNPV and fully permissive to HzSNPV,we hypothesized there are differential immune responses and fitness costs associated with resisting infection by each virus species.Newly molted 4th-instar larvae that were inoculated with a low dose(LD15)of either virus showed signify icantly higher hemolymph FAD-glucose dehydrogenase(GLD)activities compared to the corresponding control larvae.Hemolymph phenoloxidase(PO)activity,protein concentration and total hemocyte numbers were not increased,but instead were lower than in control larvae at some time points post-inoculation.Larvae that survived either virus inoculation exhibited reduced pupal weight;survivors inoculated with AcMNPV grew slower than the control larvae,while survivors of HzSNPV pupated earlier than control larvae.Our results highlight the complexity of immune responses and fitness costs associated with combating different baculoviruses. | Qinjian Pan Ikkei Shikano Gary WFelton Tong-Xian Liu Kelli Hoover | 2021 | Insect Science2021，28，1： | 1 |
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