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| 1 | HuR mediated post-transcriptional regulation as a new potential adjuvant therapeutic target in chemotherapy for pancreatic cancer显示文摘AIM: To investigate the expression of Hu R in pancreatic ductal adenocarcinoma(PDA) and to assess the effects of Hu R silencing on the expression of cyclooxygenase-2(COX-2) and heme oxygenase-1(HO-1) and the in vitro response to gemcitabine(GEM) treatment in pancreatic cell lines.METHODS: We compared the expression of Hu R,COX-2,and HO-1 in PDA and normal pancreatic tissueusing quantitative reverse transcription polymerase chain reaction(q RT-PCR) and western blot. In addition,the Hu R,COX-2 and HO-1 were analyzed in four types of cancer cell lines(Mia Paca2,Su.86.86,Capan-1,and Capan-2) with and without GEM treatment. Immunocytofluorescence analysis was used to investigate Hu R localization in cells. Cell viability and response to GEM after Hu R silencing were determined with the 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test and the crystal violet clonogenic assay,respectively. To measure apoptosis,activation of caspases 3/7 was evaluated using immunofluorescence. RESULTS: In PDA tissue obtained from patients not treated with GEM,Hu R m RNA expression was 3.2 times lower(P < 0.05) and COX-2 and HO-1 m RNAexpression was 2.3-fold and 7.2-fold higher(P < 0.05),respectively,than normal pancreatic tissue(from organ donor). q RT-PCR analysis showed that Hu R,COX-2,and HO-1 m RNA were overexpressed in all cancer cell lines treated with the half maximal inhibitory concentration(IC50) dose of GEM compared with control cells(P < 0.05). Western blot analysis revealed that COX-2 and HO-1 levels were significantly decreased in cancer cells after Hu R silencing. Furthermore,HuR silencing in creased the response to GEM treatmentand decreased cell viability by 11.6%-53.7% compared to control cell lines. Caspases 3 and 7 were activated after Hu R silencing and GEM treatment in all pancreatic cancer cell lines. In comparison,treatment with GEM alone did not activate caspases 3 and 7 in the same cell lines. CONCLUSION: Hu R mediated post-transcriptional upregulation of COX-2 and HO-1 expression after GEM treatment in pancreatic cancer cells. Hu R silencing significantly increased the effectiveness of GEM treatment in vitro. | Aldona Jakstaite Aurelija Maziukiene Giedre Silkuniene Kristina Kmieliute Antanas Gulbinas Zilvinas Dambrauskas | 2015 | World Journal of Gastroenterology2015，21，46： | 4 |
| 2 | Ultra-broadband all-OPCPA petawatt facility fully based on LBO显示文摘A petawatt facility fully based on noncollinear optical parametric chirped pulse amplification(NOPCPA)technology,Vulcan OPPEL(Vulcan OPCPA PEtawatt Laser),is presented.This system will be coupled with the existing hybridCPA/OPCPA VULCAN laser system(500 J,500 fs beamline;250 J,ns regime beamline)based on Nd:glass amplification.Its pulse duration(20 times shorter)combined with the system design will allow the auxiliary beamline and its secondary sources to be used as probe beams for longer pulses and their interactions with targets.The newly designed system will be mainly dedicated to electron beam generation,but could also be used to perform a variety of particle acceleration and optical radiation detection experimental campaigns.In this communication,we present the entire beamline design discussing the technology choices and the design supported by extensive simulations for each system section.Finally,we present experimental results and details of our commissioned NOPCPA picosecond front end,delivering 1.5 mJ,~180 nm(1/e^(2))of bandwidth compressed to sub-15 fs. | Mario Galletti Pedro Oliveira Marco Galimberti Munadi Ahmad Giedre Archipovaite Nicola Booth Emerald Dilworth Andy Frackiewicz Trevor Winstone Ian Musgrave Cristina Hernandez-Gomez | 2020 | High Power Laser Science and Engineering2020，8，4： | 2 |
| 3 | Effect of short-wavelength light on lettuce growth and nutritional quality显示文摘 | Akvile U Paulo P Giedre S | 2007 | Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agrlculture2007，26，1： | 1 |
