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| 1 | Angiogenesis in rabbit hepatic tumor after transcatheter arterial embolization显示文摘AIM: To investigate the effect of transcatheter arterial embolization (TAE) on angiogenesis of hepatic tumor.METHODS: Twenty New Zealand White rabbits were randomly divided into two groups of 10 each and VX2 carcinoma was implanted in the left medial lobes of the livers. Fourteen days later, a silicon catheter was inserted into the left hepatic artery of rabbit with VX2 hepatic tumor and infusion was performed via the hepatic artery using Lipiodol (the TAE group) or saline (the control group). Rabbits were sacrificed 7 d after treatment and tumor tissues were excised. Expression of vascular endothelial growth factor (VEGF) protein and microvessel density (MVD) of tumors were examined using immunohistochemistry. The staining intensity of VEGF was evaluated with a computer-assisted image-analyzer. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the VEGF mRNA expression of tumors.RESULTS: MVD was higher in the TAE group compared with the control group (28.6±10.6 vs 16.3±6.9, P<0.01).Expression of VEGF protein was enhanced after TAE. The staining intensity of VEGF in the TAE group was 0.162±0.018,significantly higher than in the control group (0.142±0.01,P<0.01). At mRNA level, VEGF165 mRNA was significantly higher in the TAE group compared with the control group (2.58±0.42 vsl.99±0.21, P<0.001). MVD was well correlated to VEGF expression in both the TAE group (r=0.69, P<0.05) and the control group (r=-0.72, P<0.05).CONCLUSION: TAE promotes the development of neovascularization of residual tumors through up-regulation of VEGF expression, possibly due to hypoxic insult. | Xiao-FengLiao Ji-LinYi Xing-RuiLi WeiDeng Zhi-FangYang GengTian | 2004 | World Journal of Gastroenterology2004，10，13： | 11 |
| 2 | Antitumor immunopreventive effect in mice induced by DNA vaccine encoding a fusion protein of α-fetoprotein and CTLA4显示文摘AIM:To develop a tumor DNA vaccine encoding a fusion protein of murine AFP and CTLA4,and to study its ability to induce specific CTL response and its protective effect against AFP-producing tumor.METHODS:Murine α-fetoprotein (mAFP) gene was cloned from total RNA of Hepal-6 cells by RT-PCR.A DNA vaccine was constructed by fusion murine α-fetoprotein gene and extramembrane domain of murine CTLA4 gene.The DNA vaccine was identified by restriction enzyme analysis,sequencing and expression.EL-4 (mAFP) was developed by stable transfection of EL-4 cells with pmAFP.The frequency of cells producing IFN-γ in splenocytes harvested from the immunized mice was measured by ELISPOT.Mice immunized with DNA vaccine were inoculated with EL-4(mAFP) cells in back to observe the protective effect of immunization on tumor. On the other hand,blood samples were collected from the immunized mice to check the functions of liver and kidney.RESULTS:1.8kb mAFP cDNA was cloned from total RNA of Hepal-6 cells by RT-PCR.The DNA vaccine encoding a fusion protein of mAFP-CTLA4 was constructed and confirmed by restriction enzyme analysis,sequencing and expression.The expression of mAFP mRNA in EL-4 (mAFP) was confirmed by RT-PCR.The ELISPOT results showedthat the number of IFN-γ-producing cells in pmAFP-CTLA4 group was significantly higher than that in pmAFP,pcDNA3.1 and PBS group.The tumor volume in pmAFP-CTLA4 group was significantly smaller than that in pmAFP,pcDNA3.1 and PBS group, respectively. The hepatic and kidney functions in each group were not altered.CONCLUSION: AFP-CTLA4 DNA vaccine can stimulate potent specific CTL responses and has distinctive antitumor effect on AFP-producing tumor.The vaccine has no impact on the function of mouse liver and kidney. | GengTian Ji-LinYi PingXiong | 2004 | World Journal of Gastroenterology2004，10，2： | 5 |
| 3 | Project Systems Engineering for Developing Space Robots显示文摘Space robots possess unique distinguishing features unlike general robots on earth, due to the particular environments in space. The developing of various practical space robots promoting the improvement of space science and technology is a complex man-machine-environment engineering problem. This paper analyses from the systems engineering viewpoint the space robot system in the scope of the architecture of robotics discipline, space environment characteristics, man-machine-environment system of space robots, the general methodology of project systems engineering and the process of space robot systems engineering. | Li Gengtian, Li ZhibinBeijing Institute of Control Engineering, P.O.Box 2729, Beijing 100080, ChinaLü YingxiangBeijing Institute of Electromechanic System Engineering.P.O.Box 142-206, Beijing 100854, China | 1991 | Journal of Systems Engineering and Electronics1991，2，1： | 0 |
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