维普中文期刊产品整合服务
13篇 您的检索式:作者名="Gehr M E"
    题名 作者 年代 出处 被引量
1Numerical Analysis of Coaxial Double Gate Schottky Barrier Carbon Nanotube Field Effect Transistors显示文摘Pourfath M Ungersboeck E Gehring A 2005Journal of Computational Electronics2005,,4:1
2Numerical simulations and experimental characterization of heat transfer from a periodic impingement of droplets显示文摘Trnjillo M F Alvarado J Gehring E 2011Journal of Heat Transfer2011,133,12:1
3Numerical simulations and experimental characterization of heat transfer from a periodic impingement of droplets显示文摘Trujillo M F Alvarado J Gehring E 2011Journal of Heat Transfer2011,133,12:1
4Decomposing ERP time-frequency energy using PCA显示文摘BERNAT E M WILLIAMS W J GEHRING W J 2005Clinical Neurophysiology2005,116,:1
5Cell number and cell characteristics of the normal human lung 显示文摘Crap J D Barry B E Gehr M 1982Am Rev Respir Dis1982,126,:1
6Childhood cat allergen exposure in three European countries:The AIRALLERG study显示文摘Giovannangelo M Gehring U Nordling E 2006Sci Total Environ2006,369,:1
7Effect of nitrogen content on the degradation mechanisms of thin Ta-Si-N diffusion barriers for Cu metallization显示文摘HUBNER R HECKER M MATTERN N HOFFMANN V WETZIG K HEUER H WENZEL C ENGELMANN H J GEHRE D ZSCHECH E 2006Thin Solid Films2006,500,12:1
8Pharmacokinetics and bioavailability of sulfadiazine and trimethoprim following intravenous, intramuscular and oral administration in ostriches ( Struthio camelus ) 显示文摘ABU-BASHA E A GEHRING R HANTASH T M et at 2009J Vet Pharmacol Ther2009,32,3:1
9Phar- macokinetics and bioavailability of sulfadiazine and trime- thoprim following intravenous, intramuscular and oral ad- ministration in ostriches ( Struthiocamelus ) 显示文摘Abu -basha E A Gehring R Hantash T M 2009J Vet Pharmacol Ther2009,32,3:1
10Iron acquisition in plague : modular logic in enzymatic biogenesis of yersiniabactinby Yersinia pestis显示文摘Gehring A M DeMoll E Fetherston J D 1998Chem Biol1998,5,10:1
11A neural system for error detection and compensation 显示文摘Gehring W Goss B Coles M Meyer D Donchin E 1993Psychological science1993,4,6:1
12The dimensions of the human dentogingival junction 显示文摘Vacek J S Gehr M E Asad DA 1994International Journal of Periodontics and Restorative Dentistry1994,14,:1
13Laser capture microdissection and genetic analysis of carbon-labeled Kupffer cells显示文摘AIM: To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection (LCM). METHODS: Tissues are complex structures comprised of a heterogeneous population ofinterconnected cells. LCM offers a method ofisolating a single cell type from specific regions of a tissue section. LCM is an essential approach used in conjunction with molecular analysis to study the functional interaction of cells in their native tissue environment. The process of labeling and acquiring cells by LCM prior to mRNA isolation can be elaborate, thereby subjecting the RNA to considerable degradation. Kupffer cell labeling is achieved byinjecting India ink intravenously, thus circumventing the need for in vitro staining. The significance of this novel approach was validated using a cholestatic liver injury model. RESULTS: mRNA extracted from the microdissected cell population displayed marked increases in colonystimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer cells derived from bile-duct-ligated, versus sham-operated, mice was compared. Microarray analysis revealed a significant (2.5-fold, q value < 10) change in 493 genes. Based on this fold-change and a standardized PubMed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury. CONCLUSION: The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.Stephan Gehring Edmond Sabo Maryann E San Martin Elizabeth M Dickson Chao-Wen Cheng Stephen H Gregory 2009World Journal of Gastroenterology2009,15,14:0
返回顶部 每页显示:
共1页 首页 上一页 第1页 下一页 末页 /1 跳转

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1 渝公网安备 50019002500403号 违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费