|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Enhancement of LTP in aged rats is dependent on endogenous BDNF显示文摘 | Diógenes M J Costenla A R Lopes L V | 2011 | Neuropsychopharmacology2011，36，9： | 1 |
| 3 | Meta-analysis of Research on Class Size and Its Relationship to Attitudes and Instruction 显示文摘 | Mary Lee Smith & Gene V Glass | 1980 | American Educational Research Journal1980，17，4： | 1 |
| 4 | Meta-analysis of Research on Class Size and Its Relationship to Attitudes and Instruction显示文摘 | Mary Lee Smith & Gene V Glass | 1980 | American Educational Research Journal1980，17，4： | 1 |
| 5 | Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats显示文摘 | Melissa V Ingrid B Genes | 2000 | Am J Physiol Gastrointest Liver Physiol2000，279，5： | 1 |
| 6 | Flooding for reliable multicast in multi-hop Ad hoe networks显示文摘 | Katia O Kumar V Gene T | 2001 | Wireless Networks2001，7，6： | 1 |
| 7 | Primary, Secondary and Meta-analysis of Research 显示文摘 | Gene V Glass | 1976 | Educational Researcher1976，，5： | 1 |
| 8 | Decision tree-basedpreventive and corrective control applications fordynamic security enhancement in power systems显示文摘 | Gene I Diao R Vittal V | 2010 | IEEE Trans on Power Systems2010，25，3： | 1 |
| 9 | What necessitates theconversion to open cholecystectomy ? A retrospective analysis of5 164 consecutive laparoscopic operations 显示文摘 | Gene V Sulaimanov M Cipe G | 2011 | Clinics2011，66，： | 1 |
| 10 | Nef in cholesterol synthesis and upvirus type 1-infected T cells显示文摘 | VAN'T WOUT A B SWAIN J induces multiple genes involved take in human immunodeficiency V SCHINDLER M | 2005 | J Virol2005，79，10： | 1 |
| 11 | Retrospective review of total neoadjuvant therapy显示文摘BACKGROUND Neoadjuvant chemoradiotherapy(nCRT)followed by resection and postoperative multi-agent chemotherapy(maChT)is the standard of care for locally advanced rectal cancer.Using this approach,maChT administration can be delayed for several months,leading to concern for distant metastases.To counteract this,a novel treatment approach known as total neoadjuvant therapy(TNT)has gained popularity,in which patients receive both maChT and nCRT prior to resection.We utilized the National Cancer Database to examine temporal trends in TNT usage,and any potential effect on survival.AIM To study the temporal trends in the usage of TNT and evaluate its efficacy compared to neoadjuvant chemoradiation.METHODS We queried the National Cancer Database for patients with locally advanced rectal cancer,Stage II-III,from 2004-2015 treated with nCRT or TNT.TNT was defined as maChT initiated≥90 d prior to nCRT initiation.Overall survival was calculated from the date of diagnosis to the date of last contact or death using Kaplan-Meier curves to present the cumulative probability of survival,with logrank statistics to assess significance.Multivariable cox regression was used to identify predictors of survival and propensity score analysis accounted for bias.RESULTS We identified 9066 eligible patients,with 8812 and 254 patients receiving neoadjuvant chemoradiation followed by maChT and TNT,respectively.Nodal involvement,stage III disease,and treatment in recent years were predictive of TNT use.There was greater use of TNT with more advanced stage,specifically>1 node involved(odds ratio[OR]=2.88,95%confidence interval[CI]:2.11-3.93,P<0.01)and stage III disease(OR=2.88,95%CI:2.11-3.93,P<0.01).From 2010 to 2012 the use of TNT increased(OR=2.41,95%CI:1.27-4.56,P<0.01)with a greater increase from 2013 to 2015(OR=6.62,95%CI:3.57-12.25,P<0.01).Both the TNT and neoadjuvant chemoradiation arms had a similar 5-year survival at 76%and 78%respectively.Multivariable analysis with propensity score demonstrated that increased age,high comorbidity score,higher grade,African American race,and female gender had worse overall survival.CONCLUSION Our data demonstrates a rising trend in TNT use,particularly in patients with worse disease.Patients treated with TNT and nCRT had similar survival.Randomized trials evaluating TNT are underway. | Laila Babar Veli Bakalov Stephen Abel Obaid Ashraf Gene Grant Finley Moses S Raj Kristina Lundeen Dulabh K Monga Alexander V Kirichenko Rodney E Wegner | 2019 | World Journal of Gastrointestinal Oncology2019，11，10： | 1 |
