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| 1 | Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens显示文摘Background: This study evaluated the effects of Eimeria spp. challenge and dietary supplementation with free methionine or methionine dipeptide on animal performance;expression of genes associated with the immune system, antioxidant system, and amino acid transport in the jejunum;and redox status of the jejunum of broiler chickens.Methods: A randomized, 2 × 3 factorial design was used, in which Eimeria spp. challenge was the first factor(Eimeria-challenged, EC, or unchallenged, UC, broilers) and methionine supplementation was the second factor(non-supplemented, NS;free DL-methionine, DL-Met;and methionine dipeptide, DL-methionyl-DL-methionine, DLMMet). At 14 days of age, chickens were inoculated orally with sporulated oocysts of Eimeria acervulina, Eimeria praecox, Eimeria maxima, and Eimeria mitis. Birds were killed by cervical dislocation 144 h post-inoculation(PI), and the jejunum was collected for biochemical and molecular analyses.Results: EC broilers had a 13% lower feed intake(FI), 37% lower body weight gain(BWG), and 39% higher feed conversion ratio(FCR) than UC broilers. Chickens fed the DL-Met diet had higher BWG(about 12% higher) and better FCR(about 12% lower) than chickens fed the NS diet. EC chickens had lower relative weight of the bursa of Fabricius(51.8%) and higher relative weights of the spleen and whole intestine(53.6% and 26.3%, respectively) than UC chickens. Eimeria spp. challenge led to an increase in the levels of oxidative substances, such as nitrite and thiobarbituric acid reactive substances(TBARS), in the jejunum of chickens 144 h PI. Among UC chickens, those fed the DL-Met diet had higher total antioxidant capacity(TAC) and lower catalase(CAT) and superoxide dismutase(SOD) activities. EC chickens that received the NS diet had higher carbonylated protein content(CP). This result was associated with their lower TAC and catalase activity. The lower TAC in EC chickens might have been due to reduced expression of catalase(CAT) and superoxide dismutase 1(SOD1) genes. Chickens fed the DL-Met and DLMMet diets had lower nitrite content. Eimeria spp. challenge suppressed neutral amino acid transporter 1(B0 AT1),peptide transporter 1(PEPT1), toll-like receptor 5(TLR5), interleukin 2(IL2), and occludin(OCLN) gene expression and enhanced cationic amino acid transporter 1(CAT-1) and interferon gamma(IFNG) gene expression. The highest PEPT1 expression level was observed in broilers fed the DL-MMet diet, and the lowest TLR5 expression level was found in broilers fed the NS diet.Conclusion: Our results show for the first time that supplementation with methionine as free amino acid or dipeptide helps protect the intestinal cells of broilers under Eimeria spp. challenge from the oxidative damage induced by free radicals, mainly through modulation of the antioxidant system. | Angélica de Souza Khatlab Ana Paula Del Vesco Adhemar Rodrigues de Oliveira Neto Roberta Pereira Miranda Fernandes Eliane Gasparino | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 6 |
| 2 | Age-related changes in mitochondrial UCP,ANT and COX HI gene expression in the breast muscleof quails ( Cotumix coturnix japonica) 显示文摘 | GASPARINO E DEL VESCO A P GUIMARAES SE | 2012 | GeneticsMolecular Research2012，11，3： | 1 |
| 3 | 查看详情显示文摘 | Erckmann V Gasparino U | | 0，，： | 1 |
| 4 | Dietary methionine effects on IGF-I and GHR m RNA expression in broilers显示文摘 | Del Vesco A P Gasparino E Oliveira Neto A R | 2013 | Genetics and Molecular Research2013，12，4： | 1 |
| 5 | Effect of methionine supplementation on mitochondrial genes expression in the breast muscle and liver of broilers显示文摘 | Del V A P Gasparino E Oliveira N A R | 2013 | Livestock Science2013，151，23： | 1 |
| 6 | Ghosts of e-mails continue to haunt Wall Street-In Grubman inquii'y, preschool is pressed on twins' admission 显示文摘 | Gasparino Charles | 2002 | Wall Street Journal2002，11，： | 1 |
| 7 | Expression of calpastatin and myostatin genes associated with lamb meat quality显示文摘 | BAGATOLI A GASPARINO E SOARES M | 2013 | Genetics and Molecular Research2013，12，4： | 1 |
| 8 | Dietary methionine effects on IGF-I and GHR mRNA expression in broilers显示文摘 | Del Vesco A P Gasparino E Oliveira Neto A R | 2013 | Genetics and Molec ular Research2013，12，4： | 1 |
| 9 | Effect of methionine supplementation on mitoehondrial genes expression in the breast muscle and liver of broilers显示文摘 | VESCO A P D GASPARINO E NETO A R O | 2013 | Livestock Science2013，，151： | 1 |
| 10 | Effect of methionine supplementation on mitochondrial genes expression in the breast muscle and liver of broilers显示文摘 | Del Vesco A P Gasparino E Oliveira N A R | 2013 | Livestock Science2013，151，23： | 1 |
| 11 | Production of reactive oxygen species, gene expression, and enzymatic activity in quail subjected to acute heat stress 显示文摘 | DEL VESCO A P GASPARINO E | 2013 | J Anita Sci2013，91，2： | 1 |
| 12 | Polymorphism in the GH1-PstI gene associated to corporal characteristics in nile tilapia strains 显示文摘 | Blanck D V Gasparino E R Ricardo P | 2009 | Pesquisa Agropecufria Brasileira2009，44，6： | 1 |
| 13 | Effect of methionine supplementationon mitochondrial genes expression in the breast muscleand liver of broilers显示文摘 | DEL-VESCO A P GASPARINO E OLIVEIRA-NE-TO A R | 2013 | Livestock Science2013，151，23： | 1 |
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