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| 1 | Stool-based DNA testing,a new noninvasive method for colorectal cancer screening,the first report from Iran显示文摘AIM:To detect tumor-associated DNA changes in stool samples among Iranian patients with colorectal cancer(CRC)compared to healthy individuals using BAT-26,p16 hypermethylation and long DNA markers.METHODS:Stool DNA was isolated from 45 subjects including 25 CRC patients and 20 healthy individuals using a new,fast and easy extraction method.Long DNA associated with tumor was detected using polymerase chain reaction method.Microsatellite studies were performed utilizing denaturating polyacrylamide gel to determine the instability of BAT-26.Methylation status of p16 promoter was analyzed using methylation-specific PCR(MSP).RESULTS:The results showed a significant difference in existence of long DNA(16 in patients vs 1 in controls,P < 0.001)and p16(5 in patients vs none in controls,P = 0.043)in the stool samples of two groups.Long DNA was detected in 64% of CRC patients;whereas just one of the healthy individuals was positive for Long DNA.p16 methylation was found in 20% of patients and in none of healthy individuals.Instability of BAT-26 was not detected in any of stool samples.CONCLUSION:We could detect colorectal cancer related genetic alterations by analyzing stool DNA witha sensitivity of 64% and 20% and a specificity of 95% and 100% for Long DNA and p16 respectively.A non-invasive molecular stool-based DNA testing can provide a screening strategy in high-risk individuals.However,additional testing on more samples is necessary from Iranian subjects to determine the exact specificity and sensitivity of these markers. | Mohammad Reza Abbaszadegan Alireza Tavasoli Arash Velayati Hamid Reza Sima Hassan Vosooghinia Mehdi Farzadnia Hamid Asadzedeh Mehran Gholamin Ezzat Dadkhah Azadeh Aarabi | 2007 | World Journal of Gastroenterology2007，13，10： | 14 |
| 2 | Overexpression and interactions of interleukin-10, transforming growth fac- tor beta, and vascular endothelial growth factor in esopha- geal squamous cell carcinoma显示文摘 | Gholamin M Moaven O Memar B | 2009 | World J Surg2009，33，7： | 1 |
| 3 | Effects of polyeth- ylene glycol and NaC1 stress on two cultivars of wheat ( Triticum durum) at germination and early seeding stages 显示文摘 | GHOLAMIN R KHAYATNEZHAD M | 2010 | Am-Eur J Agric & Environ Sci2010，9，1： | 1 |
| 4 | Medical versus medical and surgical treatment for brucella endoeardi'ds 显示文摘 | Keshtkar-Jahromi M Razavi SM Gholamin S | 2012 | AnnThoracSurg2012，94，6： | 1 |
| 5 | Medical versus medical and surgical treatment for brucella endocarditis显示文摘 | Keshtkar-Jahromi M Razavi SM Gholamin S | 2012 | Ann Thorac Surg2012，94，6： | 1 |
| 6 | Induction of cytotoxic T lymphocytes primed with tumor RNA-loaded dendritic cells in esophageal squamous cell carcinoma:preliminary step for DC vaccine design显示文摘 | Gholamin M Moaven O Farshchian M | | 0，，： | 1 |
| 7 | Overexpression and interactions of interleukin-10,Transforming growth factorβ,and vascular endothelial growth factor in esophageal squamous cell carcinoma显示文摘 | Gholamin M Moaven O Memar B | 2009 | World J Surg2009，33，7： | 1 |
| 8 | Medical versus medical and surgical treatment for brucella endocarditis 显示文摘 | Keshtkar-Jahromi M Razavi SM Gholamin S | 2012 | Ann Thorac Surg2012，94，6： | 1 |
| 9 | Cancer-testis gene expression profiling in esophageal squamous cell carcinoma: identification of specific tumor marker and potential targets for immunotherapy 显示文摘 | Forghanifard MM Gholamin M Farshchian M | 2011 | Cancer Biol Ther2011，12，3： | 1 |
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