| 4 | Congestive hepatopathy and hypoxic hepatitis in heart failure: A cardiologist’s point of view显示文摘 | Ausra Kavoliuniene Audrone Vaitiekiene Giedre Cesnaite | 2013 | International Journal of Cardiology2013，，3： | 1 |
| 5 | Feasibility of CHP-plants with thermal stores in the German spot market显示文摘 | Giedr? Streckien? Vytautas Martinaitis Anders N. Andersen Jonas Katz | 2009 | Applied Energy2009，，11： | 1 |
| 6 | The effect of red and blue light component on the growth and development of frigo strawberries显示文摘 | GIEDRE SAMUOLIENE AUSRA BRAZAITYTE AKVILE URBONAVIVIUTE | 2010 | Zemdirbyste Agricuhure2010，97，2： | 1 |
| 7 | Significance of blood serum catalase activity and malondialdehyde level for survival prognosis of ovarian cancer patients显示文摘 | JANINA DIDIAPETRIENE JAROSLAV BUBLEVIC GIEDRE SMAILYTI | 2014 | Medicina2014，60，4： | 1 |
| 8 | Risk factors for clinical anastomotic leak age following the resectionof sigrnoid and rectal cancer显示文摘 | Giedre Rudinskaite Algimantas Tamelis Zilvinas Saladzinskas | 2005 | Medicina( Kaunas )2005，41，9： | 1 |
| 9 | Capillary electrophoretic determination of sulfite using the zone-passing technique of in-capillary derivatization显示文摘 | Giedre Jankovskiene Zydrunas Daunoravicius Audrius Padarauskas | 2001 | Journal of Chromatography A2001，，： | 1 |
| 10 | Small molecule activators of SIRT1 replicate signaling pathways triggered by calorie restriction in vivo 显示文摘 | Jesse J Smith Renee Deehan Kenney David J Gagne Brian P Frushour William Ladd Heidi L Galonek Kristine Israelian Jeffrey Song Giedre Razvadauskaite Amy V Lynch David P Carney Robin J Johnson Siva Lavu Andre Iffland Peter J Elliott Philip D Lambert Keith O Elliston Michael R Jirousek Jill C Milne and Olivier Boss | 2009 | BMC Systems Biology2009，284，27： | 1 |
| 11 | LED illumination affects bioactive compounds in romaine baby leaf lettuce显示文摘 | Giedr? Samuolien? Au?ra Brazaityt? Ramūnas Sirtautas Akvil? Vir?il? Jurga Sakalauskait? Sandra Sakalauskien? Pavelas Duchovskis | 2013 | J Sci Food Agric2013，，13： | 1 |
| 12 | LED lighting and seasonality effects antioxidant properties of baby leaf lettuce显示文摘 | Giedr? Samuolien? Ramūnas Sirtautas Au?ra Brazaityt? Pavelas Duchovskis | 2012 | Food Chemistry2012，，3： | 1 |
| 13 | Interaction of lead (Ⅱ) with β-cyclodextrin in alkaline solutions 显示文摘 | Eugenijus N Giedre G Rimantas V | 2002 | Carbohydrate Research2002，337，： | 1 |
| 14 | Interaction of β-cyclodextrin with cadmium ( Ⅱ ) ions 显示文摘 | Eugenijus N Giedre G Rimantas V | 2003 | International Journal of Biological Macromolecules2003，33，： | 1 |
| 15 | Millets across Eurasia: chronology and context of early records of the genera Panicum and Setaria from archaeological sites in the Old World显示文摘 | Harriet V. Hunt Marc Vander Linden Xinyi Liu Giedre Motuzaite-Matuzeviciute Sue Colledge Martin K. Jones | 2008 | Vegetation History and Archaeobotany2008，，1： | 1 |
| 16 | Quality of life of Lithuanian women with early stage breast cancer显示文摘 | Giedre Bulotiene Jonas Veseliunas Valerij Ostapenko | 2007 | BMC Public Health2007，26，7： | 1 |
| 17 | Matrix solid - phasedispersion extraction of carbadox and olaquindox in feed followed by hydro- philic interaction ultra -high -pressure liquid chromatographic analysis显示文摘 | Giedre Kesirmaite Evaldas Naujalis Audrius Pada- rauskas | 2008 | Journal of Chromatog- raphy A2008，1209，1： | 1 |
| 18 | Risk factors for clinical anastomotic leakage following the resection of sigmnid and rectal cancer显示文摘 | Giedre Rudinskaite Algimantas Tamelis Zilvinas Saladzinskas | 2005 | Medicina (Kaunas)2005，41，9： | 1 |
| 19 | The effeet of red and blue light com- ponent on the growth and developmen! of frigo strawberries 显示文摘 | Giedre SAMUOLIENE Ausra BRAZAITYTE Akvile UR- BONAVIVIUTE | 2010 | Zemdi rbyste-Agriculture2010，7，2： | 1 |
| 20 | Riak factors for clinical anastomotic leakage following the resection of sigmoid and tectal cancer显示文摘 | Giedre Rudinskaite Algimantas Tamelis Zilvinas Saladzinskaa | 2005 | Medicina (Kaunas)2005，41，9： | 1 |
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