| 12 | Real-world cure rates for hepatitis C virus treatments that include simeprevir and/or sofosbuvir are comparable to clinical trial results显示文摘AIM To assess the real-world effectiveness and cost of simeprevir(SMV), and/or sofosbuvir(SOF)-based therapy for chronic hepatitis C virus(HCV) infection.METHODS The real-world performance of patients treated with SMV/SOF ± ribavirin(RBV), SOF/RBV, and SOF/RBV with pegylated-interferon(PEG) were analyzed in a consecutive series of 508 patients with chronic HCV infection treated at a single academic medical center. Patients with genotypes 1 through 4 were included. Rates of sustained virological response-the absence of a detectable serum HCV RNA 12 wk after the end of treatment [sustained virological response(SVR) 12]-were calculated on an intention-to-treat basis. Costs were calculated from the payer's perspective using Medicare/Medicaid fees and Redbook Wholesale Acquisition Costs. Patient-related factors associated with SVR12 were identified using multivariable logistic regression.RESULTS SVR 12 rates were as follows: 86%(95%CI: 80%-91%)among 178 patients on SMV/SOF ± RBV; 62%(95%CI: 55%-68%) among 234 patients on SOF/RBV; and 78%(95%CI: 68%-86%) among 96 patients on SOF/PEG/RBV. Mean costs-per-SVR 12 were $174442(standard deviation: ± $18588) for SMV/SOF ± RBV; $223003(± $77946) for SOF/RBV; and $126496(± $31052) for SOF/PEG/RBV. Among patients on SMV/SOF ± RBV, SVR12 was less likely in patients previously treated with a protease inhibitor [odds ratio(OR): 0.20, 95%CI: 0.06-0.56]. Higher bilirubin(OR: 0.47, 95%CI: 0.30-0.69) reduced the likelihood of SVR12 among patients on SOF/RBV, while FIB-4 score ≥ 3.25 reduced the likelihood of SVR 12(OR: 0.18, 95%CI: 0.05-0.59) among those on SOF/PEG/RBV. CONCLUSION SVR 12 rates for SMV and/or SOF-based regimens in a diverse real-world population are comparable to those in clinical trials. Treatment failure accounts for 27% of costs. | Kian Bichoupan Neeta Tandon James F Crismale Joshua Hartman David Del Bello Neal Patel Sweta Chekuri Alyson Harty Michel Ng Keith M Sigel Meena B Bansal Priya Grewal Charissa Y Chang Jennifer Leong Gene Y Im Lawrence U Liu Joseph A Odin Nancy Bach Scott L Friedman Thomas D Schiano Ponni V Perumalswami Douglas T Dieterich Andrea D Branch | 2017 | World Journal of Virology2017，6，4： | 1 |
| 13 | Modeling a lethal prostate cancer va- riant with small-cell carcinoma features 显示文摘 | gene Tzelepi V Zhang J Lu JF | 2012 | Clin Cancer Res2012，18，3： | 1 |
| 14 | Cytokines profile and metabolic activity of neutrophils of peripheral blood when progressing neoplasma显示文摘 | Abakumova T V Antoneeva I I Gening T P | 2014 | Patol Fiziol Eksp Ter2014，20，4： | 1 |
| 15 | Association between high activity of DNA polymerase iota and the development of human uveal melanoma显示文摘 | Gening L V Grishina E E Petrochenkov A N | 2006 | Genetika2006，42，1： | 1 |
| 16 | Quantitative imaging biomarkers: A review of statistical methods for technical performance assessment显示文摘 | Raunig David L McShane Lisa M Pennello Gene Gatsonis Constantine Carson Paul L Voyvodic James T Wahl Richard L Kurland Brenda F Schwarz Adam J G?nen Mithat Zahlmann Gudrun Kondratovich Marina V O’Donnell Kevin Petrick Nicholas Cole Patricia | 2015 | Statistical Methods in Medical Research2015，，： | 1 |
| 17 | Analysis of islet regenerating(reg)gene polymorphisms in fibrocalculous pancreatic diabetes显示文摘 | A gene Hawrami K Mohan V Bone A | 1997 | Pancreas1997，14，2： | 1 |
| 18 | Synthesis and characterization of new strontium 4-carboxyphenylphosphonates 显示文摘 | Zima V Svoboda J genes L | 2007 | Journal of Solid State Chemistry2007，1180，3： | 1 |
| 19 | Teachers' Evaluation 显示文摘 | Gene V Glass | 2004 | Policy Brief:ERIC Document Reproduction Service2004，，： | 1 |
| 20 | PCC 6803显示文摘 | NEFEDOVA L N FANTIN Y S ZINCHENKO V V TheprqA and mvrA genes encoding drug efflux proteins controlresistance to methyl viologen in the cyanobacterium Synechocystissp | 2003 | Russ J Genet2003，39，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员